...broth and on the agar plates. Mycoplasma colonies are easily visible on the agar plates. This method is capable...detection, DNA staining, fluorescent tagging, agar plating, etc. Be wise to choose any of the techniques listed... (Uphoff and Drexler, 2011). Culturing on agar plates/broth is considered the ‘gold standard’ for detecting...prevent contamination rather than eradicating it later. Don’t be scared when mycoplasma infects your cell...

...genome-wide CRISPR inhibition Covalent tethering of DNA template to Cas9 SECURE base editors Nme2Cas9 CRISPR interference...inhibition library Covalent tethering of DNA repair template to Cas9 Homology-directed repair (HDR) can be ...using a single-stranded oligodeoxynucleotide HDR template covalently attached to the Cas9-gRNA RNP complex...Candida albicans A new CRISPR interference (CRIPSRi) platform for targeted gene repression in Candida albicans...

...like manipulations, anomaly, and so on, differently than scientists would. For example, “manipulate” could...different topics of science, especially disease related topics. Neurodegenerative and brain disorders are...illicit tampering” to general audiences while “manipulate” could mean “worked with” or “changed” to scientists...

...said. “It opens a new avenue of modifying and manipulating endogenous eukaryotic RNA.” Endless possibilities...Biological Engineering in March is fused to a translational repressor. In principle, however, designer PUF...PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1...(1):7. doi: 10.1186/1754-1611-8-7. PubMed.  Related Resources Addgene's Synthetic Biology Overview More...

...Expanding your network of relationships early and often is the most effective tactic a scientist in training...result of existing professional (and personal) relationships. However, most job seekers will and should apply...after the academic bench. Utilizing existing relationships as part of the job application process can dramatically...you can use alumni databases, friends, and even relatives to network your way to a hiring manager. Learn...

...to detach from the constant stream of thoughts related to lab research and gain fresh perspectives. Such...encourages exploration of tangents or seemingly unrelated ideas. It's beneficial to carry a notebook at ... identify areas in need of improvement. These unrelated ideas might hold the key to unlocking new perspectives...everything is a miracle.” This quote encourages us to contemplate our approach to life and how our viewpoints influence...

...by an inexpensive and widely available imaging platform: ultrasound. Challenges of in vivo imaging with...water-dwelling photosynthetic bacteria and archaea to regulate their buoyancy. The Shapiro lab recently discovered...ultrasound imaging. Ultrasound is an attractive imaging platform for in vivo imaging experiments because  ultrasound...imaging to show where the bacteria localize in relation to internal organs. The limit of detection for...

...opportunities for investigating genetic mechanisms related to morphogenesis, cell fate specification, and ...a conformational change of the receptor which modulates the fluorescence intensity of the cpGFP without...agrochemical compounds that have the potential to regulate the parasitization of cereal crops. As a result...activity on NAG PAMS and reduced activity on NGG PAMs relative to wild type. A new genome-wide CRISPR knockout...

... live animal that can be correlated to behavior, the technique is relatively slow and is not suited to...activated and inhibited neurons in live mice Report modulation of DA signaling during learning and reward consumption...Petzold, and Paul Greengard. "Dopamine-sensitive adenylate cyclase in caudate nucleus of rat brain, and its...

...the use of light sensitive proteins (opsins) to manipulate cell activity, enables researchers to silence...neuron more positive or more negative.  Neuronal stimulation: Depolarization When the inside of the neuron...the overlap between groups.  They found that photostimulating the Kolmer-Agduhr cells was sufficient to ...Kolmer-Agduhr cells on one side of the body required photostimulation in order to drive the full symmetrical response...

...system with your homologous recombination (HR) template, you could then: Confirm your HR by doing Restriction...2013 Science paper). If you detect positive HR, isolate single-cell colonies, grow them up, then perform...screen for positive ones.  -- OR -- If your HR template has a selection marker such as puromycin, you ... Biotech, 2 March 2014. Or browse the articles related to the most frequently requested CRISPR plasmids...

...) from the marine invertebrate Branchiostoma lanceolatum in 2012. Using structural modeling and structure-guided...which had a total of 31 amino acid replacements relative to CFP484. Because the excitation and emission...combination results in a 2.6-fold increase in signal relative to mCerulean imaged with a standard CFP set. mTFP1...fluorescent protein derived from Branchiostoma lanceolatum." Nature methods 10.5 (2013): 407. PubMed PMID...

...brain science, my curiosity did not ultimately translate to a desire to do the research itself. Several... neuroscience was carried over and allowed to percolate within a new intellectual environment. Over time...perception, scientific uncertainty and precautionary regulation was all necessary to inform the experimental ... health policy implications arising from sport-related concussion.   More resources on the Addgene blog...

...multiple, small colonies After streaking LB agar plates, you may notice that there are both large and small... bacterial cultures with lentiviral vectors, the flat white colonies contained the full plasmid, whereas...preps. In some cases, we suggest gel extraction to isolate the full, intact plasmid. Please see our gel extraction...recombination of plasmid DNA without affecting mRNA translation efficiency or half-life. RNA 25:507–518. https...

... encode spikes found on all of the different circulating variants. This allowed us to create pseudoviruses... the vaccine, we've been able to measure their relative ability to neutralize.”   Figure 2: Sera... shocked [with these results] because we were simulating the live virus experiment,” says Balazs. “But...resources on the Addgene blog Browse all COVID-19 related blog posts Learn more about pseudotyping Resources...

[email protected] Congratulations to the Golden Stopwatch awardees! Congratulations to all of the winners...faster with technology transfer offices (TTOs). The latest of these efforts is the Golden Stopwatch Award ...Team! Why MTAs Matter Though they may seem like boilerplate drudgery to some scientists, MTAs are important—they...

..., increasing the impact of the work, and as a collateral benefit, increasing the impact of the specific...comments to “you” I can easily check if I would have formulated specific comment differently if I would be speaking...Root System Architecture of Arabidopsis HapMap population in Amsterdam. Currently, she is a postdoc at ...expression patterns in plants with enhanced sodium accumulation in their roots and (3) development of tools ...

...Careers has developed a web-based, career-planning platform called the Individual Development Plan (IDP) and...using multidisciplinary, collaborative, and translational approaches for scientific tool and technique... of the project. For example, how can future regulations impact your work? What design variables need ...Patent process Business Models Communication Regulatory knowledge Networking Blogging Writing...

...thought there was room for improvement and a year later it became quite clear that others thought the same... piñata burst, value was subjectively ascribed relative to the basic needs of each individual; the  community...newer tools may still prove somewhat useful for the latest NTT targeting Cas protein, Cpf1. Separately, on-target...tools allow the user to save the results and return later or share them. Community exclusivity: Different...sites, scores and the statistics that go into calculating them. For this, some online tools such as Cas-OFFinder...

...changes of non-target genes and RNA-seq of a nuclear (MALAT1) and cytoplasmic (CKB1) RNA target. Adapted from...area, as the image below shows:    This system ablates tumors just as effectively as standard CAR-T cells...epitopes on GFP Monoclonal antibodies can yield relatively low signal in immunoassays due to only having...tested hfYFP in expansion microscopy as well as correlative light and electron microscopy and observed high...