We narrowed to 835 results for: ang

...Sometimes DNA sequences need to be “cleaned up” or changed in some way before you can analyze them. These ...Data-optimized Assembly Design (DAD): This tool designs overhang sets for high complexity Golden Gate Reactions...

... suggest changes to our social media strategy. We ended up implementing some of these changes and seeing...

...tracrRNA pair or an sgRNA. It is often used interchangeably with the term sgRNA. Seed sequence: The 8-10...cheat sheet for that! Resources Further reading Wang, J. Y., Pausch, P., & Doudna, J. A. (2022). Structural...

...matter expert in the educational sphere, it can range from researching an area, to writing an introduction...lab classes. However, their opinions are often changed after seeing a kit for themselves. “You open up...

...growth is possible even if your job title doesn’t change. Master’s level training can prepare you to present... career with a master’s. While it's possible to change fields of research with a PhD, a master’s is less...

...conformational change in the GPCR, leading to the activation of the Gα subunit. The activated Gα exchanges bound...

...Introducing changes and/or short peptides sequences into these variable loops is one method for changing a capsid...

...contamination.  Extreme pH can denature, break, and even change the DNA sequence. High pH favors the breakage of...for the sugar backbone of the DNA helix or even changes the DNA sequence through a mechanism called depurination...

...sequences and then testing various combinations of changes. The basic functional version of the Sleeping Beauty...contain two SfiI restriction sites with different overhangs depending upon whether they are on the 3’ or 5...

...past and chatting with individuals whose research ranged from wildlife conservation to abortion law to poetry...confluence of science and the world it hopes to change. Most researchers are aware of the importance of...

...site linkages and a terminator. Image from He, Y., Zhang, T., Sun, H. et al. Hortic Res. (2020).   I .... References and Resources References  He, Y., Zhang, T., Sun, H. et al. A reporter for noninvasively...

...single-wavelength sensors with intensities that change in response to calcium binding. There are a few... through imaging. Since it’s illuminated by red/orange light, Archon1 can also be used in conjunction ...

...looking to improve their AAV yields. Given the wide range of downstream applications scientists rely on our...): 989-993. PubMed PMID: 18597326. 5. Shen, Chun Fang, and Amine Kamen. "Hyperosmotic pressure on HEK ...

... Overexpression of Regulatory Factors. The Feng Zhang lab developed this library of all human transcription...ExpRession Element. This library consists of broad host range regulatory sequences ready to be assembled with ...

...and virology Minigenomes - A Safe Way to Study Dangerous Viruses Contributed by guest blogger Tessa Cressy...and retract over many microns in response to pH changes without the need for ATP or other chemical fuel...

...virus to deliver nucleic acids? Sounds sort of dangerous at first suggestion! The good news is that viruses... the right safety considerations, they aren't dangerous for controlled lab usage. Using viral transductions...

...which have recognition sites in the ITRs. Regular Sanger sequencing typically fails to sequence through ...and high GC content, but some companies now offer Sanger sequencing conditions which can read through this...

...: to understand how a mouse changes emotions, to see how a monkey changes decision making, to cure a human...

...Cas9 has been used to generate mutations in a wide range of organisms, but its potential to efficiently correct...the use of nanoparticles. [6].    References 1. Zhang B. 2021. “CRISPR/Cas gene therapy.” Journal of cellular...

...fusion protein binds to glutamate, conformational changes lead to an increase in fluorescence intensity from...properties such as kinetics, ∆F/F0, SNR, dynamic range, photostability, quantum yield, and expression. ...