We narrowed to 577 results for: Vit

1. Corralling and Cornering the Goldilocks Antibody

   Type

   Blog Post

   ...underlying causes of this unreliability: cross-reactivity (binds to non-target proteins), variability (...

2. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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   Blog Post

   ...quantitative readout of the interaction in vivo or in vitro, and can be used for measuring protein expression...

3. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

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   Blog Post

   ...25653845. Pubmed Central PMCID: PMC4304227. 4. Revitalizing biomedical research: recommendations from the...

4. 5 Tips for Troubleshooting Viral Transductions

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   Blog Post

   ...given viral stock will give you a sense of the infectivity of your viral stock, save you time, and allow...

5. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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   Blog Post

   ...Canton Nucleic Acids Forum (CNAF) 2015 conference invited me to attend and speak about Addgene’s experience...

6. How-to: 5 Steps to a Great Panel Discussion

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   Blog Post

   ...incorporate them into the program. I have to admit to inviting interesting friends and colleagues to events for...

7. Plasmids 101: 5 factors to help you choose the right cloning method

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   Blog Post

   ...give Gateway a try. This method, developed by Invitrogen and based on lambda phage integration sequences...

8. Plasmids 101: Protein Expression

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   Blog Post

   ...cell-free expression systems, proteins are assembled in vitro using purified components of the transcription and...

9. AAV Vector Quality Control: Going the Extra Mile with NGS

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   Blog Post

   ...contamination is a good start, but to determine the sensitivity of our method we wanted to see how low we could...

10. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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    Blog Post

    ...TRiP- Functional Genomics Resources website has in vitro reagents and tools to help you knock down, knock...

11. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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    Blog Post

    ...useful for your next localization experiment and invite you to make any fluorescent protein constructs ...

12. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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    Blog Post

    ...for trimethoprim, there would be no changes in sensitivity. Pooled knockdowns are also possible with Mobile-CRISPRi...

13. Tips for a 1st time AAV user (by a Rookie AAV user)

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    Blog Post

    ...recombinant adeno-associated viral vectors for in vitro and in vivo use. Current protocols in molecular ...

14. PhD Applications After COVID

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    Blog Post

    ... receiving applications, universities typically invite a select group of students to continue the application...

15. Production of Virus in Insect Versus Mammalian Cells

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    Blog Post

    ...insectspodoptera frugiperda (lepidoptera; noctuidae). In Vitro, 13(4), 213–217. https://doi.org/10.1007/bf02615077...

16. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type

    Blog Post

    ...Jung, D., Yoon, S., & Kim, H. H. (2019). SpCas9 activity prediction by DeepSpCas9, a deep learning–based...

17. Twenty Years of Addgene Sharing: CRISPR

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    Blog Post

    ...of new tools, illustrated in Figure 2, and the longevity of tools seen in Figure 3, indicates that the ...

18. Polymerase Chain Reaction Overview and Applications

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    Blog Post

    ...a polymerase chain reaction (PCR). PCR is an in vitro method in which a small amount of DNA can be copied...

19. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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    Blog Post

    ... Doench CRISPR-Cas9: Tips for Optimizing sgRNA Activity by John Doench All CRISPR Posts  ...

20. PCR Applications for Copy Number Variation Assays

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    Blog Post

    ...amplified in the same reaction. Taqman’s increased sensitivity allows for a more confident determination of ...