We narrowed to 525 results for: MEA

1. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

   Type

   Blog Post

   ...Furthermore, CRISPR/Cas9 editing is often bi-allelic, meaning that even if one allele is accurately edited, the...

2. CRISPR 101: Homology Directed Repair

   Type

   Blog Post

   ...which exclusively yields non-crossover events. This means all newly synthesized sequences are retained by ...

3. An Integrin Antibody Toolkit from IPI

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   Blog Post

   ...With the right research reagents, researchers can measure this conformational change to investigate the role...

4. Multiple Plasmids at a Low Price: Kits from Addgene

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   Blog Post

   ...sites used in these vectors are of low abundance (meaning they are unlikely to be found in your gene of interest...

5. Site Directed Mutagenesis by PCR

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   Blog Post

   ...resistance marker from the parental plasmid provides a mean for selecting for transformants which have taken...

6. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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   Blog Post

   ... the percentage of cells with activated HIV-1, measured via HIV-1 promoter-driven GFP expression. In cell...

7. Gamma-Retroviral Vector Guide

   Type

   Guide

   ...only infect mitotically active cell types. This means that lentiviruses can infect a greater variety of...

8. Is this the right place for me? 8 tactics for choosing a lab

   Type

   Blog Post

   ...failure to acknowledge good work, allocation of meaningless tasks, removal of responsibility, repeated reminders...

9. Viral Vectors 101: Optogenetic Tools

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   Blog Post

   ...activity or gene expression. Experimental output is measured and quantified. Created with BioRender.com. ...

10. Technique: Probe Phage Genomes for Host Binding Proteins

    Type

    Blog Post

    ...will cleave DNA frequently enough to result in a smear of fragments that get smaller in size as a function...

11. Finding Your Perfect Job After University

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    Blog Post

    ...citizen, I was entitled to a Work-Travel VISA, which means I can gain employment for up to 6 months in Australia...

12. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type

    Blog Post

    ...should not be confined by birth country or financial means. Instead, talent should be realized through equal...

13. 9 tips for a successful postdoctoral experience

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    Blog Post

    ...and your development. Taken together, all of this means that you need to introduce yourself to and talk ...

14. When Fidelity Matters: A frank discussion about ligase fidelity

    Type

    Blog Post

    ...achieve exponential amplification. What does fidelity mean for a ligase? DNA ligases generally prefer fully...

15. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type

    Blog Post

    ...as their selection marker (Kim et al., 2014). Meanwhile Andy Fire’s lab used oligo-mediated knock-in of...

16. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type

    Blog Post

    ...plasmid amplification (Fischer, KB. 2019). This means that all DIO/FLEx AAV vectors will have some level...

17. Important Considerations When Using AAVs

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    Blog Post

    ...Modified from Aschauer et al. (2013) What does this mean to us? Well, an AAV user must determine which serotype...

18. Transferable Skills Guide: Teamwork

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    Blog Post

    ... all team members understand what roles they’re meant to fill and what their responsibilities are. That...

19. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type

    Blog Post

    ...the substrate with the target DNA sequence. This means cloning occurs in vivo, as compared to restriction...

20. Molecular Biology Reference

    Type

    Guide

    ...from a particular organism or group of organisms. Meaning, if a plasmid in intended for use in human cells...