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...the performance of different nucleases. Nicola Patron’s lab compared the efficiency and specificity of...plasmids Anti-CRISPR proteins for optogenetic control of CRISPR-Cas9 Anti-CRISPR proteins that inhibit...has adapted anti-CRISPR proteins for optogenetic control of CRISPR-Cas9. By combining AcrIIA4, a Streptococcus...

...They kicked off their day with breakfast and an introduction to Addgene with our Executive Director, Joanne...in-depth look at some of the teams at Addgene. An introduction to plasmids with Director of Biology Lianna ...our UK team member Benoit Giquel. Bluegene’s globetrotting impressed our guests, as did how much work is...

...loops, SynORI uses synthetic RNA regulators to control replication. Additionally, we refactored the natural...regulatory RNAs designed computationally. Copy number control Beyond compatibility, SynORI makes copy number ...factors and riboswitches, can be used to dynamically control copy number. Eliminating multimers Plasmids in ...

...for use as a negative control, and empty backbones such as pBABE-puro for retroviral expression and pLVTHM...psPAX2. Actually, these two plasmids from Didier Trono's lab are our two most popular plasmids of the past...

... they do so by activating signaling pathways controlling properties like growth, survival, and differentiation...inactivated the pathway. “The idea is that if you introduce them into cells one at a time, you could identify... for pathways that drive resistance to drugs in vitro, and they’ve shown that it works. But, he says that...

...growth based on data voluntarily made available by Medtronic via The Yale University Open Data Access (YODA...got a nine percent bump in citations for their trouble, even when all kinds of other factors, including...universities. We particularly recommend Eric Lander's intro to biology course "The Secret of Life." 10)     ...

...for packaging: AAV1, AAV2, AAV5, AAV8, or AAV Retrograde (AAVrg) capsids.  Please be aware that this ordering...Packaged on Request prep and run the same quality control assays we run with all of our in-stock viral preps...plasmids packaged in AAV1, AAV5, AAV8, and AAV Retrograde to be 1x1013 and titers for AAV2 to be 7x1012...

...McClean’s lab generated an optogenetic toolkit that controls repression of gene expression in Saccharomyces...transcriptional repressor Mxi1, and an optogenetic tool that controls nuclear localization (LINuS). Blue light renders...mRNA decreased after 20 minutes in xylose (which controls expression of dCas9). The lab demonstrated the...

... system is toxic to proliferating cells. A Cre-estrogen receptor ligand binding domain-fusion can prevent... toxicity in proliferating Drosophila cells by estrogen-dependent activity regulation. Heidmann D, Lehner... BAC/PAC recombineering techniques. Semprini S, Troup TJ, Kotelevtseva N, King K, Davis JR, Mullins LJ...

... me like the postdocs are taking some steps to control their own future in science (see my previous blog...vehemently about the need for change.  Dr. Bourne strongly believes this is an amazing time of promise for... Novartis industry postdoctoral program.  His strongest comments were about the astounding lack of reproducibility...

...and/or the use of chelating agents that can be introduced in the purification protocol to remove endonucleases... your preparation for a long time. Tris buffer controls the pH, while the EDTA chelates any divalent cations...of using a Tris buffer is that it allows better control of the pH preparation stabilizing the plasmid, ...

...the workforce, but my main concerns were building strong connections with coworkers and doing something ...interested in knowing you as a person, builds a strong sense of trust between coworkers. Since I trusted...about making connections with coworkers seem, in retrospect, nonsensical. I have found the people at Addgene...

... sections of a paper, the abstract, figures, introduction, etc. so as to thoroughly explain the do’s and...reader is not in their head. The scientist has to introduce the reader to the manuscript and engage them in...to make the final product with an interesting introduction and concise discussion. Many thanks to our ...

...sequence the plasmid to confirm no mutations were introduced during PCR or any subsequent steps. Using high...DNA polymerases, I have never had any mutations introduced into my inserts. However, with any PCR-based ...PCR-based cloning technique there is a chance of introducing an unwanted mutation. Tips for high efficiency cloning...

...Getting into the office for the first time, being introduced to all the members around the office one by one...and undigested samples of the clones on an electrophoresis gel in order to differentiate which clone had...Once we had gotten our results back from the electrophoresis, my manager and I were unsure if there were...

...recognition come from the successes of the team. A strong sense of engagement in the work Behind-the-scenes...    This is the first in a 5 part series on Introduction to Managing People for Scientists. Subscribe...follow the entire series as it is published. Good introductory reference books on managing people: Skills ...

...function of Your Gene Of Interest (YGOI) in a controlled environment under various conditions. The first...expression system and go for a lentiviral or a retroviral backbone. This is considered to be a more robust...great for overexpressing YGOI in an isolated, controlled environment. In most cases bacterial expression...

... store a record of invading phages but to also destroy the phage upon re-exposure (2, 3). More specifically...complex binds to the foreign DNA, a cut is made to destroy the invader. Wikipedia, accessed 25 November...ultimate victor will be the species who has the strongest will to survive. Genome engineering and future...

...interests and areas of expertise. Perhaps the strongest aspect of science communication, then, is its ...address this obstacle in its many forms. A short stroll from my office to the tearoom would often illustrate...Cognitive LoadSummer SciComm Series: Modes of CommunicationIntro to Our Summer SciComm Series!...

...cases, pay special attention to designing your controls in validation, as you may need to test different...great deal of frustration when designing and troubleshooting immunoimaging assays. It can also elucidate...Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction to Antibodies Resources on Addgene.org ...