We narrowed to 960 results for: comp

...ask your interviewers You’re interviewing the company as much as they are interviewing you, so come prepared...performance evaluations, professional development, and company culture.  It’s also a good idea to prepare 1-2 .... This is a chance to highlight your prior accomplishments and demonstrate your science communication ... Connect your prior work or a new idea to the company’s area of expertise. This has potential to make ...consider bringing your own laptop as a backup, if the company will allow it. Before you start your presentation...interview. Expect to be tired once the interview is complete Industry interviews are long! Mine lasted 4.5 ...read emails from HR carefully because sometimes companies have relaxed dress codes for interviews. You can...

...your product’s web page, or in the company’s FAQs? A lot of companies and organizations selling biological...answering help emails and phone calls, I feel compelled to share my years of accumulated wisdom so that...information, but it’s not in the best interest of any company to hide information about their products from you...which case, you should probably stop using that company). Frequently asked question pages on website are...customers (and I expect this is the case at most companies). Always go back to the source. Although many...experience: Make sure you’re contacting the correct company. This might seem like a no-brainer, but it happens...please share the data. Maybe not all, but many bio companies have real scientists answering their technical...

...can we compare these methods in specific applications, we may also be able to use these comparisons to enhance...to 9 months in mice [2]). Low Immune Response: Compared to other viral vectors (e.g. Lentivirus, Adenovirus...is possible to use AAV constructs with self-complementing structure (scAAV) (7). As single stranded DNA...mapping of circuits In our lab, we use AAV-DREADD complexes to study neural function. Often the reporter proteins... proteins associated with these complexes are so robustly expressed that anatomical assessments can be...Ready-to-Use Retrograde AAV Preps at Addgene 2) Comparison of genetically engineered receptors (i.e. chemogenetics...neural control. Surprisingly, little research has compared and contrasted optogenetic and chemogenetic receptors...

...reaction or can be purchased 5’-modified from a company. However, unlike an RNA guide, it cannot be produced...probably while it is being synthesized) can form a complex with an ssDNA guide. Therefore, researchers must...inject cells with in vitro preassembled Cas9/gRNA complexes or with guide RNA- and Cas9-expression plasmids...survey and asked researchers if they had tested and compared NgAgo head-to-head with CRISPR-Cas9. The survey...respondents (transfection method, amount of each component per transfection etc.). Take Pooran's NgAgo Survey...same method used by Gao et al, 2016 (1). View the Complete and Up-to-Date Results of Pooran's NgAgo Survey..., leaving a small amount of active NgAgo/ssDNA complex in the cell. As proposed in the original article...

... a Type I-F CRISPR-Cas system. The four major components required for RNA-guided DNA integration are (... (Fig. 1A, top):  A CRISPR RNA (crRNA) comprising a 32 nt spacer sequence specifying the target site....sequences (R and L ends).  Figure 1: (A) Components involved in each type of CRISPR-Transposon system...multiple Cas proteins to form the Cascade targeting complex is a hallmark of Type I CRISPR-Cas systems, in ...nuclease activity. Instead, it forms a novel co-complex with the transposition protein TniQ for targeting... by the system. Guided by the crRNA, QCascade complexes survey the genome and bind target DNA sequences...pairing. Separately, TnsABC forms a transposase complex with the mini-transposon, excises it from the donor...

...library, as well as GC%, reverse compliment sequences, and more. Output_Compact: A text file that describes...there are also benefits gained by lowering the complexity of the screen. For example, the analysis becomes...a lasting impression on me. The slide showed a complex signaling pathway with many receptors, signaling...used these knockout mice to show exactly which components of the known pathway that were essential for ...be very useful for drug discovery, to dissect a complex signaling pathway, and to identify what part of...response to a particular ligand (such as when a component of a microbe or a cytokine is added to a cell)... rapidly performed by targeting all potential components of a signaling pathway. For example, one could...

...experts that complement your (scientific) skill set and is therefore very useful for complex projects. It...collaborations are becoming increasingly important as more complex approaches to scientific questions become popular...especially when considering small to mid-sized companies and organizations. Addgene as a nonprofit organization... worked with people outside the department to accomplish a variety of goals. For example, I worked with...provided a crash course in this discipline. Getting companies to come to these types of events can be very tough...First I needed to find the right contacts at the companies and then convince the contacts to give me some...repetition. I learned to look at the event from the company’s perspective, focus on the message and to keep ...

...function in unrelated host species, even from completely different kingdoms of life. That’s convenient... for researchers who want to do experiments in complex eukaryotic cells, but would prefer to grow, clone...replication begins, so a bacterial plasmid needs a compatible ori that will be recognized by its host’s replication...into plant genomes. Eukaryotes are a bit more complicated. Yeast vectors may contain an autonomously replicating...increase cellular uptake, but this approach requires compatible cell lines that express viral machinery. Alternatively...may be sufficient for your needs even without a compatible replication site. Regardless of the species you...vector to persist in... You need... Bacteria a compatible ori Yeast an autonomously replicating sequence...

...with fluorescent proteins (FPs) localized to compartments other than the cytoplasm. That is, unfortunately...positioning of the FP sequence relative to cell compartment targeting sequences, which often have absolute...that FPs are often poorly suited for cellular compartments other than the cytoplasm. Generally, FPs evolved...subcellular environments, including the organelles that compose the secretory pathway, endocytic vesicles, mitochondria.... Many of the resident proteins within these compartments undergo significant post-translational modifications...inter-chain disulfide bonds between FPs blocks Golgi complex-localized fusions from properly exiting the ER ...and spectral characteristics of the moxFPs are comparable to the non-optimized parental proteins. Our recent...

...GPCRs (Takeda S et al., 2002) and many downstream complexes involved, it can be difficult for scientists to...in sight (Olsen RHJ et al., 2020). A transducer complex consists of a trimer of three subunits: alpha, ...collectively known as a heterotrimeric G protein. This complex converts a signal received by a GPCR on the cell...is being able to identify specific transducer complexes that are activated in response to a ligand binding...inactive, these subunits are found in a trimeric complex bound to the intracellular surface of the GPCR....Following activation in response to the ligand, the complex will disassociate, resulting in a decreased GFP2...They further used TRUPATH to analyze a panel of compounds across the transducerome on a single GPCR, showing...