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...Addgene has distributed over 100,000 CRISPR plasmids. But that’s not our only job - we strive to also give...

...able to understand what metrics would be useful and how to organize the metrics in a digestible manner....

... functions they may induce?  Fc receptors are intriguing from a therapeutic standpoint. Engineered monoclonal.... (2008). The immunoglobulin constant region contributes to affinity and specificity. Trends in Immunology...

...Addgenie Mary Gearing contributed to the content of this article. Biosensors (‘biological sensors’) are...separated by CaM and M13. When calcium binds, it triggers CaM/M13 interaction that increases FRET transfer...

... tool to detect and image DA in live animals (Patriarchi et al., 2018). Similar to calcium and glutamate...PMID: 4403305. PubMed Central PMCID: PMC426888. Patriarchi, Tommaso, et al. "Ultrafast neuronal imaging ...

...    sdAbs in research These advantages have contributed to sdAbs becoming valuable tools for diverse ... approved in 2018, with many others in clinical trials (Jin, et al. 2023). In addition to therapeutics...

...for sale, and photos of the Agar Art competition entries were displayed in a gallery on the second floor... to find out more about our free deposit and distribution service! Did you see a must-have plasmid during...

...RFP, no FP). The vectors each contain two SfiI restriction sites with different overhangs depending upon...Cancer Center presented results from a clinical trial using the Sleeping Beauty transposase as a nonviral...

...entire meeting, Monday's talks alone were worth the trip. Dr. Dana Carroll's excellent keynote address was...genome engineering tools – both deposited and distributed – have changed so dramatically in the past 4 ...

...one step involves boiling the glass powder in 50% nitric acid, but I never let that stop me. I only had ...glass slide using only surface tension.  It's a bit tricky to pour the right amount so the agarose doesn’t...

...to the antibiotic that remains intact. Tips and tricks from the bench Use fresh stocks. Most antibiotics... Marcy Patrick...

...fantastic fodder for science communication. The trick is to find one or two roadblocks that sparked creativity...makes them want to know more. This could be by outright stating the big-picture problem, by asking your...

...mL Amp instead of 100 μg/mL Amp), or using more nutrient rich media can improve growth and DNA yield. Test...determine if your sample has recombined. Choose a restriction enzyme that will give you different band patterns...

...This post was contribued by guest bloggers Aline and Benjamin Glick from SnapGene. SnapGene was created...don’t use SnapGene yet, you can download a free trial. If you notice that a feature is missing from our...

... This post was contributed by guest blogger Jack Leeming Editor of Naturejobs, part of Nature magazine...dialogue specifically about the project and contributions, rather than getting defensive or getting into...

...fragments that recognize the endogenous IgGs (Lu and Partridge, 1998). After washing away unbound fragments, ...https://doi.org/10.1369/0022155413511620  Lu QL, Partridge TA (1998). A New Blocking Method for Application...

...HEKs have an unusual ploidy and are considered hypotriploid: most chromosomes are represented by two to ...cells may be particularly useful due to their intrinsic properties. HEK-293 cells are tumorigenic, ...

...This post was contributed by Richi Sakaguchi from Kyoto University, and Marcus N. Leiwe and Takeshi Imai...copies per cell per color, following a Poisson distribution. To overcome the limited expression levels of...

... dark matter and so the lab organized a sampling trip to Yellowstone National Park (YNP). Extremophiles... another Quake Lab member joined for half of the trip. Preparations ahead of time included contacting ...

...suggest topics you’d like to see on the blog or to contribute ideas for your own guest posts by e-mailing us...generate knockouts, but how does it work? Guest contributors David Wyatt and Dale Ramsden from UNC Chapel...