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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 613 results for: SET

Showing: 481 - 500 of 613 results

  1. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...courtesy of IPI. IPI anti-integrin toolset IPI’s anti-integrin toolset comprises 26 recombinant antibodies... that bound an amino acid region displayed by a subset of the RGD-binding integrins containing the αV ...

  3. Gamma-Retroviral Vector Guide

    Type
    Guide
    ... vectors that can be safely used in laboratory settings. These engineered gamma-retroviral vectors have...safety when producing viral vectors in a laboratory setting. Figure 1: Wild-type gamma-retrovirus genome. Created...DNA. However, if the plasmid contains a Gateway cassette containing the ccdB gene, a ccdB-resistant strain...

  4. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...responses to viral infections have resulted in the onset of cancer. Similar patterns of cancer development...inflammatory bowel disease is associated with the onset of colorectal cancer. Strategic immune evasion mechanisms...cytotoxic effector T cells, and targeting specific DC subsets in vivo using specific antigens to activate them...

  5. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ...Experiments by Jennifer Tsang Chromoproteins are a subset of the fluorescent protein family and they generate...molecular biology tools out there! Here, we highlight a subset of these tools for plasmid mapping, DNA/protein...

  7. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    ... (a specific mutation in a known gene = disease onset) may be best served by this type of targeted design...hits’ - genes that affect disease progression or onset. These hits can reveal individual genes or sometimes...

  8. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ...recycle our discards according to the Cambridge, Massachusetts guidelines and wear an extra sweater so we don... for smaller and smaller gel apparatus until I settled on using glass slides. With just the right amount...

  10. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...up positive traits (i.e. the plasmid contains a cassette that will make cells resistant to a toxin), whereas... a negative trait (i.e. the plasmid contains a cassette that will make cells sensitive to a toxin). In...

  12. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...career, I’d have realized it was a great fit for my skillset and interests. As such, I want to be able to share...re building software will help you be a valuable asset to the scientists as well as the developers. Lastly...

  13. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...found to have a point mutation in the puromycin cassette that makes it less efficient in some cell lines... It contains a point mutation in the puromycin cassette that makes it less efficient in some cell lines...

  14. Hot Plasmids - May 2022

    Type
    Blog Post
    ...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...proximity to the GFP-tagged protein (albeit slightly offset due to the nanobody-GFP interaction). The modularity...

  15. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...RNAs (gRNAs) are commonly packaged in 400-500 bp cassettes containing the RNA pol III promoter, gRNA and ...and pol III terminator. These relatively large cassettes (considering the gRNA itself is ~100 bases) limit...

  16. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...then subtracted from Ramanathan et al.’s RaPID datasets. Applications of RaPID RaPID sounds like a nifty...characterized by increased iron serum levels and early-onset cataracts, is characterized by point mutations in...

  18. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...excision or insertion, inversion, translocation or cassette exchange. By utilizing recombinases to manipulate...

  19. Hot Plasmids - October 2020

    Type
    Blog Post
    ...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ... hybrid gRNA library deletes human alternative cassette exons and disrupts genes with alternative exons...

  20. Hot Plasmids February 2024

    Type
    Blog Post
    ...COX8a-mStayGold. Scale bars 10 μm (main), 1 μm (inset). Image reused from Ando et al. 2023, under CC-BY...in young macaques and vascular bias in mature marmosets. CAP-Mac also robustly expressed fluorescent reporters...
Showing: 481 - 500 of 613 results