Plasmids 101: Modular Cloning
Type
Blog Post
...functions. Most individual parts have characterized activities with predictable behaviors. While a gene is the...
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Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog Post...but in contrast to lentiviral vectors, their infectivity is limited to dividing cells. Find tips on using... -
Adenoviral Vector Production and Troubleshooting
TypeBlog Post...Trapnell. "Evaluation of the concentration and bioactivity of adenovirus vectors for gene therapy." Journal... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog Post...subsequently destroyed by Argonaute-catalyzed nuclease activity or their translation disrupted through physical... -
Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux
TypeBlog Post...25653845. Pubmed Central PMCID: PMC4304227. 4. Revitalizing biomedical research: recommendations from the... -
5 Tips for Troubleshooting Viral Transductions
TypeBlog Post...given viral stock will give you a sense of the infectivity of your viral stock, save you time, and allow... -
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015
TypeBlog Post...Canton Nucleic Acids Forum (CNAF) 2015 conference invited me to attend and speak about Addgene’s experience... -
How-to: 5 Steps to a Great Panel Discussion
TypeBlog Post...incorporate them into the program. I have to admit to inviting interesting friends and colleagues to events for... -
Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria
TypeBlog Post...for trimethoprim, there would be no changes in sensitivity. Pooled knockdowns are also possible with Mobile-CRISPRi... -
AAV Vector Quality Control: Going the Extra Mile with NGS
TypeBlog Post...contamination is a good start, but to determine the sensitivity of our method we wanted to see how low we could... -
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies
TypeBlog Post...TRiP- Functional Genomics Resources website has in vitro reagents and tools to help you knock down, knock... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog Post...useful for your next localization experiment and invite you to make any fluorescent protein constructs ... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog Post...insectspodoptera frugiperda (lepidoptera; noctuidae). In Vitro, 13(4), 213–217. https://doi.org/10.1007/bf02615077... -
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
TypeBlog Post...Jung, D., Yoon, S., & Kim, H. H. (2019). SpCas9 activity prediction by DeepSpCas9, a deep learning–based... -
Twenty Years of Addgene Sharing: CRISPR
TypeBlog Post...of new tools, illustrated in Figure 2, and the longevity of tools seen in Figure 3, indicates that the ... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog Post... Doench CRISPR-Cas9: Tips for Optimizing sgRNA Activity by John Doench All CRISPR Posts ... -
PhD Applications After COVID
TypeBlog Post... receiving applications, universities typically invite a select group of students to continue the application... -
PCR Applications for Copy Number Variation Assays
TypeBlog Post...amplified in the same reaction. Taqman’s increased sensitivity allows for a more confident determination of ... -
Polymerase Chain Reaction Overview and Applications
TypeBlog Post...a polymerase chain reaction (PCR). PCR is an in vitro method in which a small amount of DNA can be copied... -
Tips for a 1st time AAV user (by a Rookie AAV user)
TypeBlog Post...recombinant adeno-associated viral vectors for in vitro and in vivo use. Current protocols in molecular ...