We narrowed to 911 results for: TRO

...spatiotemporal control over its expression. Constitutive or inducible Cre recombinases can be introduced by AAV...SPH mice CRISPRa has largely been limited to in vitro settings due to the large size of Cas9 activators...in vivo, they should be screened for activity in vitro and then packaged into AAV for in vivo delivery....developed neuron-optimized lentiviral vectors for in vitro and in vivo CRISPRa (Savell et al., 2019). This ...immediate-early gene Bdnf, which encodes brain-derived neurotrophic factor (BDNF). Bdnf is expressed early in response...of other CNS cell types, including microglia, astrocytes, and oligodendrocytes, provided that reporter...preferable for individual gene activation in select in vitro and in vivo applications, the tools described above...

...the vector to survive while the auxotrophic cells die. Some auxotrophic selection markers can be used across...autonomously replicating sequence (ARS), a partial centromere sequence (CEN), both, or neither, depending on...autonomously replicating sequence (ARS) a partial centromere sequence (CEN) both or neither ...depending ...choose to ensure it works in your host species! Auxotrophic selection Another option is to use a different...different method of selection entirely. Auxotrophic selection relies on the host organism being unable to synthesize... example, in both S. cerevisiae and E. coli. Auxotrophic selection is especially common in yeast experiments...spontaneously develop resistance to selection toxins. Auxotrophic selection is useful for producing stable mammalian...

...  Historically, ICSS was done by implanting an electrode and non-specifically electrically stimulating ...opposite chamber) [2]. Laser activation is usually controlled automatically using motion capture software such...apply. Testing stimulation First, In lieu of electrophysiology, it would be convenient to know if you are...targeted brain region after stimulation compared to controls without Chr2. c-Fos is a reliable and widely used...easiest way to resolve these issues is with electrophysiology. Concluding thoughts and moving beyond the...PMC4181379. 3. Kheirbek, Mazen A., et al. "Differential control of learning and anxiety along the dorsoventral ...silencer: development and application to optogenetic control of non-human primate cortex." Frontiers in systems...

...Under those optogenetic controls, gene expression levels reliably increased in vitro by a factor of 10 to...what the LITEs now enable. In a Nature paper introducing the optogenetic tool, Konermann and Zhang showed...populations. They demonstrated use of the LITEs to control gene expression in mouse neurons and in the brains...both quickly and significantly. Genes under LITE control showed a rise in transcription in as little as ...to establish it as “a novel mode of optogenetic control of endogenous cellular processes." The LITE system... Reference: Silvana Konermann et al. "Optical control of mammalian endogenous transcription and epigenetic...

...extremely strong affinity for one another. Actually the bond between them is one of the strongest noncovalent...that scientists began to exploit biotin and its strong interaction with avidin for molecular biology and...papers and webpages are listed below: EMSA (Electrophoretic Mobility Shift Assays) ELISA (Enzyme-Linked... biotinylate macromolecules of interest both in vitro and in vivo. Biotinylation reagents Biotin’s exposed...attached to a photoreactive arylazide group. Under strong visible light, the arylazide group becomes reactive... Krijgsveld, J., Katsantoni, E., Heck, A., … Strouboulis, J. “Efficient biotinylation and single-step ...Schwartz, S. A. “Biotinylated probes in the electrophoretic mobility shift assay to examine specific dsDNA...

...place the most important controls front and center, and make specialized controls available when needed....sequence and display frequently used genes and control elements. Development of this tool required creating... DNA sequence has changed. Identifying plasmid control elements Coding sequence features are relatively...relatively straightforward to define, but for control elements such as promoters and transcription terminators...obvious. We found that the annotated boundaries for control elements in commercial plasmids were inconsistent...how we use SnapGene during the plasmid quality control process Check out full plasmid sequencing at Addgene...

...or Salmonella, or any cells that bind strongly to nitrocellulose, over-incubation of plates can lead to...forcing DNA through a small opening using compressed nitrogen gas; this shears DNA at random locations and allows...containers that can easily be attached with a hose to a nitrogen gas tank with a pressure regulator. Other methods...cells, isolated colonies will be lifted onto nitrocellulose membranes and exposed to IPTG in order to induce...release recombinant proteins directly onto the nitrocellulose for subsequent probing for cell-binding activity...First, cut out 20 Petri dish-sized circles of nitrocellulose membrane and press each gently down onto a ... helpful to spot this protein directly onto nitrocellulose and carry out the steps from blocking the membrane...

...1980s, gene targeting technology was invented to introduce specific changes into the mouse genome. With these... early technologies, a researcher would first introduce a mutation into a mouse embryonic stem cell (ESC...chromatogram showing the telltale signs of a mutation introduced by CRISPR. The sequence started clean and then...handling embryos ex vivo, the components can be electroporated into the oviduct of pregnant females, a technique...gRNA, Cas9, and donor oligonucleotide can be electroporated into the mouse zygote (Qin et al., 2015).  ...targeting. CRISPR tools were recently used to introduce a 25 kb human gene into the mouse genome. However...Goodwin LO, Morgan JL, Juan WC, Roca X, Ong ST, Bergstrom DE (2018) Viable Mice with Extensive Gene Humanization...

...on the left, your digested DNA (and undigested controls) are loaded at the top of the gel in wells positioned... uncut sample of each plasmid to help with troubleshooting if your digests don’t look as expected. Once...to insert. It is critical to set up a negative control ligation reaction with no insert DNA added. This...much less total DNA (<1ng) or if you are having trouble getting colonies, you might want to use higher ...to be very large (>10kb) you might want to use electro-competent cells instead of the more common chemically-competent...minimum, two transformations after a ligation: 1. Control Transformation containing the ligation mixture ...see any colonies, you should conduct a positive control to ensure that your transformation worked. You ...

...will continue to add more controls, and have recently released these controls: pAAV-hSyn-DIO-EGFP (50457...sensors Optogenetics Recombinases And more! Control AAV For many AAV experiments, we recommend validating...aliquots in AAV1, AAV2, AAV5, AAV8, AAV9, and AAV Retrograde. You can also request the specific serotypes ...entire collection! We are expanding our entire control AAV inventory to give scientists access to a wide...AAV5 and 112677-AAVrg): The EF1a promoter directs strong, ubiquitous expression. This is a Cre-dependent... Email us or check our website to access them! Controls pAAV-hSyn-hChR2(H134R)-EYFP (26973-AAVrg) pAAV-hSyn-hChR2...