...in adult animal models results in rapid symptom onset, suggesting that MECP2 is also essential after development... gene was disrupted and silenced by a Lox-Stop cassette, resulting in the neurological deficits observed...Restoring the MECP2 gene by excision of the Stop cassette using tamoxifen-inducible Cre not only halted ...

... an organism uses only a subset of codons, it only needs to produce a subset of charged tRNAs and therefore...increase their abundance. For example, the commercial Rosetta E. coli strains express a variety of tRNAs that...

...courtesy of IPI. IPI anti-integrin toolset IPI’s anti-integrin toolset comprises 26 recombinant antibodies... that bound an amino acid region displayed by a subset of the RGD-binding integrins containing the αV ...

...translation-blocking STOP cassette immediately following the promoter. The STOP cassette includes a mutant YFP...eliminating two color possibilities. The remaining cassette can invert as long as Cre remains expressed; once...

...responses to viral infections have resulted in the onset of cancer. Similar patterns of cancer development...inflammatory bowel disease is associated with the onset of colorectal cancer. Strategic immune evasion mechanisms...cytotoxic effector T cells, and targeting specific DC subsets in vivo using specific antigens to activate them...

...Experiments by Jennifer Tsang Chromoproteins are a subset of the fluorescent protein family and they generate...molecular biology tools out there! Here, we highlight a subset of these tools for plasmid mapping, DNA/protein...

... in the dataset; 2) users not part of a scientific Twitter list were excluded from the dataset. 3. 60%...

... (a specific mutation in a known gene = disease onset) may be best served by this type of targeted design...hits’ - genes that affect disease progression or onset. These hits can reveal individual genes or sometimes...

...recycle our discards according to the Cambridge, Massachusetts guidelines and wear an extra sweater so we don... for smaller and smaller gel apparatus until I settled on using glass slides. With just the right amount...

... only lead to a better understanding of disease onset, but also better simulate a disease in a model system... custom TF programs to acutely initiate disease onset, providing both a tool to test prophylactics on ...

...up positive traits (i.e. the plasmid contains a cassette that will make cells resistant to a toxin), whereas... a negative trait (i.e. the plasmid contains a cassette that will make cells sensitive to a toxin). In...

...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids... Weiss’s group used these reporters to define a subset of active miRNA’s unique to HEK293FT and HeLa cell...

...found to have a point mutation in the puromycin cassette that makes it less efficient in some cell lines... It contains a point mutation in the puromycin cassette that makes it less efficient in some cell lines...

...career, I’d have realized it was a great fit for my skillset and interests. As such, I want to be able to share...re building software will help you be a valuable asset to the scientists as well as the developers. Lastly...

...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...proximity to the GFP-tagged protein (albeit slightly offset due to the nanobody-GFP interaction). The modularity...

...RNAs (gRNAs) are commonly packaged in 400-500 bp cassettes containing the RNA pol III promoter, gRNA and ...and pol III terminator. These relatively large cassettes (considering the gRNA itself is ~100 bases) limit...

...biochemistry department at the University of Massachusetts Medical School. We are working with postdocs...to hold. There are institutions in Boston - Massachusetts General Hospital, Mass Eye and Ear Infirmary...

...then subtracted from Ramanathan et al.’s RaPID datasets. Applications of RaPID RaPID sounds like a nifty...characterized by increased iron serum levels and early-onset cataracts, is characterized by point mutations in...

...carries CRE recombinase and a puromycin resistance cassette. No time to produce virus? Get the ready-to-use...Zhang lab expressing S. pyogenes Cas9 and a gRNA cassette. It can be used to produce a ~10-fold higher titer...

...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ... hybrid gRNA library deletes human alternative cassette exons and disrupts genes with alternative exons...