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We narrowed to 941 results for: OMP;

Showing: 481 - 500 of 941 results

  1. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ... bulk of which you can find below. CRISPR for complex genetic diseases As CRISPR technology becomes increasingly...using it to investigate potential therapeutics for complex genetic diseases. So we’re revisiting this post...look at CRISPR’s potential for use in treating a complex genetic diseases, Alzhiemer’s disease (AD).  Troy...fibrosis (8).   The difference with CRISPR/Cas9 in comparison to other genome editing techniques is its ease...

  2. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    ... of the world was locked down, a South African company wanted to test if a type of antibodies from an ... power of collaboration, which despite the competitiveness of cutting-edge research, is still one of the...is done better with others than by oneself that prompted us to create Crowdfight.   Figure 1: Crowdfight...need help with your scientific research. It’s completely free, of course. You can find more information...

  3. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type
    Blog Post
    ... the vascular wall. ApoB, the main structural component of atherogenic lipoproteins, shuttles lipoproteins...Thus, the bEFPs bind the UAS via the dCas9-gRNA complex which allows transcription from the promoter.  ...should serve as both a powerful tool for generating complex regulatory systems and further the study of natural...hypothesizes that this may be due to different lipid compositions of C. elegans membranes, which might beneficially...

  4. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...liquid solution and pH paper to measure the pH. We compared the dry ice-stored samples to controls that were...ice for 72 hours and then vented the tubes by completely removing the caps, incubating the tubes in an...and then recapping the tubes prior to thaw. We compared vented and non-vented tubes to controls that were...various recovery periods between 0 and 96 hours. We compared these samples to controls that were only stored...

  5. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    ...cellular level. The reason for this is cost and complexity – anyone who works in the lab knows how expensive...expensive tiny amounts of reagents are, how complicated (and again, expensive) the different machines are...kits based on freeze-dried, cell-free (FD-CF) components. Making proteins outside of a cell - cell free..., feel free to check out one of our kits and accompanying curriculum to see if any of the activities fit...

  6. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...time. Both are helping researchers explore the complexity of translational control in cellular physiology... processing to avoid disrupting ribosome-mRNA complexes. Recent work has adapted this technique to monitor...color switching, TRICK requires the following components: a GFP protein that binds to the coding region... with mRNAs usually translated in a specific compartment. Despite these limitations, these techniques ...

  7. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...strand in Figure 1 below) was free to anneal to complementary ssDNA molecules in vitro. Figure 1: Cas9...discovered that single stranded donor DNA: Complementary to the released strand, 127bp in length, With...target DNA that was accessible to anneal with complementary single stranded DNA. We thus wondered if such... any of the error-prone repair that normally accompanies Cas9 cutting. We still don’t know the mechanism...

  8. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...Sharp. After a few rotations, Doench decided to complete his PhD work in Sharp’s lab.  Doench says, “what...what I loved about [RNAi], ... it was just this complete black box. The RNAi process had just been discovered...evolved into the Genetic Perturbation Platform and encompasses a variety of technologies to functionally investigate...he says, “In the case of CRISPR, the hype was completely justified… it has been just an incredibly useful...

  9. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ...guide for the quest of a perfect "fit". How competitive is the lab? Some labs have only one author plus...labs around the world. You may prefer a more competitive style to spur you to succeed or you might enjoy...enjoy working closely with others to accomplish your goals. You must understand your own style before making...Finding your dream lab While none of us will feel completely happy at work all of the time, the goal is to...

  10. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type
    Blog Post
    ...available. Plasmids are continually added as they complete QC and you can find all available plasmids listed...proteins, and membrane proteins. Although all viral components are available in bacterial expression plasmids...batches of samples. As soon as that process is complete, QC scientists prepare Sanger reactions by mixing...are met, we are taking a unique hybrid approach compared to our standard pipeline. For the first ~50 plasmids...

  11. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...being depicted, we can’t forget that those other components are still there in real life.  Certainly referring...interact with each other. Chromatin regulation is complex and influences--directly or indirectly--many other...PIs, articles, genes, and blog posts are not comprehensive, so just because you do not see a name or gene...phosphoinositol-dependent binding of the SWI/SNF-like BAF complex to chromatin after T lymphocyte receptor signaling...

  12. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    ...Konermann et al. Plasmids here How do Cas13d enzymes compare to other Cas13s? Like other Cas13 enzymes, the ...cleavage pattern is necessary. Konermann et al. compared CasRx to multiple RNA regulating methods: small... the clear winner with median knockdown of 96% compared to 65% for shRNA, 53% for CRISPRi, and 66-80% ...Addgene. Yan, Winston X., et al. “Cas13d Is a Compact RNA-Targeting Type VI CRISPR Effector Positively...

  13. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...library used. EvolvR: key components The EvolvR system has two key components: the error prone PolI3M that...variants in bacteria. Read on to learn about the key components of EvolvR and its potential applications. Challenges...interest. A gRNA is used to direct the PolI3M-nCas9 complex to a DNA site of interest, which nCas9 nicks and...

  14. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    ...to physically set up in the lab, RNAi is often completely ineffective or results in low penetrant phenotypes...membranes, organelles, and other subcellular compartments is via proteomics--the study of the distribution...low-cost, cell-free detection assay that can be completed without special equipment. What’s more, detection...encodes the LacZ𝛼 protein. Once expressed, LacZ𝛼 complexes with LacZ𝜔 and forms an active LacZ tetramer....

  15. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    ...controlling cell signaling. Key components of OptoNBs OptoNBs have two key components: an AsLOV2 domain and a ...activity in cells.  Let’s shed some light on the key components of OptoNBs, how they are generated, and their...variable heavy chain antibody domain.  How do these components interact? When fused together to form an OptoNB...

  16. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    ...chromosome. Targeted Insertion allows for direct comparisons between lines expressing different variants of...chromosome your transgene has integrated. This can be accomplished with a few matings to balancer chromosome lines...into a plasmid, you can send your plasmid to a company. For a few hundred dollars, they will inject the... your strategy and the services offered by the company, you might have to map into which chromosome the...

  17. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    ...No No Yes Yes Lab Made Competent Cells Required Yes No No No Comparison of REPLACR-mutagenesis with...mutations, large deletions/insertions require complicated, often costly in vitro assembly methods. A new...-mutagenesis also requires making your own electrocompetent cells in which the phage recombination machinery...

  18. All in a Twist: dsRNA

    Type
    Blog Post
    ...silencing complexes (RISCs), which detect dsRNA in the cytoplasm and use it as a guide to complementary RNA ...RNA sequence. Once a compatible sequence is found (typically RNA that the RNAi is designed against), RISC...and will dissociate when gene transcription is complete. However, transcription of thousands of genes ...

  19. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...camelid antibodies to antibody fragments to tools completely unrelated to antibodies.  Why use an alternative...entirely new affinity reagent.    Fig. 1: A comparison of an antibody (left) to several alternative ...through engineering instead of cleavage. They are comprised of the variable heavy and light chain regions,...immunogenic, but they have lower affinity and longevity compared to antibodies, along with a higher likelihood ...

  20. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ... the bulk purification steps keep the cost low compared to other antibody formats. This low barrier to...is no more of that exact polyclonal antibody composition available. If you’re going to need a large quantity...antibodies produce a higher level of background noise compared to monoclonal or recombinant antibodies. This ...polyclonal antibodies for your experiment and how they compare to other antibody options out there, you’re set...
Showing: 481 - 500 of 941 results