We narrowed to 752 results for: UTY;

...Wenning Qin and Haoyi Wang. CRISPR/Cas9 is revolutionizing the mouse gene-targeting field. Mice have long... he is interested in using CRISPR to develop therapeutic applications that better human health.     References...

...techniques It is fair to say that CRISPR has revolutionized molecular biology and has enabled functional.... Nevertheless, amiRNA-based RNAi experiments routinely display highly specific and robust gene silencing...

...airflow before and while you are working. Perform routine checks for mycoplasma contamination, before using...importance of preventing contamination. Be aware and cautious rather than learning it the hard way! Many thanks...

...legislation (see Figure 1 for career map). The beautiful part of science policy is that you can jump from...work can happen at the national level within the executive branch or in Congress, or can also happen at the...

...and circularization of dsDNA (6). Less commonly utilized in vitro, Taq DNA ligase will ligate only nicks...replacements for T4 DNA Ligase in applications such as routine cloning or DNA library preparation. However, when...

...to generate a monoclonal cell lines by limiting dilution. The rest of the cells were frozen down. ...Isolating a Monoclonal Cell Population by Limiting Dilution Lentivirus Production Websites Primer3: for ...

...reference library. If this happens to you, one solution is to identify all the alternative names for your...screen identifies genetic vulnerabilities and therapeutic targets in acute myeloid leukemia." Cell reports...

...that can be inserted into the target protein. The utility of these reagents is that binding is covalent, ...Observation of intersubunit movement of the ribosome in solution using FRET." Journal of molecular biology 370.3...

...The first base editors revolutionized CRISPR gene editing. Cytosine base editors (CBEs) and adenine base...than an accessible/inaccessible binary. A word of caution: CGBEs and indels Cytosine transversion editors...

...methanol, PFA-saponin, PFA-triton). The fix/perm solutions can make a significant difference in the efficacy...and methanol, we found that the optimal perm/fix solution depended on the specific antibody:target combination...

...excited to share some good news with you: with contributions from depositors around the globe, we’re now ...

...easily access relevant information for planning, executing, and troubleshooting your experiments. For example...

...contributed to the Addgene blog this year! Your contributions have helped researchers around the globe find...

...and re-contain any specimens that escape during routine lab activities.  Here, we demonstrate the new system...

...) ourselves back into our regular post-holiday routines, why not take a little time to appreciate the ...

...several times. This will help ensure ensure equal distribution. Lab tip #3: Barcode everything Use barcodes...

...the data hub website as a thank you for your contribution to open science.  Please note that the AAV Data...

...Great Depositor Research Stories Interested in contributing to the Addgene blog? We'd love to hear from ...

...Smart Phone Apps for Lab Life Learn About the Evolution of Lab Techniques Resources on Addgene.org Use...

... a colleague who would benefit from our free distribution services for their popular constructs, why not...