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We narrowed to 1,010 results for: RING;

Showing: 261 - 270 of 1010 results

  1. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...of choice is AAV, with nearly every experiment requiring AAV. Before joining my lab, I had never worked... a heated stir plate to dissolve and requires monitoring so that the solution doesn’t get too hot because.... Rather than investing all of this effort in preparing reagents, I choose to only harvest the cell pellet...layered and then the viral suspension generated during AAV production is overlaid. Following ultracentrifugation...first purification. This helped me get a feel for layering the iodixanol solutions and let me double-check...make one or two small aliquots to use for qPCR titering. This helps avoid multiple freeze/thaws of your...of virus. AAV titration by qPCR Overview: AAV titering by qPCR quantifies the number of genome-containing...

  2. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...path began to take shape, was when I started volunteering. I wasn’t sure yet what a career in science ...volunteered at a place that made me happy. I started volunteering in the “Dinosphere” almost every week. I gained...dinosaurs, and met wonderful people. When I started volunteering, I was worried about becoming one of those angry...t sure what I wanted to do. A few weeks into volunteering, I was talking to a little girl about fossils...Beyond the outreach experience I gained from volunteering, it gave me a place to go once a week to get...before founding CINSO. Now, the network I built during graduate school is my informal advisory board. ...to share valuable life lessons they have learned during their scientific training and wrote about them ...

  3. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...the palm of your hand. Although white light is entering your hand, only orange/red light can be seen exiting...optical section is achieved that boosts contrast by filtering out emission light from fluorophores higher in...as spinning disk confocal. However, due to the scattering nature of biological tissue, imaging more than...parallelization (7) have allowed for real-time monitoring of neuronal activity across substantial 3D volumes... allow for multi-view imaging can image thick scattering samples in their entirety, something that is ...excitation light which can lead to photobleaching when acquiring multiple images over a large axial range. 2-photon...overcome this (8). The recent development of tissue clearing techniques (9) now allows researchers to image...

  4. Tips for arabidopsis transformation

    Type
    Blog Post
    ...they begin flowering. It is important to perform the transformation not long after flowering begins. If...RFP in the seed (Emami, Yee and Dinneny, 2013). Preparing plants for transformation Whatever plants you ...you can cut off the primary inflorescence (the flowering portion of the plant) near the base of the stem...al., 2006. Tips: Don’t plate seeds too densely during selection: this will increase background growth... (2002) Arabidopsis : a laboratory manual. Cold Spring Harbor Laboratory Press. Available at: https://...). Additional resources on the Addgene blog Engineering the plant genome using CRISPR/Cas9 Using pSiM24...pSiM24 for simplifying plant genetic engineering Using arabidopsis in the classroom Resources on Addgene.org...

  5. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...clicking “Add Plasmid to this Article” (left) and entering a plasmid name,plasmid type, and plasmid description...the plasmid names and descriptions first before entering the data. This step associates an Addgene ID with...plasmids all built with the same backbone and only differing in the gene/insert. Pooled libraries are normally... should click the "Enter Data" button to begin entering the specifics of your plasmids. Figure 5: Click... plasmid information. Enter plasmid information During the remainder of the process, you will enter plasmid.... You can complete these pages sequentially by entering the appropriate data and pressing "Save and Continue...species that the gene or insert sequence came from. Entering this information will populate a list of possible...

  6. Viral Production at Addgene

    Type
    Blog Post
    ...customers. Details about our production protocols, titering methods, and quality control are described below... or through collaboration with viral vector manufacturing facilities, such as the University of Pennsylvania...are then aliquoted and stored at -80 °C. Titer Titering is either performed by Addgene or by the University...University of Pennsylvania Vector Core. In general, titering is performed by the facility that produced the...those achieved using the standard qPCR method.  Titering by the University of Pennsylvania Vector Core ...Purity Purity of AAV preparations is assayed by comparing the relative stoichiometric ratios of the viral...aliquoted, frozen, and stored at -80 °C. Titer All titering is performed on lentiviral preparations that have...

  7. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...system to partition themselves to each daughter cell during cell division. They don’t simply rely on chance...CBP), and a partition NTPase (Schumacher, 2012).  During cell division, CBPs bind to the centromere-like...replication that occurs for some types low copy plasmids during cell division simply does not allow for enough ...strategy has been successful in displacing, or “curing” the virulence plasmids from the bacterial pathogens... SR, Iredell JR (2017) Plasmid interference for curing antibiotic resistance plasmids in vivo. PLOS ONE...X, Wang D, Wang H, Feng E, Zhu L, Wang H (2012) Curing of Plasmid pXO1 from Bacillus anthracis Using Plasmid...22253811 Ni B, Du Z, Guo Z, Zhang Y, Yang R (2008) Curing of four different plasmids in Yersinia pestis using...

  8. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...appeal, instant sharing across multiple other sites), I created a new platform for sharing video abstracts...University and developer of wesharescience.org. Sharing your research with the world can be challenging...printed journal articles as the dominant tool for sharing ideas. This leads to everyday obstacles for many...www.WeShareScience.org. WeShareScience is a free platform for sharing short, 3 to 5 minute videos about research. Videos...oceanographer who has a blog and several videos on sharing your research through video. Liz Haswell and ... 525.7569 (2015): 306-307. 3. Spicer, Scott. "Exploring video abstracts in science journals: An overview...Scientific Impact Data Freedom: The Expansion of Data Sharing in Research Publications Resources at Addgene....

  9. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...the questions and preparing answers early in the day. Although I didn’t start answering until 5pm, frankly...well moderated. One of our original worries about sharing science advice on Reddit was that we’d be dragged... our AMA and Joanne’s preferred time to start answering questions. r/Science has pretty strict rules about...straight on through her designated two hours for answering questions. She was able to do this because we ...posted at 8:00 am and Joanne didn’t have to start answering until 5 pm). The ability to see the questions ... Tips: Online Social Networking for Scientists Sharing Your Lab Protocols: Using Apps to Save Time & Track...Experiments Additional Resources See How We're Sharing Science through Video with the Addgene Youtube ...

  10. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...culture has become overly confluent or too old. Ensuring that you are working with healthy, happy cells... the lentiviral stock needs to be titrated. If storing for less than a day, lentivirus can be kept at ...dry ice/ethanol bath or liquid nitrogen prior to storing. Know your target cells Labs tend to follow the...titer the virus (read this blog post for tips on titering your lentiviral prep!) and infect. While this ...consider is how the virus is being titered. Most titering approaches transduce into highly permissive cell...receptors, other important binding events take place during the course of infection. These binding events can...23589850. 9. Cronin, J., Zhang, X.Y., Reiser, J. “Altering the tropism of lentiviral vectors through pseudotyping...
Showing: 261 - 270 of 1010 results