We narrowed to 601 results for: Ret;

1. Plasmids 101: Terminators and PolyA signals

   Type

   Blog Post

   ...Enhances Expression of Transgenes  Delivered by Retroviral Vectors. Journal of Virology. 1999. PubMed PMID...

2. The Materials Science of Optogenetics Experiments

   Type

   Blog Post

   ...inconsistent, and behavioral results impossible to interpret. Polishing the fiber optics with diamond polishing...

3. Experimenting in an Open Source lab: from CRISPR to Cats

   Type

   Blog Post

   ...Microsoft Research working on speech recognition, he returned to school to merge his interests in biology and...

4. The Scientific Conference Poster Session: Tips for Success

   Type

   Blog Post

   ...your poster on your computer screen than it is to pretend you’re standing at the session. Present with confidence...

5. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

   Type

   Blog Post

   ...With a few extra lines of code, we enabled it to return guides and primers in batch, optimized for HDR....

6. Polymerase Chain Reaction Overview and Applications

   Type

   Blog Post

   ...not as quantitative as other methods of PCR. Theoretically scientists should be able to determine the quantity...

7. Plasmids 101: Degron Tags

   Type

   Blog Post

   ...addition of a small molecule, and protein levels can return to normal once the drug has been removed. The degradation...

8. Degrading DNA with Cascade-Cas3

   Type

   Blog Post

   ...., Terns, M. P., White, M. F., Yakunin, A. F., Garrett, R. A., Van Der Oost, J., . . . Koonin, E. V. (...

9. 3D Printing Meets CRISPR Cas9

   Type

   Blog Post

   ... is docked on the membrane of the endoplasmic reticulum. And graduate students, momentarily forgetting...

10. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type

    Blog Post

    ...of normal protein function, and ultimately misinterpretation of the protein’s cellular role. One way to...

11. Viral Vectors 101: Producing Your rAAV

    Type

    Blog Post

    ...Golde, T. E. (2020). Utilizing minimally purified secreted rAAV for rapid and cost-effective manipulation...

12. Transferable Skills Guide: Managing a Team

    Type

    Blog Post

    ... realized that at the time I could likely have retained this talented undergrad. The point here is managing...

13. Google Forums Round Up: First Impressions of NgAgo

    Type

    Blog Post

    ...NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN RETRACTED AND FOLLOW UP STUDIES HAVE FAILED TO DEMONSTRATE...

14. Plasmids 101: Optimizing Plasmid Yields

    Type

    Blog Post

    ... in a protein expression strain, we suggest retransforming the plasmid into a strain meant for DNA purification...

15. Experimenting with New Careers while in Grad School

    Type

    Blog Post

    ...a lot of attention on social media. I gained a concrete example to use to promote future attempts and ...

16. PITChing MMEJ as an Alternative Route for Gene Editing

    Type

    Blog Post

    ... the region between the microhomology and the retention of a single microhomology sequence. For more mechanistic...

17. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type

    Blog Post

    ...easy Very hard Viral delivery   Lentivirus Retrovirus AAV Cells Dividing or nondividing Dividing ...

18. Genome-wide Screening Using CRISPR

    Type

    Blog Post

    ... to skewed results. Selecting a cell type - Theoretically, any cell type can be used in a CRISPR screen...

19. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type

    Blog Post

    ...recently to explore their use in plants, including FRET tools, double dimerization GFPs, and circular permutation...

20. Tips for Using BLAST to Verify Plasmids

    Type

    Blog Post

    ...This option is not as fast as megablast, but can return longer alignments to compare with your sequencing...