We narrowed to 989 results for: IGH@
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TypeBlog Post...‘foldscope’ (Dr. Prakash, Stanford University). Right there on the floor outside the lecture hall. It ...addition to low costs, deep tissue penetration, and high spatial resolution are benefits of this technique...
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Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton
TypeBlog Post...approaches. Why researchers should have a mix of high-risk, high-reward and low-risk but reliable projects. ... -
Our Most Popular Blog Posts from 2019
TypeBlog Post...sacB counter-selection) tools used in cloning. 8. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1... Proteins 101: GFP Fusion Proteins - Making the Right Connection by Joachim Goedhart Guest blogger Joachim... -
Editor's Choice, August 2016
TypeBlog Post...experiences with CRISPR alternative, NgAgo. As you might expect with any nascent technology, researchers ...from Addgene Executive Director Joanne Kamens. I highly recommend taking a look at all of our posts as ... -
Modulate the Activity of 17 Signaling Pathways with One Kit!
TypeBlog Post...cancers are treated with drugs designed to hit them right where it hurts, the effects are often remarkable...that confer resistance to drugs,” in a rapid, straightforward, and scalable manner, Wood said. Thankfully... -
From our Table to Yours: An Inside Look at Lunch at Addgene
TypeBlog Post...Right from the start, when we founded Addgene nearly 10 years ago, lunch felt like a family meal. My ... we have a few unique lunchtime traditions that might be fun to incorporate into your own lab. Iron Chef-style... -
X-CHIME: Context Dependent Germline Knockout in Immune Cells
TypeBlog Post...systems enable gene knockout in immune cells in a high-throughput manner, but these systems have not been...also includes pXPR_071, a plasmid that leads to higher titer lentivirus preparations than the first generation... -
Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog Post...precise genome edits, the recombinases used must have high activity and specificity with low cross-reactivity...recombinase/reporter pairs were inactive, even at very high recombinase levels. These data show that multiple... -
London Calling - Addgene’s New Europe Office in London
TypeBlog Post...collaborators was extremely helpful, but with hindsight I wished I had gotten them from Addgene. I really...really struggled to get those plasmids with the right information and I believe that a lot of European ... -
Antibodies 101: Affinity Tags
TypeBlog Post...proteins from E. coli is generally more straightforward and of a higher purity than mammalian purifications... -
Early Career Researcher Toolbox: Free Online Molecular Biology Tools
TypeBlog Post...mapping and design (Figure 1). Benchling: While you might think of Benchling as an electronic lab notebook...Design (DAD): This tool designs overhang sets for high complexity Golden Gate Reactions Cloning ligation... -
The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place
TypeBlog Post...growing open repository of CRISPR screen data that might be able to help you out. What is the Open Repository... cross-interrogation of different datasets that might otherwise be hard to identify. Figure 2: ... -
New Viral Vectors - Fall 2024
TypeBlog Post...monkeys it actually targets neurons, and has 45-fold higher expression levels compared to AAV9. You can now...AAV-CAG-GFP packaged in AAV9-X1.1! We also have eight new AAV-PHP.eB vectors from the Jonathan Ting lab... -
Oh, The Places You Can Go: Careers in Science Communication - Science Writing
TypeBlog Post...about science communication careers, you’re in the right place. For this series, I’ll interview three science... a writing position and looking for someone who might take over. I like writing and science, so I took... -
Fluorescent Proteins 101: Fluorescent Biosensors
TypeBlog Post...signal. Reporter signals may be electrochemical or light-based, with luminescent and fluorescent reporters...transfer between the fluorescent proteins. FRET is highly amenable to biosensor creation, and custom FRET... -
Tips for Improving Your Next Manuscript
TypeBlog Post...leaders in the field of microbiology with multiple high-profile publications. My classmates consisted of...scientific manuscript is not prose; it is to be straight to the point. 3. Engage the reader Scientists ... -
The Blue Flame Award: Celebrating Addgene's Most Requested Depositors
TypeBlog Post...celebrate our depositing PI’s who have the most highly requested plasmids. Currently, there are roughbly... highly requested plasmids... -
The Stingy Scientist: How the Baby Gel Box Was Born
TypeBlog Post... I settled on using glass slides. With just the right amount of agarose, I could pour a very thin gel ...surface tension. It's a bit tricky to pour the right amount so the agarose doesn’t pour over the sides... -
Choosing a Good Mentor for Scientists
TypeBlog Post...period. While there are many aspects to choosing the right labs (see my webinar (updated link from 2020) on...Mentoring" Don’t ignore warning signs If lab members, neighbors or alumni give you information about the PI that... -
Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource
TypeBlog Post...(Neuroscience AntiBody Open Resource). We are delighted to be partnered with Dr. James Trimmer of the ...this grant. Dr. Trimmer has created thousands of high-quality antibodies/nanobodies for neuroscience research... -
Antibodies 101: Epitope Tags
TypeBlog Post..., is species compatible, and doesn’t come with a high background can be a huge challenge. Epitope tags...amino acids. FLAG tag has been used with moderate to high success rates across all applications. You can find... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog Post...We hypothesized that this double stranding step might not actually be necessary. Since the AAV genome ...following DNA extraction, the [+] and [-] strands might naturally associate forming a double-stranded species... -
Safe Port in a Storm...How Addgene is Weathering the Pandemic
TypeBlog Post...other reagents every day, and this crisis has highlighted the importance of access to quality materials... -
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides
TypeBlog Post... fused to mature fluorophores, and can bind and light up epitope tagged proteins in vivo. These probes...sites within the variable portions of heavy and light chains. Proceedings of the National Academy of Sciences... -
Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science
TypeBlog Post...how aggressive my sentences might sound. What helps me in keeping the “right tone” is to address the comments... -
Using Addgene's New Viral Service for Your Research
TypeBlog Post...for similarly controlling neuronal activity with light will be available soon. We’re also distributing ...controls to determine if your AAVs are getting to the right cells using your particular delivery technique and... -
Even more elegant: Single injection CRISPR/Cas9 in C. elegans
TypeBlog Post...protocol takes about 2-3 weeks but requires less than eight hours worth of hands-on time. Creating a modular... the SEC-based system is both simple to use and highly efficient for C. elegans genome modification. ... -
25 Work From Home Tips for Scientists
TypeBlog Post...great way to take a mental break, while meditating right before bed will help you prepare for sleep. At Addgene... Home. It’s normal and okay to feel overwhelmed right now, but hopefully these tips are helpful to you... -
Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System
TypeBlog Post... to address the effects of genetic variants in a high throughput manner. She helped develop a method that...shape and metabolism of leaves can be changed by light and temperature and leaf autofluorescence can interfere... -
Improving Transduction Efficiency with Exosome AAVs
TypeBlog Post...). They saw that these AAV particles come in a slightly different shape. These AAV particles are often...purified AAV vectors. They found that they have a higher transduction efficiency, i.e. the same number of...