We narrowed to 911 results for: TRO

...etiopathomechanisms of ischemic stroke, the most common type of stroke and the third leading cause of ...-Seeding Labs plasmid grant we are excited to introduce the 5 awardees and their research. These researchers... is necessary to improve tuberculosis care and control. In her current research, Sheen aims to identify...taraxacifolia (Willd), an annual herb of Western Tropical Africa typically known as wild lettuce. Aside ...of death and disability worldwide. An ischemic stroke occurs when arteries leading to the brain become ...of interesting combinations: biomedicine and astrobiology. During the last 11 years he has studied various...

...The calcium is strong here….I can sense it….sound like a plot to an biology scifi movie? Or maybe it’...neurotransmitter, such as norepinephrine, or an electrolyte such as calcium. This varied component, which...For one, many viral platforms, such as AAV or retrovirus, can either permanently integrate and/or express...serotypes can be used to affect or determine viral tropism—the type of cells the viral particles infect. This... sensor, whether you choose lentivirus, gamma retrovirus, or AAV as your vehicle, we’ve got you covered...Resources References  Bhalla N., Jolly P., et al. Introduction to Biosensors. Essays Biochem. 2016 Jun 30;60... Susanna Stroik...

...you can select an appropriate isotype control. An isotype control has the same constant heavy chain domain...heavy-chain constant region (see Antibodies 101: Introduction to Antibodies for a refresher). There are five...molecules are recruited by the antibody to help destroy and remove a pathogen. Different isotypes also ...doesn’t bind your antigen of interest. Isotype controls are used to make sure the staining you see is ... than nonspecific background staining. Isotype controls are used in antibody-based applications including...techniques that you can use antibodies for in our Intro to Antibodies post Check out our Antibodies 101:...

...generation lentiviral packaging plasmid from the Didier Trono lab that expresses the HIV gag, pol, rev, and tet...pMD2.G. Find psPAX2. pMD2.G - Also from the Didier Trono lab is pMD2.G, a VSV-G envelope expressing plasmid...envelope protein for producing lentiviral and MuLV retroviral particles. It can be used with packaging plasmids...as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral). Fun fact: published in 2003, this plasmid predates...generation lentiviral packaging plasmid from the Trono lab contains the rev gene. It’s meant to be used...generation lentiviral packaging plasmid from the Trono lab that contains the gag and pol genes. It also...shRNA. This scrambled shRNA is used as a negative control for mammalian RNAi experiments.  Find the scrambled...

...consequently the principal means by which CRISPR/Cas9-introduced breaks are repaired. The following factors are...ends tends to be the point where mutations are introduced. Repair of Cas9-induced breaks by NHEJ As illustrated...microhomologies”). NHEJ doesn’t obligatorily introduce indels. Given the end structure of the Cas9 DSB...hundreds of base pairs or more can simultaneously introduce a pair of chromosome breaks, and could result ...single strand break; when two such breaks are introduced near each other, in opposite strands. This “double...j.cell.2013.08.021 Waters CA, Strande NT, Pryor JM, Strom CN, Mieczkowski P, Burkhalter MD, Oh S, Qaqish BF...Video] Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens        ...

...phosphorylated DNA termini to 3 ́-OH DNA termini (1-4). In vitro, ligases (notably T4 DNA ligase) are critical reagents...circularization of dsDNA (6). Less commonly utilized in vitro, Taq DNA ligase will ligate only nicks (5-8). Taq...identifiable and quantifiable by capillary electrophoresis (CE) (see below). This method allowed screening...gene PCR-ligase detection reaction-capillary electrophoresis assay." Journal of clinical microbiology 45.6...PMC1933048. 16. Cheng, Changming, et al. "Electrochemiluminescent assay for detection of extremely rare mutations...mixed population of excess wild‐type DNA." Electrophoresis 34.9-10 (2013): 1415-1422. PubMed PMID: 23463388... PMID: 17466627. 25. Luo, Jianying, Donald E. Bergstrom, and Francis Barany. "Improving the fidelity of...

...that antibody works as expected, you will see a strong antibody signal in the retinal sample, but little...you see a similar antibody signal between your control and your knock-out sample, you should be concerned...antibody binding in the transfected cells but not in control cells. But (there is always a ‘but’), you should...encoding this protein, which we will call Negative Control Gene (NCG) (A). Then you lyse the cells and run... your antibody (B). Your results show a lovely, strong band from the cells transfected with your protein...well as a third unrelated antibody as a negative control (A). The two antibodies to your protein of interest...sample using the antibody and then uses mass spectrometry to identify the captured proteins. If the antibody...

...anticipated outcomes? What are your controls? Will these controls enable you to interpret your results...e.g. an R01 from the NIH, in the United States) Strong letters of reference that attest to your talent...figures are missing? For example, are additional controls necessary? As you build your story, are additional...person makes time for you, as needed, this is a strong indicator that this person cares about your science... together, all of this means that you need to introduce yourself to and talk with more senior scientists...you and your research exposure. Use meetings to introduce yourself to and interact with the members of your...Medical Institute. His interests include the quality control of secretory proteins in the Endoplasmic Reticulum...

...identified seven genera in in vitro experiments, possibly because in vitro conditions did not optimally...situ Conjugation (MAGIC). With MAGIC, scientists introduce an Escherichia coli donor strain into the microbiome...antibiotic selection. The team tested this system in vitro and in vivo. They identified cells from 19 genera...from different bee species. BTK can be used to introduce fluorescent markers to visualize localization ...protein. The expression of this fusion is under control of the arabinose-inducible promoter. Cells are ...showed that this method can be used for both in vitro and in vivo studies. Find distributed cell division...

...eliminate fluctuations due to copy number by introducing genes of interest into the chromosome. But this...input signal that both positively and negatively controls the output. In this case, plasmid copy number ...gene increases its expression. However, if you introduce negative regulation by using a promoter that is.... placed a trans-acting RepA protein under the control of IPTG. Because RepA is a plasmid replication ...Segall-Shapiro et al. placed the genes under the control of a constitutive promoter and then moved them ...decreased. But when the genes were placed under the control of a stabilized promoter, deoxychromoviridans titers...the joys of a p-value < 0.05, having such fine control of gene expression is useful for many metabolic...