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We narrowed to 972 results for: Abo;

Showing: 751 - 780 of 972 results

  1. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...controls must be run in triplicate.  The nice thing about qPCR is that since it measures relative quantification...number, albeit within a range of RQ values as noted above.   In both applications, results can be affected...

  2. Pooled Library Amplifications

    Type
    Blog Post
    ...First, you’ll need to collect specific information about your library: the size of your library (How many...with Addgene. If you have any specific questions about designing your library amplification protocol, feel...

  3. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...of the Addgene Scientific Advisory Board. His laboratory at Columbia University is focused advancing scientific... Listen to Our Interview with Alex Chavez Learn about Sequencing Options for CRISPR Genotyping Cas9 Activators...

  5. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...cloning, transfection, or long-term storage. But what about those pesky situations where your plasmid yield ...Topic Page Learn How to Verify Your Plasmid Learn about Common Lab E. coli Strains Additional Resources...

  7. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...famously visited Congress to help increase awareness about the potential of gene therapy!). It would take another...muscular atrophy, neurodegenerative diseases and metabolic diseases are also underway and reported results...

  10. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...and detergents.  Organic Solvents  As mentioned above, organic solvents can be used to simultaneously ...permeabilizing with saponin. However, if you are not worried about washing away your target (for example, some cytoskeletal...

  11. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ... work. After all, a stick-figure internet comic about math probably didn't sound like a great idea to ...  Selecting the method Once you’ve answered the above questions, you can create your short list of options...

  12. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...therefore prevent cell death. One of the cool things about amp is that it's exclusively toxic to dividing cells...antibiotic selection. If you feel like multiple of the above antibiotics would work for you, they likely do! ...

  13. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...or the PNS, respectively.  Read on to learn more about these two new AAV capsids and how they can be useful...Important considerations when using AAVs Learn more about using AAV as a tool for gene expression in mammals...

  14. MXS Chaining

    Type
    Blog Post
    ...Resources on the Addgene Blog Read about Gibson Assembly Learn About Other Plasmid Cloning Techniques Plasmids...

  17. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ... activity, and much more. Read on to learn more about GFP, how scientists have evolved this versatile ...effects of alterations to this activity. Learn more about optogenetics at OpenOptogenetics and find optogeneic...

  18. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...distant regions. They also don’t give any clues about the types of neurons involved or whether their involvement... Viral Vectors Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

  19. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...your experiment, meaning you don’t have to worry about vector replication at all. In these cases, transient...place in. In many cases, you won’t need to worry about the expression of your gene of interest in both ...

  22. How to Be an Excellent Trainee

    Type
    Blog Post
    ...individuals who are polite, appreciative, and clear about their needs. Remember, your bench mentor’s training...prepared and take training seriously, as reviewed above. Offer to help clean up and/or make necessary...

  23. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...potential applications of Cas13a given what we know about its structure and function? For starters, Cas13a... Check Out Our CRISPR Featured Topic Page Learn about Anti-CRISPRs Multiplex Genome Editing with Cpf1 ...

  24. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ... expression systems. Keep reading to learn more about the Gateway cloning method and its advantages. An... on the Addgene Blog Try Out FastCloning Learn About Methylation and Restriction Enzymes Use Colony PCR...

  25. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...the pX330 plasmids used in the validation assay (above) and it is just as effective (Mashiko et al., 2014...Engineering with CRISPR Validate Your Genome Edit Learn about Non Homologous End Joining and Homology Directed...

  26. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...Lentiviruses are a powerful laboratory tool often employed to establish cell lines that stably express...Get Tips for Titering Your Lentivirus Learn All About Viral Vector Elements Learn How to Do Genome-Wide...
Showing: 751 - 780 of 972 results