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We narrowed to 1,006 results for: RING

Showing: 481 - 500 of 1006 results

  1. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...Old Dominion University, who is majoring in marine biology and minoring in studio arts photography.  References...specific procedural instructions, including timing, preparing the smear, and mixing the stains and solutions.... & Walker S. The Bacterial Cell Envelope. Cold Spring Harb Perspect Biol. 2(5) a000414 (2010). Cleveland...

  2. Plasmids 101: NGS Quality Control for Pooled Libraries

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    Blog Post
    ...some of Addgene’s most exciting and versatile offerings! We recently re-amplified our distribution stock...sequencing. Maintain representation of the library during transformation First, use enough DNA and competent... a file in FASTQ format, a standard format for storing NGS reads and their corresponding sequencing quality...making sure few/no plasmids have dropped out, and comparing pre- and post-amplification sequencing results...

  3. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...S.H., Varmus H.E. Retroviruses. Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 1997. Principles...ITRs are perfectly symmetrical to allow for this pairing to occur. Like LTRs, the ITRs define the limit ... supplied by the production cell line (HEK-293) during production. Fun fact: There are 57 human adenovirus...

  4. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...expanded for multiplexed imaging for advanced monitoring of different factors, in different or the same...stimuli and as well as changes in cellular dynamics during so-called housekeeping activities.     ...cell (sub)types for expression and downstream monitoring. Nervous about making a virus for your biosensor...Biosensors for Neurotransmission and Neuromodulation: Engineering and Applications. Frontiers in Cellular Neuroscience...

  5. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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    Blog Post
    ... adenine base editor Lower-stringency PANCE, followed by higher stringency PACE, resulted in the evolution...which results in less overall dilution and less stringent phage selection. Figure 2: Phage-assisted...base editors? Targeted evolution of naturally occurring enzymes and functional domains speed up the development...

  6. 15 Years of Addgene: The Top 15 Plasmids

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    Blog Post
    ...for supporting scientific sharing. We’re very excited for more reagent sharing projects in the years to...15 years of plasmid sharing has certainly taken us on many adventures...From moving office locations ...Cas9 (SpCas9) has been widely popular in genome engineering. For use in human cell lines, the Zhang lab codon-optimized...

  7. Antibodies 101: Isotypes

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    Blog Post
    ...immune response. There are three main pathogen clearing functions or effector functions of the constant...each of the isotypes. IgM This isotype is produced during a primary immune response because all B cells begin...found in tissues. IgG is the predominant isotype during a secondary immune response, which is when the ...constant domains. This switching helps B cells adapt during the course of an immune response since it also ...

  8. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    ...milk processing, fruit juice clarification, meat tendering, and oligosaccharide synthesis. A major incentive...insoluble granules. We overcame this problem by engineering a plasmid which contained cold tolerant chaperones...editing for psychrophilic systems as well as engineering and regulating psychrophilic recombinant proteins...synthetic biology experiment Read our Genome Engineering Guide ...

  9. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...advantage to using the CRISPR/Cas system for genome engineering is the fact that Cas9 can be easily programmed...initial cut, the next steps in the process involve repairing chromosomal DSBs. It is important to know that...then ligates compatible DNA ends together, thus repairing the break. This is a simplified, streamlined ... ligation step determines how ends are resolved during nonhomologous end joining. Nat Commun 5: . https...

  10. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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    Blog Post
    ...rely on genetic engineering to optimize humans for CRISPRi and CRISPRa.” Before considering the use of pooled...that they could be used by people with little bioengineering expertise? “This is the next step in high-throughput...activating any human gene, go to http://sam.genome-engineering.org/. Crystal structure of Cas9 in complex with...

  11. Deep Dive: qPCR

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    Blog Post
    ...to double-stranded, and finally, drop once more during a final temperature increase. If there is a single...should only be one peak, as everything should be denaturing or annealing at the same temperatures. If there.... Flipping your experimental and control values during analysis can allow you to see this fold change ... vol. 1160, DOI 10.1007/978-1-4939-0733-5_3, © Springer Science+Business Media New York 2014Bustin S; ... as Reference for qPCR Gene Expression Analysis During iPS Reprogramming Process. Sci Rep 8, 8716 (2018...

  12. Viral Vectors 101: An Introduction to AAV

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    Blog Post
    ...vector into the culture medium during adeno-associated virus manufacturing." Human gene therapy 21.10 (2010...researchers speak about AAVs, they are actually referring to rAAVs. ITRs and serotypes The names of AAVs...different ages." Retinal Degenerative Diseases. Springer International Publishing, 2016. 501-507. PubMed...

  13. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...editors at Addgene. Levy et al., Nature Biomedical Engineering, 2020. PubMed PMID: 31937940. Detection of neural... prosthetic group PCB, PhyB and PIF6 dimerize, bringing each terminus of Cre together to restore Cre activity...to red light induces PhyBΔ and PIF6APB binding, bringing the two halves of Cre together. Image from Eisenhoffer...as well as in different stages of development, ensuring the breadth and versatility of this tool. Find...

  14. What's New in CRISPR - November 2021

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    Blog Post
    ... system. The CasMINI system was developed by engineering the Cas12f scaffold gRNA and making strategic...the gRNA scaffold through five rounds of gRNA engineering.  They did not modify the Cas12f1 enzyme. The...cargo RNA, and fusogen in a modular system for delivering gene editing tools into cells. Image adapted ... within the FAH gene was precisely corrected, restoring expression in the liver.  Read the paper in Nature...

  15. All in a Twist: dsRNA

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    Blog Post
    ...are typically contained within the mitochondria, barring a loss of mitochondrial integrity that would release...stranded structure produced is known as an R-loop. During transcription, RNA is synthesized from a DNA template...transcription of thousands of genes is constantly occurring throughout a single cell, so these nucleic acid...DNA hybrids Persistent RNA:DNA hybrids that arise during transcription are typically recognized by RNase...

  16. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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    Blog Post
    ... Mark Prescott. This system was developed for monitoring and analyzing autophagy of cytosol and organelles.... Autophagy is then induced and quantified by measuring the amount of radioactivity in the culture supernatant...autophagy markers. It’s particularly useful for comparing bulk autophagy vs. mitophagy, the targeted autophagy...Cuervo, A. M., & Seglen, P. O. (2007). Methods for Monitoring Autophagy from Yeast to Human. Autophagy,3(3)...

  17. CRISPRainbow and Genome Visualization

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    Blog Post
    ...with the previously established physical map. In comparing pairs of targets ~2 Mbp apart, they noticed that... magenta, depending on the hairpin combination bringing the total number of individual colors to 6. A ...Addgene. 4. Kiani, Samira, et al. “Cas9 gRNA engineering for genome editing, activation and repression... Mary Gearing...

  18. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    ...effects” have included using a Cas9 nickase variant, lowering Cas9 expression, and truncating gRNA sequences...decrease the off target effects of CRISPR/Cas9 by altering the nuclease activity of the Cas9 nuclease itself...excited to see continued improvements in genome engineering! References 1. Slaymaker, Ian M., et al. "Rationally...Plasmids Browse our CRISPR Guide Pages Find Genome Engineering Resources ...

  19. New Tool for Lineage Tracing: The ClonTracer Library

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    Blog Post
    ... populations resistant to drug treatments arise during treatment or are preexisting in the parent population... drug treatment and that these are selected for during the treatment process. The authors went on to show... “I spend a lot of time packing, shipping, and sharing protocols one by one,” and she is excited to deposit...Addgene because it is a “really great way of broadly sharing these kinds of reagents.” Here at Addgene, we’re...

  20. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...infrastructure are not always as effective in mentoring for this transition. Second, a coach will help...through on the actions you committed to. Finally, hiring a paid coach will make you take the experience ...Joanne's Reddit AMA I am a strong advocate for peer mentoring, but volunteer mentors are only able to go so ...areas of specialty from leadership development to mentoring for women and under-represented minorities. Her...
Showing: 481 - 500 of 1006 results