... a lambda red phage, λTetR, that carries the tetracycline resistance gene and the lambda red repressor...

... and how important is the result? Is there a tractable question or are you simply describing a phenomenon...

...for skill development include leadership of extracurricular organizations, working in student government...

... single-nucleotide substitutions along a 1.5 kb tract, all without selectable markers.  CRISPR in C. elegans...

...sectioning. Optical sectioning refers to the ability to extract light from a single, thin plane within a relatively...

...Check out Our Cancer Collection Pages Use the Clon Tracer Library to Research Cancer Drug Resistance Checkout...

...Adeno-associated viral vectors for anterograde axonal tracing with fluorescent proteins in nontransgenic and ...

... separately inducible by L-arabinose and anhydrotetracycline (aTet), respectively. pMAZ-SK contains an...

...with a guide RNA — whether it’s an sgRNA or a crRNA:tracrRNA duplex — Cas9’s two lobes move toward each ...

...post! Here’s a quick refresher if you ever lose track of which acronym is which. Acronym Full name...

...EBNA1 plasmid to be maintained as a stable extrachromosomal piece of DNA. Reprogramming efficiency is ...

...method, and other small variations in consumables. Track and record these details for reproducibility’s sake...

...discovered reprogramming discrepancies, which they traced to a 9 amino acid N-terminal variation in the Klf4...

...dimerize on exposure to light, bringing the intracellular kinase domains into contact and initiating signaling...

...important for dosing in animal studies, made them attractive targets for development into chemogenetic tools...stimulating phospholipase C, which releases intracellular calcium stores. There are currently three Gq...and activate neuronal signaling by increasing intracellular cAMP concentrations. There is currently one ... was created by replacing the corresponding intracellular region of a turkey erythrocyte β-adrenergic ...the same back-metabolism issues as CNO and are attractive alternatives for experiments. Perlapine has been...used in human populations in Japan, making it an attractive option for translational studies, however, it...be an agonist against hM4Di, and is especially attractive for use in translational studies because it is...

...samples to extract proteins. Homogenize (tissues only), lyse, and then collect protein extract by centrifugation...and therefore should be kept at 4 °C. Record and track lot numbers for all antibodies used in the lab. ...application Process tissue or cell culture samples to extract proteins. Homogenize (tissues only) and lyse. Cross-link...In IHC, tissue sections are assayed and the extracellular matrix remains intact. The signaling molecule...enzyme. In ICC, cells have most or all of their extracellular matrix removed. Again, the signaling molecule...

...coined the term plasmid, in reference to any extrachromosomal heritable determinant. Plasmids are fragments...fragments can be inserted. When a plasmid exists extrachromosomally in E. coli , it is replicated independently...shaped bacteria naturally found in the intestinal tract of animals. There are many different naturally occurring.../mL 50 µg/mL Spectinomycin 50 mg/mL 50 µg/mL Tetracycline 10 mg/mL 10 µg/mL *Note: Carbenicillin can be...

...multiple shuttle vectors for different purposes. The pAdTrack series contains an IRES-GFP construct that enables...transgene of interest. With these plasmids, one can track the infection of 293/911 cells throughout virus ...based vector Most common type of adenoviral vector pAdTrack Class of transfer vectors that contain IRES-GFP...

...Functional Screens New Tool for Lineage Tracing: The ClonTracer Library...that can be used for applications like lineage tracing or parsing the effects of expressing multiple genes...

...dynamic tracking of repetitive and non-repetitive genomic loci, chromosome painting, and RNA tracking in living...editing can specify a single mutation, even within a tract of identical bases. Browse Plasmids: Prime Edit ...proteins that target RNA instead of DNA enables tracking of RNA in live cells, including orthogonal RNA...synthetic fusion of the endogenous bacterial crRNA and tracrRNA that provides both targeting specificity and scaffolding...