We narrowed to 928 results for: INA
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TypeBlog Post...before you know your system is working as expected. Finally, for each gene you want to activate, you should...study in cis regulation and also suffer from an inability to easily deliver the appropriate isoform(s) of...
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Plasmids 101: Control Plasmids
TypeBlog Post...factors need to be controlled for in order to eliminate any alternative interpretation of the results.... shown in Figure 2, this was likely due to the original transfection conditions. Now that we have successfully... -
Five Popular Model Organisms
TypeBlog Post...actually two distinct populations of β-cells that originate in different regions of the pancreas and produce...1935914. PubMed Central PMCID: PMC453137. Menezes, Regina, et al. "From the baker to the bedside: yeast models... -
Five Popular Model Organisms, Part 2
TypeBlog Post...attractive features is its stability and easily obtainable growing conditions. As a facultative aerobe, ...regenerate complex structures such as limbs, the spinal cord, and internal organs including the heart, ... -
March for Science
TypeBlog Post...level you can click on your state in this map. Finally, the march is meant to inspire scientists to be...Environmental Science and Technology: Crossing The Imaginary Line The New York Times: A Scientists’ March on... -
Antibodies 101: Introduction to Immunofluorescence
TypeBlog Post...BioRender.com The first couple of steps are preliminary steps that are not exclusive to IF, but they ...Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ... -
Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping
TypeBlog Post...did not affect the function or efficiency of the original APEX (Lam et al. 2014). They named this mutant... technique with proximity labelling to validate binary protein-protein interaction as well as identify... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog Post... The diversity panel - Equity and inclusion The final and possibly the most important panel of the symposium...quantifying, and preventing this type of failure. Final thoughts The FOR Symposium Boston 2015 shined a ... -
Technologies Enabled by NanoLuc® Luciferase
TypeBlog Post... genes generated through CRISPR/Cas9 knock-in. Finally, NLuc is very inviting for endogenous protein tagging...27786307. PubMed Central PMCID: PMC5476805. 8. Inagaki, S., et al. (2017) Genetically encoded bioluminescent... -
Finding Your Science Policy Path
TypeBlog Post...with early career science policy groups, attend webinars, and learn more about the field. Read the CCST...accelerator for some hands-on guidance. Check out my webinar via the NC Science Policy student group SciPolPack... -
Sequencing Options for CRISPR Genotyping
TypeBlog Post...variable) Sequence-level data Low-throughput, inappropriate for heterogenous populations, low sensitivity...given the stricter sequence requirement at the terminal 3’ PAM site (Fu et al. 2013; Pattanayak et al.... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog Post... the annealed cr:tracrRNA is then mixed with recombinant Cas9 protein to form the ribonucleoprotein complex...the promoter region of the non-HDR allele. For N-terminal tagging, specifically searching for error-free... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog Post...use, including all information provided in the original reference library, as well as GC%, reverse compliment... that you have specified. Run your screen! Final note I am very grateful to the researchers letting... -
Antibodies 101: Flow Cytometry
TypeBlog Post...and fluorescent light sources. Visible light illuminates each cell as it passes through and scatters in...staining is quicker than indirect staining and eliminates potential non-specific staining that may arise... -
Typing CRISPR Systems
TypeBlog Post...The current classification system relies on a combination of sequence similarity, phylogenetic analysis..., & Ke, A. (2024). Exploiting activation and inactivation mechanisms in type I-C CRISPR-Cas3 for genome-editing... -
New Coomassie Protocol Video
TypeBlog Post...latest protocol video, "Coomassie Purity Stain of Recombinant Antibodies." Hot tip! This protocol can easily... -
Antibodies 101 Series on TikTok
TypeBlog Post...antibodies #molecularbiology #science #scicomm ♬ original sound - Addgene We'll be posting these animations... -
Addgene's 20th Anniversary Party
TypeBlog Post...even made an appearance during co-founder and CSO Melina Fan's words of welcome! Executive Director Chonnettia... -
The 12 Days of CRISPR
TypeBlog Post...prime editing systems by manipulating cellular determinants of editing outcomes🎵 On the 5th day of CRISPR... -
Overwhelmed? Take a Break With Our 5 Favorite Science Comics
TypeBlog Post...some time out every once in a while – just read Melina Fan’s post on lunch. Why should our new blog be... -
Twenty Years of Sharing Science!
TypeBlog Post...community, we've partnered with distributors in China, Korea, Japan, India, and Brazil. We've also created... -
The Codon Guide to Synthetic Biology
TypeBlog Post...probably the papers and textbooks. I’ve selected “seminal” studies for various categories, including CRISPR... -
SciComm with the Experts at Science in the News Part 1
TypeBlog Post...communication. Science in the News hosts a podcast, seminars, a blog, conferences, and more for non-scientists... -
Twelve Amazing Guest Blog Contributors!
TypeBlog Post... to selecting fluorescent dyes and ligands. Illuminating Choices Paul Heisig, who shared his flow cytometry... -
Introducing Addgene's Microbiology Pages
TypeBlog Post...us about it with our Suggest a Plasmid Form! Finally, if you would like to get the word out about a ... -
PEI Calculator for Planning AAV Packaging Transfections
TypeBlog Post...References 1. Choi, Vivian W., et al. "Production of Recombinant Adeno‐Associated Viral Vectors for In Vitro and... -
Depositor Week 2020 is Happening Now. Join us!
TypeBlog Post...science along? We’ll also take a look back on some fascinating Addgene history that you might not have known... -
Streaking for Single Colonies: The Streak Plate Challenge
TypeBlog Post... pic.twitter.com/mk44J4Gcj9 — Dave W will get vaccinated and still wear a mask. (@GermJuggler) February... -
Tips for Working in Industry & Success in Management: Interview with Crystal Shih
TypeBlog Post... Shih is an Investigator III at Novartis, a multinational pharmaceutical company based in Switzerland.... -
Countdown to Halloween @Addgene
TypeBlog Post...suggestions for this year’s teams that may be procrastinating choosing their costume? As our organization...