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We narrowed to 1,010 results for: RING;

Showing: 251 - 260 of 1010 results

  1. Technical Design of a Western Blot

    Type
    Blog Post
    ... assays are not with denaturing agents like DTT or β-mercaptoethanol.  Denaturing proteins To denature...blot, your loading buffer will not contain any denaturing agents. However, you’ll still have to lyse your... post, we are assuming that you are running a denaturing blot.  There are a number of lysing methods and... aliquot your sample(s) before adding in your denaturing and/or loading buffers, as most Bradford assays... proteins in different patterns, due to their differing chemistries. You therefore cannot compare Bis ...and reprobed multiple times. It doesn’t degrade during long-term storage and has both high binding capacity...well for proteins of most sizes and are worth considering if your proteins of interest vary wildly in size...

  2. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...research purposes, like creating stable cell lines, delivering CRISPR/Cas9 to cells for genome editing, and ... host’s genome isn’t uncommon. Many naturally occurring viruses occasionally integrate their genomes into...the host genome via the viral proteins packaged during production (read our blog post on the retroviral...time, IDLVs are ideal. As the episomes are lost during cell division, the expression of the delivered ...production protocol.  One thing to keep in mind is that titering these IDLVs may require a slightly different protocol...regular lentiviral vectors. This is because most titering methods rely on isolating genomic DNA from transduced...DNA along with a reference cellular gene. When titering IDLVs, there is no integrated piece to measure...

  3. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...be taken seriously so we made it official by registering BosLab inc. as a nonprofit organization in the...are skilled with audiovisual media, software engineering, business consulting, mathematics, and more. ... to perform proof-of-concept experiments for discovering novel therapeutics. Dave is a chemical biologist...and in his free time, he is pursuing a plant engineering project at BosLab. Once he optimizes plant cell...knowledge with the community by teaching a DIY plant engineering workshop. Community projects at BosLab Not all...and Mrina Nikrad have started the Open Yeast Engineering Project (OYEP). Through this project, community...do similar things. The focus is to make yeast engineering accessible to everyone but these projects could...

  4. Finding Your Perfect Job After University

    Type
    Blog Post
    ...problem solving. I believe that is something lacking during degree courses; they never really prepare you for...failure and problem solving scientists experience during research. Working abroad in rural Africa Once this...another area which I was really passionate about during my degree. I loved parasitology. However, when ... held me back; I do not have a second language. During the recession there were many qualified scientists...Genetics to give me an edge over other graduates during the recession. As each advertised job received ...the training and experience relating to good manufacturing practice (GMP) and good lab practice (GLP) (...recognised the logo, but didn’t know much else. During the interviews, I asked if I could contribute more...

  5. Addgene: The First Twenty Years

    Type
    Blog Post
    ...open science nonprofit that would facilitate the sharing of plasmids between scientists. The service would...resource. I really appreciate the work you do to make sharing reagents and protocols so easy!” — Nina Jain, Harvard...seven before we had 1,000 depositing scientists. Ensuring that people knew how to access Addgene, and that...2012, our repository was rapidly able to support sharing of CRISPR constructs and information across the...lentiviral and AAV vectors, as well as developing and sharing supportive educational resources. By 2017, we had...NGS run.   To twenty years and beyond! Which brings us to 2024, Addgene’s 20th anniversary and the ...been made because scientists believe in openly sharing their plasmids. It’s been a privilege to find and...

  6. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    ...head when you hear the phrase “action potential?” Firing neurons are likely the first thing that comes to...reprogrammed regenerating tissues without using genome engineering. Planarian worms cut into three segments “remember...communication is briefly inhibited in the entire worm during regeneration, the worm creates two heads. Subsequent...resources from the bioelectricity field In addition to sharing tools to study bioelectricity, bioelectric researchers...defects, regeneration, medicine, and synthetic bioengineering. References Chernet, Brook T., et al. "Use...growth and form." Annual review of biomedical engineering 19 (2017): 353-387. PubMed PMID: 28633567. Mathews...bioelectricity for regenerative and synthetic bioengineering." Current opinion in biotechnology 52 (2018...

  7. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...numbers of women participating in science and engineering. “Fix the Institutions” promotes gender equality...billions—but when drugs fail, they cause human suffering and death. Doing research right has the potential...between male and female cells. The result of not considering the sex of the stem cells was that their male...sex, and it’s important to analyze these factors during an experiment. In the case of stem cells, these...tissues—when that information is reliable—is important to ensuring excellence in basic research.  In addition to ... the sex of the most commonly used cell lines appearing in American Journal of Physiology-Cell Physiology...clue or potential place to start research is by considering sex disparities in disease severity. Such disparities...

  8. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...wash buffer such as PBS or TBST. Stringent stripping The stringent stripping solution uses heat and detergent...your sample? Or what if something weird happened during your western and your results look… funky? Do you...along with the antibodies, especially with more stringent stripping methods. If you are planning to detect...that section of the blot unusable for reprobing. Preparing your western blot Run your gel and membrane transfer...primary and secondary antibodies from your membrane, clearing the way for the second blot. There are multiple...with a mild stripping solution and move onto a stringent solution if the mild is not effective. You may...perhaps using longer incubation times, or try the stringent solution if you started with the mild one. Reprobe...

  9. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...of choice is AAV, with nearly every experiment requiring AAV. Before joining my lab, I had never worked... a heated stir plate to dissolve and requires monitoring so that the solution doesn’t get too hot because.... Rather than investing all of this effort in preparing reagents, I choose to only harvest the cell pellet...layered and then the viral suspension generated during AAV production is overlaid. Following ultracentrifugation...first purification. This helped me get a feel for layering the iodixanol solutions and let me double-check...make one or two small aliquots to use for qPCR titering. This helps avoid multiple freeze/thaws of your...of virus. AAV titration by qPCR Overview: AAV titering by qPCR quantifies the number of genome-containing...

  10. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ... steps, until its connection with DNA is broken during cross-link reversal (not shown in diagram). Cross-links...sequence targeted in the experiment. Figure 2: Comparing CAPTURE and CAPTURE 2.0.     Original CAPTURE...) one or more gRNAs Two separate lentiviruses delivering 1) a BioTAP-tagged dCas9 and an eGFP tag, and...detecting chromatin interactions opens the door for answering more questions about chromatin conformations. ...‘social networks’ of 157 super enhancers.  3. Measuring temporal changes in chromatin interactions A third...in chromatin conformation, like those that occur during development and cellular differentiation. Using...the Xu Lab tracked enhancer-promoter interactions during erythroid differentiation. CAPTURE 2.0 captures...
Showing: 251 - 260 of 1010 results