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  1. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    ...are ever recycled, so adopting a few meaningful changes in the lab could make a big difference in our global...Many scientists are already leading the cultural change toward responsibility in our lab’s environmental...

  2. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...tracrRNA pair or an sgRNA. It is often used interchangeably with the term sgRNA. Seed sequence: The 8-10...cheat sheet for that! Resources Further reading Wang, J. Y., Pausch, P., & Doudna, J. A. (2022). Structural...

  8. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...sequences and then testing various combinations of changes. The basic functional version of the Sleeping Beauty...contain two SfiI restriction sites with different overhangs depending upon whether they are on the 3’ or 5...

  9. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...and virology Minigenomes - A Safe Way to Study Dangerous Viruses Contributed by guest blogger Tessa Cressy...and retract over many microns in response to pH changes without the need for ATP or other chemical fuel...

  10. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...which have recognition sites in the ITRs. Regular Sanger sequencing typically fails to sequence through ...and high GC content, but some companies now offer Sanger sequencing conditions which can read through this...

  11. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    ...single-wavelength sensors with intensities that change in response to calcium binding. There are a few... through imaging. Since it’s illuminated by red/orange light, Archon1 can also be used in conjunction ...

  13. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...past and chatting with individuals whose research ranged from wildlife conservation to abortion law to poetry...confluence of science and the world it hopes to change. Most researchers are aware of the importance of...

  14. RUBY-Red Siliques

    Type
    Blog Post
    ...site linkages and a terminator. Image from He, Y., Zhang, T., Sun, H. et al. Hortic Res. (2020).   I ...  References and Resources References  He, Y., Zhang, T., Sun, H. et al. A reporter for noninvasively...

  15. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...virus to deliver nucleic acids? Sounds sort of dangerous at first suggestion! The good news is that viruses... the right safety considerations, they aren't dangerous for controlled lab usage. Using viral transductions...

  17. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ... The space was modern with clean bold colors of orange and white. The decor was very contemporary and ...hours. It was robust, capable of generating a wide range of insert sizes and also produced high yields of...

  19. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...ability to remove, reset and ligate mismatched overhangs, yielding scarless DNA assembly, and allowing ...mixture. The exonuclease removes mismatched 3’ overhangs, so that the region of homology doesn’t have to...

  20. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...TRICK reporter mRNA this displacement leads to a change in the fluorescent signal: untranslated RNAs appear...experiments, it’s important to verify that these changes do not impact translation rate, as the authors ...
Showing: 481 - 500 of 827 results