We narrowed to 573 results for: Vit

1. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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   Blog Post

   ...Canton Nucleic Acids Forum (CNAF) 2015 conference invited me to attend and speak about Addgene’s experience...

2. How-to: 5 Steps to a Great Panel Discussion

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   Blog Post

   ...incorporate them into the program. I have to admit to inviting interesting friends and colleagues to events for...

3. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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   Blog Post

   ... Doench CRISPR-Cas9: Tips for Optimizing sgRNA Activity by John Doench All CRISPR Posts  ...

4. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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   Blog Post

   ...for trimethoprim, there would be no changes in sensitivity. Pooled knockdowns are also possible with Mobile-CRISPRi...

5. PCR Applications for Copy Number Variation Assays

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   Blog Post

   ...amplified in the same reaction. Taqman’s increased sensitivity allows for a more confident determination of ...

6. Polymerase Chain Reaction Overview and Applications

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   Blog Post

   ...a polymerase chain reaction (PCR). PCR is an in vitro method in which a small amount of DNA can be copied...

7. Production of Virus in Insect Versus Mammalian Cells

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   Blog Post

   ...insectspodoptera frugiperda (lepidoptera; noctuidae). In Vitro, 13(4), 213–217. https://doi.org/10.1007/bf02615077...

8. Tips for a 1st time AAV user (by a Rookie AAV user)

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   Blog Post

   ...recombinant adeno-associated viral vectors for in vitro and in vivo use. Current protocols in molecular ...

9. Plasmids 101: 5 factors to help you choose the right cloning method

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   Blog Post

   ...give Gateway a try. This method, developed by Invitrogen and based on lambda phage integration sequences...

10. Plasmids 101: Protein Expression

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    Blog Post

    ...cell-free expression systems, proteins are assembled in vitro using purified components of the transcription and...

11. AAV Vector Quality Control: Going the Extra Mile with NGS

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    Blog Post

    ...contamination is a good start, but to determine the sensitivity of our method we wanted to see how low we could...

12. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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    Blog Post

    ...TRiP- Functional Genomics Resources website has in vitro reagents and tools to help you knock down, knock...

13. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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    Blog Post

    ...useful for your next localization experiment and invite you to make any fluorescent protein constructs ...

14. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

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    Blog Post

    ...Jung, D., Yoon, S., & Kim, H. H. (2019). SpCas9 activity prediction by DeepSpCas9, a deep learning–based...

15. Twenty Years of Addgene Sharing: CRISPR

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    Blog Post

    ...of new tools, illustrated in Figure 2, and the longevity of tools seen in Figure 3, indicates that the ...

16. PhD Applications After COVID

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    Blog Post

    ... receiving applications, universities typically invite a select group of students to continue the application...

17. Addgene's Cancer Collection Pages Connect the Research Community

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    Blog Post

    ...to include several other important pathways. We invite feedback and contributions from all members of ...

18. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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    Blog Post

    ...Importantly, all of these assays are performed in vitro. Assays to detect FP dimerization in in vivo cellular...

19. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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    Blog Post

    ...groups. What apps do you use to improve your lab productivity? As this is a growing market, I am sure I missed...

20. Plasmids 101: Multicistronic Vectors

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    Blog Post

    ...RNA directs internal entry of ribosomes during in vitro translation. Jang et al. (J Virol. 1988 Aug;62(8...