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TypeBlog Post...Biology with BioBuilder 3D Models for learning about CRISPR Resources at Addgene.org Find plasmids from the...
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Adeno Associated Virus (AAV) for Cell and Gene Therapy
TypeBlog Post...our Viral Vectors Featured Topic Page Learn about CRISPR-AAV vectors Learn about Addgene AAV Quality Control... -
Hot Plasmids February 2024
TypeBlog Post...cells By Susanna Stroik Even with the advances in CRISPR technology over the past decade, primary cells ... -
Viral Vectors 101: Calcium Sensors
TypeBlog Post...with genetically encoded calcium indicators. Cold Spring Harbor Protocols. 2012 Jun 1;2012(6):pdb-top069609... -
Early Career Researcher Toolbox: Finding Relevant Papers
TypeBlog Post...including a bot for biosensor papers, microbe articles, CRISPR papers, and more. Saving papers There isn’t technically... -
5 Tips for Troubleshooting Viral Transductions
TypeBlog Post...adoptive immunotherapy." Acute Leukemias VIII. Springer Berlin Heidelberg, 2001. 291-306. PubMed PMID:... -
Tips for Titering Your Lentiviral Preps
TypeBlog Post... Lentivirus FAQ Learn How Lentivirus is Used in CRISPR Genome-Wide Screening Learn How Lentivirus Has ... -
Chromoproteins: Colorful Proteins For Molecular Biology Experiments
TypeBlog Post...red color can be used for mimicry. Image: Bernard Spragg. Advantages of using chromoproteins for molecular... -
Plasmids 101: 5 factors to help you choose the right cloning method
TypeBlog Post.... You’re going to engineer the best thing since CRISPR; you are ready to clone! But...how? Cloning is... -
Polymerase Chain Reaction Overview and Applications
TypeBlog Post...identified in the late 1960s during research at hot springs in Yellowstone National Park. In addition to Taq... -
Hot Plasmids: Winter 2025
TypeBlog Post...to dicot species. In addition, they generated offspring tomato plants that inherited the genomic edit ... -
Common Injection Routes in Mice
TypeBlog Post...resources on Addgene.org Genome Engineering Guide CRISPR Mammalian Expression plasmids Cre-Lox plasmids ... -
Antibodies 101: Conventional vs Spectral Flow Cytometry
TypeBlog Post...t “wash out” the rest of the panel and create spreading error. Conversely, markers with low expression... -
Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity
TypeBlog Post...illustrates how the volume of the 1 ml outgrowth spread on each transformation plate can be manipulated... -
Addgene's Cancer Collection Pages Connect the Research Community
TypeBlog Post... infiltrate the circulatory or lymph systems to spread, or metastasize. Many cancerous cells will die ... -
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog Post...others at the Sainsbury are working on a lot of CRISPR tools for editing plant genomes. We will definitely... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog Post...Analysis of Protein Interactions in Live Cells. Cold Spring Harb Protoc 2013:pdb.prot076497. https://doi.org... -
A Guide to Starting Your Own Journal Club
TypeBlog Post... a week for several months at a time during the spring and fall semesters. Our scheduling was in part ... -
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog Post...Immunofluorescence Staining. Methods in Molecular Biology. Springer New York, pp 299–311. https://doi.org/10.1007/... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog Post...Vectors for Delivery of Gene-Editing Systems Based on CRISPR/Cas: Current State and Perspectives. Viruses, 13...