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We narrowed to 934 results for: por

Showing: 481 - 500 of 934 results
  1. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

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    Blog Post
    ...degree is a means to gain employment, so it’s important to make sure a masters helps you get a job you...from the bench, look for “technical or product support specialist,” “field application scientist,” or ... career ladder is larger and there are more opportunities for promotion. It’s not uncommon for bachelor...
  2. Addgene at the ASM Microbe 2016 Conference

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    Blog Post
    ...worldwide and the presentation made it clear how important it is for us to improve our resources for this...Needless to say we all got very good at applying temporary tattoos on the spot with our trusty spray bottles...microbiology community #ASMMicrobe2016 was a great opportunity for Addgene to let microbiologists know about...
  3. Sleeping Beauty Awakens for Genome Engineering

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    Blog Post
    ...therapy - avoiding the use of viruses is also important for social and regulatory reasons. 1.) The transposon...puromycin, hygromycin) and fluorescent protein reporters (GFP, BFP, RFP, no FP). The vectors each contain...region of the RPL41 gene, to drive the selection/reporter parts of each construct. This new tool kit allows...
  4. Management for Scientists: Managing vs. Leading

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    Blog Post
    ...” instead of levels because it emphasizes the importance of these very different roles. At any stage in...doing, managing or leading. All three roles are important to accomplishing any goal or finishing any project... or highlighting criticisms. To be given the opportunity to take on the role of leader, one needs to take...
  5. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

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    Blog Post
    ...primary antibody that is directly conjugated to your reporter circumvents the issue. However, directly conjugated...which uses just a primary antibody that has your reporter (in this case a fluorophore) directly attached... as effective for other techniques, but it is important to be aware that the issue is not unique to IHC...
  6. Multifunctional Neural Fibers: Modulation from Gut to Brain

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    Blog Post
    ...included in them for downstream control – this is important later! Next, additional components are added -...times more flexible than the brain fibers and importantly distortion of the fibers didn’t affect their ...temperature proximal to the implanted fibers. As important as its capabilities are the practical considerations...
  7. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

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    Blog Post
    ...new tools and protocols that they can easily incorporate into their own labs. This week we are pleased...following sucrose treatment. Moreover, several reports have shown that cells treated with sucrose or other...test this approach with different capsids. Most importantly, because our AAV yields are improved we will ...
  8. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

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    Blog Post
    ...probes, such as antigen-binding fragments (Fabs) (Porter 1959) and single-chain variable fragments (scFvs...of antibody-combining sites within the variable portions of heavy and light chains. Proceedings of the ... PMID:4560694. PubMed Central PMCID: PMC427010. Porter, R. (1959). The hydrolysis of rabbit y-globulin...
  9. Science Careers: Unruly Interests Feed Many Paths

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    Blog Post
    ...stylistic consistency. If you’ve ever doubted the importance of copy editors, the New Yorker’s “Comma Queen...review were discussed. Science communication opportunities If you are interested in the science communications...summer program that gives graduate students an opportunity to work in radio, print journalism, and other...
  10. In Vivo Biotinylation of Bacterial Fusion Proteins

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    Blog Post
    ...purified protein involves immobilization on a solid support. This allows washing away of unbound material to...binding kinetics of the target ligand. Another important consideration is whether the affinity of an experimental...acids. Unfortunately, the location of biotin incorporation and to a lesser extent, the number of added ...
  11. Identifying Sequence Elements with SnapGene's Feature Database

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    Blog Post
    ...with every possible option, we place the most important controls front and center, and make specialized... are in the pipeline. First, now that SnapGene supports “Collections”, which are shareable databases of... missing from our database, or if you see an opportunity to harness common feature detection in a new ...
  12. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

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    Blog Post
    ...(Addgene #104102 - #104105) using in utero electroporation or with AAVs (prepared with Addgene #104110...Figure 3) that can be introduced using in utero electroporation. Then at your preferred time points, sacrifice...with an index-optimized clearing agent." Cell reports14.11 (2016): 2718-2732. PubMed PMID: 26972009. Ke...
  13. Plasmids 101: TOPO Cloning

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    Blog Post
    ...salt causes arcing during electroporation which would cause the electroporation to fail). When incubating...limit (lower transformation efficiencies have been reported with longer incubation); however, you may need...
  14. Don’t Wait To Launch Your Health Science Startup

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    Blog Post
    ...sort of talent agency for scientists, where we support health science graduate students with innovative...fund that would apply a percentage of returns to support student projects, but it quickly became apparent...The composition of the management team is more important than the product itself -- investors would rather...
  15. Hot Plasmids: FRET-Based Biosensors

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    Blog Post
    ...said. “We know CREB is really important, but where is it really important and when? That is not known.”...calcium-binding sites as possible for added sensitivity. As reported in Nature Methods, the FRET changes of these Twitch...
  16. The MTA at Addgene

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    Blog Post
    ...facilitate a fast and agile MTA process. This is important to keep up with the number of requests and deposits...for both research and commercial purposes. It’s important to note that it’s the depositing institute that...signatures from two different institutes. Our online portal automates a lot of the process, from notifications...
  17. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

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    Blog Post
    ...-hyg will be used to create gene deletions of important unknown regulatory regions in Pseudomonas and ... into a bacterial strain. The sensor will be transported inside a small 3D printed robot that will “swim...the user.   At Addgene we're happy to be able to support diverse groups of scientists working in a wide ...
  18. Scientists Map the SARS-CoV-2-Human Interaction Network

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    Blog Post
    ...physiologically-relevant systems, to catch all the important interactions. It’s also important to determine if the human interactors...includes a GFP construct as a control. For easier portability, all viral genes are flanked by EcoRI and BamHI...
Showing: 481 - 500 of 934 results