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We narrowed to 928 results for: INA

Showing: 751 - 780 of 928 results
  1. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...before you know your system is working as expected. Finally, for each gene you want to activate, you should...study in cis regulation and also suffer from an inability to easily deliver the appropriate isoform(s) of...
  2. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...factors need to be controlled for in order to eliminate any alternative interpretation of the results.... shown in Figure 2, this was likely due to the original transfection conditions. Now that we have successfully...
  3. Five Popular Model Organisms

    Type
    Blog Post
    ...actually two distinct populations of β-cells that originate in different regions of the pancreas and produce...1935914. PubMed Central PMCID: PMC453137. Menezes, Regina, et al. "From the baker to the bedside: yeast models...
  4. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...attractive features is its stability and easily obtainable growing conditions. As a facultative aerobe, ...regenerate complex structures such as limbs, the spinal cord, and internal organs including the heart, ...
  5. March for Science

    Type
    Blog Post
    ...level you can click on your state in this map. Finally, the march is meant to inspire scientists to be...Environmental Science and Technology: Crossing The Imaginary Line The New York Times: A Scientists’ March on...
  6. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...BioRender.com   The first couple of steps are preliminary steps that are not exclusive to IF, but they ...Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...
  7. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ... The diversity panel - Equity and inclusion The final and possibly the most important panel of the symposium...quantifying, and preventing this type of failure. Final thoughts The FOR Symposium Boston 2015 shined a ...
  8. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ... genes generated through CRISPR/Cas9 knock-in. Finally, NLuc is very inviting for endogenous protein tagging...27786307. PubMed Central PMCID: PMC5476805. 8. Inagaki, S., et al. (2017) Genetically encoded bioluminescent...
  9. Finding Your Science Policy Path

    Type
    Blog Post
    ...with early career science policy groups, attend webinars, and learn more about the field.  Read the CCST...accelerator for some hands-on guidance.   Check out my webinar via the NC Science Policy student group SciPolPack...
  10. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...variable) Sequence-level data Low-throughput, inappropriate for heterogenous populations, low sensitivity...given the stricter sequence requirement at the terminal 3’ PAM site (Fu et al. 2013; Pattanayak et al....
  11. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...use, including all information provided in the original reference library, as well as GC%, reverse compliment... that you have specified.   Run your screen! Final note I am very grateful to the researchers letting...
  12. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...and fluorescent light sources. Visible light illuminates each cell as it passes through and scatters in...staining is quicker than indirect staining and eliminates potential non-specific staining that may arise...
  13. Typing CRISPR Systems

    Type
    Blog Post
    ...The current classification system relies on a combination of sequence similarity, phylogenetic analysis..., & Ke, A. (2024). Exploiting activation and inactivation mechanisms in type I-C CRISPR-Cas3 for genome-editing...
  14. New Coomassie Protocol Video

    Type
    Blog Post
    ...latest protocol video, "Coomassie Purity Stain of Recombinant Antibodies."  Hot tip! This protocol can easily...
  15. Antibodies 101 Series on TikTok

    Type
    Blog Post
    ...antibodies #molecularbiology #science #scicomm ♬ original sound - Addgene We'll be posting these animations...
  16. Addgene's 20th Anniversary Party

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    Blog Post
    ...even made an appearance during co-founder and CSO Melina Fan's words of welcome! Executive Director Chonnettia...
  17. The 12 Days of CRISPR

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    Blog Post
    ...prime editing systems by manipulating cellular determinants of editing outcomes🎵 On the 5th day of CRISPR...
  18. Twenty Years of Sharing Science!

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    Blog Post
    ...community, we've partnered with distributors in China, Korea, Japan, India, and Brazil.  We've also created...
  19. Countdown to Halloween @Addgene

    Type
    Blog Post
    ...suggestions for this year’s teams that may be procrastinating choosing their costume?  As our organization...
Showing: 751 - 780 of 928 results