We narrowed to 630 results for: proc

1. 5 Tips for Troubleshooting Viral Transductions

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   Blog Post

   ...volume. Always remove packaging cell debris before proceeding to ultracentrifugation to avoid contaminating...

2. Cpf1 Update: Comparison to Cas9 and NgAgo

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   ...overhangs. Exploring the efficiency of these two processes will help us better understand where Cpf1 fits...

3. Plasmids 101: FLEx Vectors

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   ...SSR of choice is introduced, recombination can proceed either by first utilizing the loxP sites or the...

4. Tips for Titering Your Lentiviral Preps

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   ...subcloning, expansion, and confirmation. This procedure can take several months but should be considered...

5. The Materials Science of Optogenetics Experiments

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   ...animal’s skull using dental cement (a similar procedure as implanting a guide cannula). A fiber optic ...

6. How-to: 5 Steps to a Great Panel Discussion

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   Blog Post

   ... Your audience may have a lot of real or self-proclaimed experts on the topic at hand. You can use this...

7. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

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   Blog Post

   ... thing into a marketable product. During this process, we had an idea that survived the literature review...

8. Hot Plasmids and Viral Preps - May 2021

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   ...could be used to study the role of aaRSs in these processes. To create the antibody toolbox, they screened...

9. 3D Printing Meets CRISPR Cas9

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   ...protein. At some point during this interrogation process, they will almost invariably say something like...

10. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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    ...container before the progeny begin eclosion, the process of hatching from their pupal case (~9 days after...

11. Fluorescent Proteins 101: Fluorescent Protein Timers

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    ...allowing them to optimize the compound production process. How have you used FP timers? Are you planning ...

12. Hot Plasmids: Summer 2025

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    ...vitro and uses them in our own AAV production processes. Check out our step-by-step protocols for AAV ...

13. Hot Plasmids: Winter 2025

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    ... inserted. B) Protein expression and labeling procedure. Adapted from Smith et al. 2024 under a CC-BY ...

14. Pooled Library Amplifications

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    ... reduce recombination during the amplification process. In liquid culture, all of the bacteria can grow...

15. Antibodies 101: Flow Compensation

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    ...In flow cytometry, compensation is the process of correcting spillover from one fluorescent channel to...

16. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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    ...types makes them useful models for developmental processes and promising therapeutic tools. The two unique...

17. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

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    ...indeed possible, with correct, in-frame assembly proceeding in 99% of 12-fragment assemblies and over 90%...

18. 10 Basic tips for mammalian cell culture

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    ...bisphenol A-induced perturbation in biological processes in ER-negative HEK293 cells." PLoS One, 2014. ...

19. Addgene's Cancer Collection Pages Connect the Research Community

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    ...from rogue cells when something does go awry. The process of programmed cell death, or apoptosis, is one ...

20. Experimenting with New Careers while in Grad School

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    ... comfortable terms helped guide me through the process. As I mentioned, my graduate office staff was amazingly...