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  2. Optogenetics Guide

    Type
    Guide
    ...for light-induced cAMP modulation. Utilizes BLUF (blue light utilizing FAD) domains. 441 Phytochromes BphP1...technologies developed for optogenetics research utilize light to detect, measure, and control molecular...expressed under the control of the vesicular gamma aminobutyric acid (GABA) transporter (VGAT) and ChR2 is thus...figure above, this light induced binding can be utilized to control the localization of a protein of interest...of neural activity, with single-spike temporal resolution. PLoS One. 2(3):e299. PMID 17375185 Kim B, Lin...

  3. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...reference library. If this happens to you, one solution is to identify all the alternative names for your...screen identifies genetic vulnerabilities and therapeutic targets in acute myeloid leukemia." Cell reports...

  4. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...that can be inserted into the target protein. The utility of these reagents is that binding is covalent, ...Observation of intersubunit movement of the ribosome in solution using FRET." Journal of molecular biology 370.3...

  5. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...The first base editors revolutionized CRISPR gene editing. Cytosine base editors (CBEs) and adenine base...than an accessible/inaccessible binary. A word of caution: CGBEs and indels Cytosine transversion editors...

  6. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...methanol, PFA-saponin, PFA-triton). The fix/perm solutions can make a significant difference in the efficacy...and methanol, we found that the optimal perm/fix solution depended on the specific antibody:target combination...

  7. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...Aug;10(2):302-17. PubMed . From virus evolution to vector revolution: use of naturally occurring serotypes...thus thought to be the most suitable virus for therapeutic applications. Clinical trials using AAV for various...gene delivery vectors: molecular engineering and evolution of adeno-associated viral vectors for enhanced...

  8. Lentiviral Guide

    Type
    Guide
    ...transfer plasmid. 2nd generation lentiviral plasmids utilize the viral LTR promoter for gene expression, whereas...whereas 3rd-generation transfer plasmids utilize a hybrid LTR promoter (more information on this below)...office can provide more information on your institution's best practices with regard to lentiviral research...generation vectors use a hybrid 5' LTR with a constitutive promoter such as CMV or RSV. 3' LTR in cis Terminates...

  9. Chemogenetics Guide

    Type
    Guide
    ...vivo and the receptors exhibited high levels of constitutive activity, making them less than ideal. DREADDs...insensitivity to endogenous ligands, have low constitutive activity, and their activating ligands have ...This DREADD was shown to have a small amount of constitutive activity, and is not widely used. There is also...cross the blood-brain barrier in rats, and the utility of DREADDs in experiments in this species may be...

  10. Molecular Biology Reference

    Type
    Guide
    ... DNA sequencing of the plasmid. Primers can be utilized to verify the sequence of the insert or other ...ampicillin. Preparing Antibiotics Create a stock solution of your antibiotic. Unless otherwise indicated...dH 2 0. Addgene recommends making 1000X stock solutions and storing aliquots at -20°C. To use, dilute ..., like Sanger, is based on DNA replication and utilizes modified fluorescently tagged nucleotides. During...

  16. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ...several times. This will help ensure ensure equal distribution. Lab tip #3: Barcode everything Use barcodes...
Showing: 481 - 500 of 739 results