Generating Mouse Models Using CRISPR/Cas9
Type
Blog Post
...the run, it got “dirty”, with multiple sequence traces overlapping each other! When the count was done...
We narrowed to 508 results for: Trac
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Building Global Connections with the International Mentorship Program USA-EUROPE
TypeBlog Post... am not sure that I want to pursue the academic track.” How does an International Mentorship Program work... -
9 tips for a successful postdoctoral experience
TypeBlog Post... and how important is the result? Is there a tractable question or are you simply describing a phenomenon... -
Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering
TypeBlog Post... a lambda red phage, λTetR, that carries the tetracycline resistance gene and the lambda red repressor... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog Post... single-nucleotide substitutions along a 1.5 kb tract, all without selectable markers. CRISPR in C. elegans... -
Finding Your Science Policy Path
TypeBlog Post...for skill development include leadership of extracurricular organizations, working in student government... -
Antibodies 101: Antibody Engineering and Directed Evolution
TypeBlog Post...Bioengineering is a field that has been gaining a lot of traction in recent years. The goal is to engineer the most... -
Which Fluorescence Microscopy Technique is Best for Me?
TypeBlog Post...sectioning. Optical sectioning refers to the ability to extract light from a single, thin plane within a relatively... -
Cancer and the Immune System: Deciphering the Relationship
TypeBlog Post...Check out Our Cancer Collection Pages Use the Clon Tracer Library to Research Cancer Drug Resistance Checkout... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog Post...Adeno-associated viral vectors for anterograde axonal tracing with fluorescent proteins in nontransgenic and ... -
CRISPR 101: Multiplex Expression of gRNAs
TypeBlog Post... separately inducible by L-arabinose and anhydrotetracycline (aTet), respectively. pMAZ-SK contains an... -
CRISPR 101: Cytosine Transversion Editors
TypeBlog Post...post! Here’s a quick refresher if you ever lose track of which acronym is which. Acronym Full name... -
A Needle in a Base-Stack: Cas9 Structural Biology
TypeBlog Post...with a guide RNA — whether it’s an sgRNA or a crRNA:tracrRNA duplex — Cas9’s two lobes move toward each ... -
Delivery Methods for Generating iPSCs
TypeBlog Post...EBNA1 plasmid to be maintained as a stable extrachromosomal piece of DNA. Reprogramming efficiency is ... -
Deep Dive: qPCR
TypeBlog Post...method, and other small variations in consumables. Track and record these details for reproducibility’s sake... -
15 Hot Plasmids from 2017
TypeBlog Post...discovered reprogramming discrepancies, which they traced to a 9 amino acid N-terminal variation in the Klf4... -
22 Hot Plasmid Technologies from 2014
TypeBlog Post...dimerize on exposure to light, bringing the intracellular kinase domains into contact and initiating signaling... -
Adeno-associated virus (AAV) Guide
TypeGuide...you to track neurons that send information to a given region. This allows researchers to trace connections...using AAV for neuronal tracing . Browse AAV vectors for monosynaptic neuronal tracing available at Addgene... particles produced in HEK293T cells are then extracted and purified for their use as viral vectors to...molecular weight structures that are maintained extrachromosomally. Random integration of rAAV genomes in non-homologous... capsid could avoid invasive methods such as intracranial injection. Systemic capsids are often derived... a specific brain region, AAVrg would enter and track the terminal axons present in that region and move...diseases such as cystic fibrosis and cancer. Neuronal Tracing Recombinant AAV vectors have been widely used by... -
Chemogenetics Guide
TypeGuide...important for dosing in animal studies, made them attractive targets for development into chemogenetic tools...stimulating phospholipase C, which releases intracellular calcium stores. There are currently three Gq...and activate neuronal signaling by increasing intracellular cAMP concentrations. There is currently one ... was created by replacing the corresponding intracellular region of a turkey erythrocyte β-adrenergic ...the same back-metabolism issues as CNO and are attractive alternatives for experiments. Perlapine has been...used in human populations in Japan, making it an attractive option for translational studies, however, it...be an agonist against hM4Di, and is especially attractive for use in translational studies because it is... -
Antibody Guide
TypeGuide...samples to extract proteins. Homogenize (tissues only), lyse, and then collect protein extract by centrifugation...and therefore should be kept at 4 °C. Record and track lot numbers for all antibodies used in the lab. ...application Process tissue or cell culture samples to extract proteins. Homogenize (tissues only) and lyse. Cross-link...In IHC, tissue sections are assayed and the extracellular matrix remains intact. The signaling molecule...enzyme. In ICC, cells have most or all of their extracellular matrix removed. Again, the signaling molecule...