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We narrowed to 951 results for: cha

Showing: 481 - 500 of 951 results

  1. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    ...s Lab in 2009. From 2009 to today, Sample6 has changed focus and names, swapped leadership, and grown ...much learning, the focus of the application was changed, but Sample6 still leverages the core engineering...his co-founders raised $6 million. The biggest challenge during this time was learning to communicate effectively...better than current technology and get customers to change behavior. When you are ready to commit to the company...

  2. Antibodies 101: Conjugation

    Type
    Blog Post
    ...and ELISAs. Chromogenic reactions produce a color change, so they are typically used in microscopy-based...coupling can be achieved through several different mechanisms. Most take advantage of chemical groups intrinsically...that make up the disulfide bonds between antibody chains. 3) Endogenous carbohydrates added to antibodies...References Bendall SC, Nolan GP, Roederer M, Chattopadhyay PK (2012) A deep profiler’s guide to cytometry...

  3. The time and cost required to make a plasmid

    Type
    Blog Post
    ...restriction enzymes or vector purchases (Table 1). Restriction enzymes and vector purchases can raise the cost anywhere...one-off projects, or even save yourself hours by chatting quickly with the postdoc who’s really good at ...harder it is to throw in last minute experiments or changes. Fun fact: Addgene’s high-throughput approach means...

  4. Giving gRNAs a Facelift - Synthetic and Beyond

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    Blog Post
    ...kits or ordered as synthesis modifications when purchasing your gRNA. Pro tip: modifications are typically...expression systems are constantly “on”. There is no mechanism to turn cutting off or on at a specific time. ...and you can opt to do-it-yourself with a kit or purchase it commercially synthesized with a label. Not ..., E., Satyapertiwi, D., Ramel, MC., et al. Photoswitchable gRNAs for Spatiotemporally Controlled CRSIPR-Cas-based...

  5. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type
    Blog Post
    ...monkeys. Surprisingly, we found that this small change made a big difference. Replacing the mCherry with...does highlight the importance of a careful characterization of transgene expression. Which tag should ...RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method...Raper J, Hu X, Paré J, Bonaventura J, Richie CT, Michaelides M, Mueller SAL, Roseboom PH, Oler JA, Kalin NH...

  6. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    ...protein homodimerization is characterized by an affinity of 110 µM (Zacharias et al, 2002), meaning that... proteins reported in publications are only characterized under a limited number of conditions, often ...microscopy (University of Amsterdam). He develops, characterizes and uses genetically encoded fluorescent probes...

  7. Celebrating 15 Years of Scientific Sharing

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    Blog Post
    ... Joanna Brownstein (our outgoing Chair) and Dave Root (incoming Chair) and our entire supportive Board...year (12% in 2018) since 2005. We’re meeting the challenges of DNA distribution to ensure scientists everywhere...happening. The Addgene team will have some fun challenges this year and I’m excited about this, but definitely...

  8. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ... studying protein turnover is radioactive pulse-chase experiments. However, these experiments are problematic...synthesis: incorporation of puromycin into peptide chains." Proceedings of the National Academy of Sciences...PubMed Central PMCID: PMC4414919. 5. Yarmolinsky, Michael B., and L. Gabriel. "Inhibition by puromycin of...Single β-actin mRNA detection in neurons reveals a mechanism for regulating its translatability." Science 343.6169...

  9. Summer SciComm: Preprints

    Type
    Blog Post
    ...scientists communicate with each other: from formal mechanisms like publishing a research article, presenting...informal settings like lab meetings, Twitter, coffee chats with potential collaborators, blogging and more....read freely by anyone and are typically free of charge for authors to post. They can be assessed and commented...authors over time, providing a public record of changes, critiques or endorsements. In contrast, journal-based...

  10. Antibodies 101: Selecting the Right Antibody

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    Blog Post
    ... antibody cross-reacts to, then there is a good chance that the antibody could meet your needs.    Multiplex...will not only have peace of mind as you make your purchase but also someone to turn to for advice if you ...application.      Fig. 1: Antibody Selection Flow Chart    What does validated mean anyway? You now ...Validation!  Download our antibody selection flow chart.  References and Resources Additional Resources...

  11. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    ...CRISPR/Cas9 genome editing can result in unwanted changes at non-target sites. Means to decrease these so-called...between the non-target strand and a positively charged groove formed by the Cas9 HNH and RuvC nuclease...al reasoned that, if they decreased the positive charge in the HNH/RuvC groove, then they could weaken ...interactions between the groove and the negatively charged DNA and therefore destabilize basal strand separation...

  12. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type
    Blog Post
    ... with Cas9 guided by different promoters. The challenge in building genetic circuits is getting enough...shape of the response function or how the output changes as a function of input. Usually that function is...enough genetic parts for complexity. We have characterized almost 600 terminators. That gives us enough... we’ve really been making tons of parts and characterizing the software to help you put it together. The...

  13. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    ...experiments are either not accessible or too challenging for amateur scientists. Planarians, wormy creatures...cell types and for viewing how gene expression changes through time or after genetic manipulation. The...tissue sample, several washes, and temperature changes to visualize the expression of specific genes. ...genes of interest and was first discovered and characterized in an unrelated worm (C. elegans) [2]. Delivering...

  14. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...Indicate the amino acid change as well as any functional consequences of that change. For example, “Mutated... Answer “What does this plasmid do?” in <200 characters. A descriptive purpose focuses on the practical...Addgene Blog Find Articles Citing Addgene Plasmids Searchable and Sortable gRNAs for Your Next CRISPR Experiment...

  16. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...of the theme of my interview stories as the characteristic which the story demonstrates I possess.   Bullet...to present about your prior research. This is a chance to highlight your prior accomplishments and demonstrate...carefully. And even if you don’t have technical challenges, your interviewers might.  You’ll probably still...their hand to ask a question or type it into the chat. Either way, just let the interviewers know up front...

  17. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...Shyu YJ, Liu H, Deng X, Hu CD. Biotechniques. 2006 Chang-Deng Hu Green sfGFP Split super-folder GFP, our...protein-protein interactions in vivo. Blakeley BD, Chapman AM, McNaughton BR. Mol Biosyst. 2012 Brian McNaughton...Shyu YJ, Liu H, Deng X, Hu CD. Biotechniques. 2006 Chang-Deng Hu Venus Improved N-terminal fragment VN155...signal-to-noise ratio. Kodama Y, Hu CD. Biotechniques. 2010 Chang-Deng Hu mVenus pET-BiFC contains both fragments...

  18. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...tasks, both of which are factors in the technical challenges they can present. Many researchers find themselves... following transfection. In other words, when exchanging the medium after transfecting cells, replace ...electrostatic forces between these two negatively-charged membranes (Davis et al., 2002). Polybrene is one...306. PubMed PMID: 10634178. 8. Murnane, John P., Michael J. Yezzi, and Barbara R. Young. "Recombination ...

  19. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    ...the only right way, and Indecisives, who keep changing their minds and have trouble making decisions.... of your supervisor’s management style, bring challenges to his or her attention as soon as you can. It... action items. This will give your supervisor a chance to review what you have discussed and add suggestions...mutually beneficial solutions. If you package your challenges with your supervisor into learning opportunities...

  20. Viral Vectors 101: Producing Your rAAV

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    Blog Post
    ...titration by qPCR.   Pro tip! An optional media exchange can be performed 12–18 hours post-transfection...the density gradient material by dialysis/buffer exchange and further concentrate the virions if needed....Lock, M., McGorray, S., Auricchio, A., Ayuso, E., Beecham, E. J., Blouin-Tavel, V., Bosch, F., Bose, M., ...., Francis, J. D., … Snyder, R. O. (2010). Characterization of a recombinant adeno-associated virus type...
Showing: 481 - 500 of 951 results