We narrowed to 749 results for: UTY

1. Generating Mouse Models Using CRISPR/Cas9

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   ...Wenning Qin and Haoyi Wang. CRISPR/Cas9 is revolutionizing the mouse gene-targeting field. Mice have long... he is interested in using CRISPR to develop therapeutic applications that better human health.     References...

2. When Fidelity Matters: A frank discussion about ligase fidelity

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   ...and circularization of dsDNA (6). Less commonly utilized in vitro, Taq DNA ligase will ligate only nicks...replacements for T4 DNA Ligase in applications such as routine cloning or DNA library preparation. However, when...

3. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

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   ...to generate a monoclonal cell lines by limiting dilution. The rest of the cells were frozen down. ...Isolating a Monoclonal Cell Population by Limiting Dilution Lentivirus Production Websites Primer3: for ...

4. RNA Interference in Plant Biology: New Tools for an Old Favorite

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   ...techniques It is fair to say that CRISPR has revolutionized molecular biology and has enabled functional.... Nevertheless, amiRNA-based RNAi experiments routinely display highly specific and robust gene silencing...

5. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

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   ...airflow before and while you are working. Perform routine checks for mycoplasma contamination, before using...importance of preventing contamination. Be aware and cautious rather than learning it the hard way! Many thanks...

6. Finding Your Science Policy Path

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   ...legislation (see Figure 1 for career map). The beautiful part of science policy is that you can jump from...work can happen at the national level within the executive branch or in Congress, or can also happen at the...

7. Custom CRISPR Screens & the Green Listed Software

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   ...reference library. If this happens to you, one solution is to identify all the alternative names for your...screen identifies genetic vulnerabilities and therapeutic targets in acute myeloid leukemia." Cell reports...

8. Special Delivery: Fluorophore Targeting for FRET Studies

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   ...that can be inserted into the target protein. The utility of these reagents is that binding is covalent, ...Observation of intersubunit movement of the ribosome in solution using FRET." Journal of molecular biology 370.3...

9. CRISPR 101: Cytosine Transversion Editors

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   ...The first base editors revolutionized CRISPR gene editing. Cytosine base editors (CBEs) and adenine base...than an accessible/inaccessible binary. A word of caution: CGBEs and indels Cytosine transversion editors...

10. Antibody Validation for Flow Cytometry

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    ...methanol, PFA-saponin, PFA-triton). The fix/perm solutions can make a significant difference in the efficacy...and methanol, we found that the optimal perm/fix solution depended on the specific antibody:target combination...

11. Thanks to Addgene Depositors, We’re Now Sharing over 100,000 Reagents!

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    ...excited to share some good news with you: with contributions from depositors around the globe, we’re now ...

12. Introducing the Addgene Antibodies 101 eBook!

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    ...easily access relevant information for planning, executing, and troubleshooting your experiments. For example...

13. New FLIS Plasmid (April Fools 2022)

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    ...and re-contain any specimens that escape during routine lab activities.  Here, we demonstrate the new system...

14. Lucky Thirteen: Guest Contributors of 2023

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    ...contributed to the Addgene blog this year! Your contributions have helped researchers around the globe find...

15. Overwhelmed? Take a Break With Our 5 Favorite Science Comics

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    ...) ourselves back into our regular post-holiday routines, why not take a little time to appreciate the ...

16. New Videos: Addgene Lab Tips

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    ...several times. This will help ensure ensure equal distribution. Lab tip #3: Barcode everything Use barcodes...

17. Find and Share AAV Data with Addgene's New AAV Data Hub

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    ...the data hub website as a thank you for your contribution to open science.  Please note that the AAV Data...

18. DIY DNA Ladders from Penn State University

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    ...Smart Phone Apps for Lab Life Learn About the Evolution of Lab Techniques Resources on Addgene.org Use...

19. Twelve Amazing Guest Blog Contributors!

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    ...Great Depositor Research Stories Interested in contributing to the Addgene blog? We'd love to hear from ...

20. Introducing Addgene's Microbiology Pages

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    ... a colleague who would benefit from our free distribution services for their popular constructs, why not...