...Podcast breakdown (Time : Topic) 0:00 - 2:00 : Introduction to the podcast from producers Tyler Ford and...and Eddy Page. 2:00 - 3:55 : Alex introduces himself and explains why he decided to do an MD PhD - it's...in generating Cas9 based tools to enable facile control of DNA and RNA.     Special thanks to our sound...

...ultimate prize – a glorious trophy. Sure it’s plastic and you have to share 1 trophy among 6 people, but you...occurring earlier but that the photo evidence was destroyed to protect the innocent revelers. Our Halloween...

...interview with Addgene's founders Podcast breakdown Intro Chapter 1 - Who are Addgene's Founders? Chapter ...the importance of sharing information/knowledge Outro Many thanks to our guests, Melina Fan, Kenneth ...nonprofit? Get an Inside Look at Addgene's NGS Quality Control Addgene's Viral Service - Why Virus? Why Now? ...

...AAV9 tdTomato virus in the mPFC.  The AAV9 is so strong that you can even see dendritic spines!  Curious...Images that clearly show viral spread and cellular tropism* (min. 300 dpi, PNG format) At least two replicates... the target region, if the report describes a retrograde serotype An abstract that summarizes your experiment...

...grant to order plasmids she can use as positive controls in PCR-based cervical cancer screens. This third...predominantly HPV 16 and 18.” HPV plasmids as positive controls To start her work, Gladys will improve cervical... containing a variety of HPV genes as positive controls in her PCR screens. She hopes that these screens...

...projects 0:28 - 1:12 : Introduction from Tyler 1:13 - 2:02 : Niroshi and Ben introduce themselves 2:03 - 3...your biggest experimental success? 31:32 - end : Outro In our next episode, Niroshi and Ben discuss how...

...the performance of different nucleases. Nicola Patron’s lab compared the efficiency and specificity of...plasmids Anti-CRISPR proteins for optogenetic control of CRISPR-Cas9 Anti-CRISPR proteins that inhibit...has adapted anti-CRISPR proteins for optogenetic control of CRISPR-Cas9. By combining AcrIIA4, a Streptococcus...

...They kicked off their day with breakfast and an introduction to Addgene with our Executive Director, Joanne...in-depth look at some of the teams at Addgene. An introduction to plasmids with Director of Biology Lianna ...our UK team member Benoit Giquel. Bluegene’s globetrotting impressed our guests, as did how much work is...

...parental cell line, but not in the iso-genic knockout control. This can involve cell surface labeling and/or ... Additionally, it can be a real challenge to troubleshoot RNAi failures. Potential explanations for RNAi... target of interest, you can add an additional control containing treated cells. The expected change in...55133-7 More resources from the Addgene blog Introduction to Gating in Flow Cytometry Antibodies 101: ...

...for use as a negative control, and empty backbones such as pBABE-puro for retroviral expression and pLVTHM...psPAX2. Actually, these two plasmids from Didier Trono's lab are our two most popular plasmids of the past...

... they do so by activating signaling pathways controlling properties like growth, survival, and differentiation...inactivated the pathway. “The idea is that if you introduce them into cells one at a time, you could identify... for pathways that drive resistance to drugs in vitro, and they’ve shown that it works. But, he says that...

...growth based on data voluntarily made available by Medtronic via The Yale University Open Data Access (YODA...got a nine percent bump in citations for their trouble, even when all kinds of other factors, including...universities. We particularly recommend Eric Lander's intro to biology course "The Secret of Life." 10)     ...

...for packaging: AAV1, AAV2, AAV5, AAV8, or AAV Retrograde (AAVrg) capsids.  Please be aware that this ordering...Packaged on Request prep and run the same quality control assays we run with all of our in-stock viral preps...plasmids packaged in AAV1, AAV5, AAV8, and AAV Retrograde to be 1x1013 and titers for AAV2 to be 7x1012...

...McClean’s lab generated an optogenetic toolkit that controls repression of gene expression in Saccharomyces...transcriptional repressor Mxi1, and an optogenetic tool that controls nuclear localization (LINuS). Blue light renders...mRNA decreased after 20 minutes in xylose (which controls expression of dCas9). The lab demonstrated the...

... system is toxic to proliferating cells. A Cre-estrogen receptor ligand binding domain-fusion can prevent... toxicity in proliferating Drosophila cells by estrogen-dependent activity regulation. Heidmann D, Lehner... BAC/PAC recombineering techniques. Semprini S, Troup TJ, Kotelevtseva N, King K, Davis JR, Mullins LJ...

... me like the postdocs are taking some steps to control their own future in science (see my previous blog...vehemently about the need for change.  Dr. Bourne strongly believes this is an amazing time of promise for... Novartis industry postdoctoral program.  His strongest comments were about the astounding lack of reproducibility...

...Getting into the office for the first time, being introduced to all the members around the office one by one...and undigested samples of the clones on an electrophoresis gel in order to differentiate which clone had...Once we had gotten our results back from the electrophoresis, my manager and I were unsure if there were...

...the workforce, but my main concerns were building strong connections with coworkers and doing something ...interested in knowing you as a person, builds a strong sense of trust between coworkers. Since I trusted...about making connections with coworkers seem, in retrospect, nonsensical. I have found the people at Addgene...

...and/or the use of chelating agents that can be introduced in the purification protocol to remove endonucleases... your preparation for a long time. Tris buffer controls the pH, while the EDTA chelates any divalent cations...of using a Tris buffer is that it allows better control of the pH preparation stabilizing the plasmid, ...

... sections of a paper, the abstract, figures, introduction, etc. so as to thoroughly explain the do’s and...reader is not in their head. The scientist has to introduce the reader to the manuscript and engage them in...to make the final product with an interesting introduction and concise discussion. Many thanks to our ...