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We narrowed to 835 results for: ang

Showing: 481 - 500 of 835 results
  1. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...tracrRNA pair or an sgRNA. It is often used interchangeably with the term sgRNA. Seed sequence: The 8-10...cheat sheet for that! Resources Further reading Wang, J. Y., Pausch, P., & Doudna, J. A. (2022). Structural...
  2. What's the Best Way to Elute and Store Your Plasmid DNA?

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    Blog Post
    ...contamination.  Extreme pH can denature, break, and even change the DNA sequence. High pH favors the breakage of...for the sugar backbone of the DNA helix or even changes the DNA sequence through a mechanism called depurination...
  3. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...sequences and then testing various combinations of changes. The basic functional version of the Sleeping Beauty...contain two SfiI restriction sites with different overhangs depending upon whether they are on the 3’ or 5...
  4. Summer SciComm Series: Master of SciComm

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    Blog Post
    ...past and chatting with individuals whose research ranged from wildlife conservation to abortion law to poetry...confluence of science and the world it hopes to change. Most researchers are aware of the importance of...
  5. RUBY-Red Siliques

    Type
    Blog Post
    ...site linkages and a terminator. Image from He, Y., Zhang, T., Sun, H. et al. Hortic Res. (2020).   I .... References and Resources References  He, Y., Zhang, T., Sun, H. et al. A reporter for noninvasively...
  6. New and Upcoming Viral Vectors - Spring 2019

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    Blog Post
    ...single-wavelength sensors with intensities that change in response to calcium binding. There are a few... through imaging. Since it’s illuminated by red/orange light, Archon1 can also be used in conjunction ...
  7. A Tour of Addgene's Most Popular Pooled Libraries

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    Blog Post
    ... Overexpression of Regulatory Factors. The Feng Zhang lab developed this library of all human transcription...ExpRession Element. This library consists of broad host range regulatory sequences ready to be assembled with ...
  8. 10 Great Guest Posts We're Thankful For

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    Blog Post
    ...and virology Minigenomes - A Safe Way to Study Dangerous Viruses Contributed by guest blogger Tessa Cressy...and retract over many microns in response to pH changes without the need for ATP or other chemical fuel...
  9. Viral Vectors 101: Transductions

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    Blog Post
    ...virus to deliver nucleic acids? Sounds sort of dangerous at first suggestion! The good news is that viruses... the right safety considerations, they aren't dangerous for controlled lab usage. Using viral transductions...
  10. Viral Vectors 101: Parts of the AAV Transfer Plasmid

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    Blog Post
    ...which have recognition sites in the ITRs. Regular Sanger sequencing typically fails to sequence through ...and high GC content, but some companies now offer Sanger sequencing conditions which can read through this...
  11. Using CRISPR/Cas9 to Edit Disease Out of the Genome

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    Blog Post
    ...Cas9 has been used to generate mutations in a wide range of organisms, but its potential to efficiently correct...the use of nanoparticles. [6].    References 1. Zhang B. 2021. “CRISPR/Cas gene therapy.” Journal of cellular...
  12. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...fusion protein binds to glutamate, conformational changes lead to an increase in fluorescence intensity from...properties such as kinetics, ∆F/F0, SNR, dynamic range, photostability, quantum yield, and expression. ...
Showing: 481 - 500 of 835 results