Tips for CRISPR Gene Editing in Mice
Type
Blog Post
...determine the pros and cons of using many different types of gRNA design software.
When you are happy with...
We narrowed to 522 results for: pes;
-
-
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog Post... in vivo studies. Let’s take a look at the many types of plasmid addiction systems and their applications... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog Post...screen presented in Morgens et al. contains three types of guides: 1) targeting guides (blue), which target... -
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog Post...replication machinery of Kaposi’s sarcoma-associated herpesvirus. References 1. Snapp, Erik. "Design and... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog Post...of c-fos amongst animals that receive different types of stimulation may help to refine and optimize your... -
Plasmids 101: E. coli Strains for Protein Expression
TypeBlog Post...be discussed later). In addition to the basic genotypes outlined below, certain specialized strains are... -
Antibodies 101: Single Chain Fragment Variables (scFvs)
TypeBlog Post...ScFvs can also be fused to cellular toxins, radioisotopes, cytokines, and enzymes for cancer, autoimmune... -
Five Popular Model Organisms, Part 2
TypeBlog Post...Pisula, W. (2020). The Norway rat, from an obnoxious pest to a laboratory pet. ELife, 9. https://doi.org/10.7554... -
Five Popular Model Organisms
TypeBlog Post... diseases. Diseases in mice show many similar phenotypes to human diseases and have successfully helped... -
Plasmids 101: Biotinylation
TypeBlog Post...using a biotin labelled dUTP. There are multiple types of biotin labels that can be added to an oligonucleotide... -
Adeno-associated Viruses (AAVs) for Genome Editing
TypeBlog Post...of choice for gene delivery to a variety of cell types, now you know that AAVs can provide a powerful boost... -
Deep Mutational Scanning with One Pot Saturation Mutagenesis
TypeBlog Post...Disadvantages of this method include favoring of certain types of mutations, difficulty controlling the rate of... -
CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9
TypeBlog Post....2969 Waltz E (2016) Gene-edited CRISPR mushroom escapes US regulation. Nature 532:293–293 . https://doi.org... -
Fluorescent Proteins 101: When GFP lets you down
TypeBlog Post...fluorescent protein in almost all organisms and cell types, (ii) no need to add a co-factor, (iii) easy visualization... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog Post...stranded breaks (DSBs) into genomic DNA in many cell types and often results in biallelic modifications. Most... -
Treating Muscular Dystrophy with CRISPR Gene Editing
TypeBlog Post...methods, they each observed the same positive phenotypes in cardiac and skeletal muscle. Even when the... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog Post... 25590106. Paula Stephan’s Book - How Economics Shapes Science The Future of Research Website Additional... -
Plasmids 101: Common Lab E. coli Strains
TypeBlog Post...reference for some of the popular strains, their genotypes, and their primary use in the lab. These strains... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog Post... targeted delivery into hard-to-manipulate cell types. They have also fused LSD1 to x-Cas9(3.7). x-Cas9... -
A Control for All Seasons
TypeBlog Post...will have a better understanding of the different types of controls and be able to use this information ...