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Showing: 481 - 500 of 529 results

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Hot Plasmids - October 2020

Type

Blog Post

...greater number of red cells as measured by flow cytometry. Cells grown in high IL-3 showed mostly blue fluorescence...
Corralling and Cornering the Goldilocks Antibody

Type

Blog Post

...compatible with the desired applications (such as immunohistochemistry (IHC) or western blot), and cost-effective...
Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

Type

Blog Post

... a patient’s blood), scientists can use mass spectrometry to determine the amino acid sequences of the...
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

Type

Blog Post

... Confirming the gene/insert by aligning to NCBI entry or using BLAST. Confirming tags and fusion proteins...
Illuminating Epigenetics with A FRET Based Biosensor

Type

Blog Post

...like the potential devastation of the palm oil industry through epigenetic effects on the Cover of Nature...
Cloning Mammalian Cells with the Agarose Method

Type

Blog Post

...line, and its transfection efficiency. We often trypsinize the transfected cells on the day after transfection...
Fluorescent Proteins 101: Fluorescent Protein Timers

Type

Blog Post

...fiber bundle formation." Journal of Biological Chemistry 276.32 (2001): 29621-29624. PubMed PMID: 11408473...
Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine

Type

Blog Post

...Jaenisch, R. and R. Young, Stem cells, the molecular circuitry of pluripotency and nuclear reprogramming. Cell...
PhD Applications After COVID

Type

Blog Post

...application cycle, two schools on opposite sides of the country had scheduled visitation days during my midterm...
Polymerase Chain Reaction Overview and Applications

Type

Blog Post

...Kary B. Mullis who later shared a Nobel Prize in Chemistry for his work. Since then PCR has become a standard...
Hot Plasmids and Viral Preps - May 2021

Type

Blog Post

...2020. Preger et al., Journal of Biological Chemistry 2020. https://doi.org/10.1074/jbc.RA120.01289 ...
Chromoproteins: Colorful Proteins For Molecular Biology Experiments

Type

Blog Post

...AnemoneCnidopus Japonicus. Journal of Biological Chemistry 281:37813–37819 . https://doi.org/10.1074/jbc....
Anatomy of a Plasmid Page at Addgene

Type

Blog Post

..., so some of our older plasmids may not have an entry in this field. If you’ve deposited a plasmid without...
Plasmids 101: Secondary Nanobody Toolbox

Type

Blog Post

... single-domain antibodies.” Annual review of biochemistry 82 (2013): 775-97. PubMed PMID: 23495938. Additional...
Tips for arabidopsis transformation

Type

Blog Post

...Analysis of Plants’, Bioscience, Biotechnology, and Biochemistry, 71(8), pp. 2095–2100. doi: 10.1271/bbb.70216...
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

Type

Blog Post

...those of their endogenous counterparts (by immunocytochemistry, for example), as this can also be perturbed...
Capturing a Moment with Marc Zimmer’s “The State of Science”

Type

Blog Post

...s a very big question. Zimmer, a Professor of Chemistry at Connecticut College who has been teaching and...
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

Type

Blog Post

...proteins,” ChIP-seq from Table 1. Because of DNA’s 3D geometry and the chemical properties of modified histones...
Tips from the Repository Trenches: Using Barcodes to Track Samples

Type

Blog Post

...lab team takes over and prepares the sample for entry into Addgene’s repository. For long-term storage...
Your Lentiviral Plasmid FAQs Answered

Type

Blog Post

...such reagents as outlined by your institution or country. Have any other questions? Feel free to post them...

Showing: 481 - 500 of 529 results

Results per page:

Hot Plasmids - October 2020

Corralling and Cornering the Goldilocks Antibody

Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

Illuminating Epigenetics with A FRET Based Biosensor

Cloning Mammalian Cells with the Agarose Method

Fluorescent Proteins 101: Fluorescent Protein Timers

Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine

PhD Applications After COVID

Polymerase Chain Reaction Overview and Applications

Hot Plasmids and Viral Preps - May 2021

Chromoproteins: Colorful Proteins For Molecular Biology Experiments

Anatomy of a Plasmid Page at Addgene

Plasmids 101: Secondary Nanobody Toolbox

Tips for arabidopsis transformation

Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

Capturing a Moment with Marc Zimmer’s “The State of Science”

CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

Tips from the Repository Trenches: Using Barcodes to Track Samples

Your Lentiviral Plasmid FAQs Answered

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