We narrowed to 605 results for: She

1. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

   Type

   Blog Post

   ...attention on transcription factors, starting with an established method called ChIP-seq. ChIP-seq relies on antibodies...

2. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

   Type

   Blog Post

   ...Hijacking tRNA processing to make gRNAs In a recently published paper, Yinong Yang’s group did just that as part...

3. How to Make Friends and Meet People at a Scientific Conference

   Type

   Blog Post

   ...Congratulate your connections when they get a new paper published. Stay in touch enough to plan to meet up at the...

4. Kiran Musunuru on the Newest TALEN Genome-Editing System

   Type

   Blog Post

   ...moment you are designing a TALEN on paper to a finished plasmid. With our protocol, it is feasible to ...

5. Ligation Independent Cloning

   Type

   Protocol

   ...of T4 Pol in the following step. This may be accomplished by gel purification . Step 5: Create Insert ...

6. Centrifugation

   Type

   Protocol

   ...centrifuge and investigate the issue. When you are finished spinning your samples, carefully remove them from...

7. Lentiviral Vector Guide

   Type

   Guide

   ... you're new to viral vectors or simply need a refresher, we've included a glossary at the end! Browse ...

8. Lighting Up Cell Signaling with Photoswitchable Kinases

   Type

   Blog Post

   .... Zhou, Xin X., Linlin Z. Fan, Pengpeng Li, Kang Shen, and Michael Z. Lin. "Optical control of cell signaling...

9. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

   Type

   Blog Post

   ...Oligomerization Seeing Red: Simple GFP Photoconversion Light Sheet Fluorescence Microscopy Additional Resources on...

10. CRISPRainbow and Genome Visualization

    Type

    Blog Post

    ...fluorescent distances correlating with the previously established physical map. In comparing pairs of targets ~...

11. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type

    Blog Post

    ...Addgene blogpost and more recently in a paper published in F1000Research on November 2016. Due to the ...

12. Antibodies 101: Selecting the Right Antibody

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    Blog Post

    ...and therefore each target protein. This can be accomplished by using a panel of primary antibodies conjugated...

13. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type

    Blog Post

    ...using blue light (Bubeck et al., 2018). They accomplished this by inserting a photo-sensitive LOV2 domain...

14. Antibodies 101: Conjugation

    Type

    Blog Post

    ...conjugated antibodies that are ready to use off the shelf. So why are we talking about this? Even though there...

15. Viral Vectors 101: An Introduction to AAV

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    Blog Post

    ...Serotype refers to the type of capsid (outer protein shell) used to package an rAAV vector; these capsids allow...

16. Transferable Skills Guide: Public Speaking

    Type

    Blog Post

    ...Your video conferencing software freezes and then crashes. You’re muted on Zoom again.  These things don’...

17. The time and cost required to make a plasmid

    Type

    Blog Post

    ... PI or corresponding author of the paper it’s published in. That’s also a great way to get to know people...

18. To Each HIS Own

    Type

    Blog Post

    ...used for a variety of assays in the lab (for a refresher see Antibodies 101: Introduction to Antibodies...

19. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type

    Blog Post

    ...PubMed PMID: 29553576. 2. Kittleson, Joshua T., Sherine Cheung, and JChristopher Anderson. "Rapid optimization...

20. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type

    Blog Post

    ...purpose. Tang wondered if he could use previously established mouse lines expressing GFP in specific cell types...