Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 954 results for: App;

Showing: 521 - 540 of 954 results

  3. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...biomolecules. They can be part of multiple dynamic and overlapping complexes that have distinct functions. Many .... (2017). Split-BioID a conditional proteomics approach to monitor the composition of spatiotemporally...S., & Varnaitė, R. (2016). Meet the neighbors: Mapping local protein interactomes by proximity‐dependent...

  4. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ..., non-enveloped virus that carries a small, approximately 4.7 kb single stranded genome. AAV belongs to... over a period of 1 to 3 years – the treatment appears to be both safe and effective. Challenges for AAV...trials support the utility of AAV as a broadly applicable gene delivery tool, aspects of AAV biology should...

  5. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...protein in your sample? Or what if something weird happened during your western and your results look… funky...to probe the blot again, almost like new. This approach preserves your sample and saves you from having...retaining residual antibodies. If you have already applied antibodies and need to dry the membrane for storage...

  6. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...multigene constructs, through multi-TU assembly,happens via three steps: Level 0: Cloning of basic parts...multi-purpose it can be. Let’s review a list of MoClo applications. Genetic circuits Most things in biology are...phenotype is best described with “it’s complicated.” Applying MoClo Interested in using MoClo? You don’t have...

  7. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ...more about it on the blog. COVID-19 research is happening fast and we’re glad to join these efforts and ... JM, DiVenere AM, Kuo H-C, Javanmardi K, Le KC, Wrapp D, Lee AG, Liu Y, Chou C-W, Byrne PO, Hjorth CK,...resources on the Addgene blog Learn more about mapping the SARS-CoV-2 - human protein-protein interaction...

  8. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    ...of competitive TED talk – to win the audience’s applause with your performance. When your institute opens...guided tours to show people where the lab magic is happening. 3) Volunteer – Step into science education Do... Blog - Hone your writing skills Take the DIY approach and start a blog. Stellar talents like Ed Yong...

  9. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...Addgene AAV Production protocol, I take a lazier approach for harvesting my AAV. I skip the PEG precipitation...the PEG will separate into two phases. If this happens, the solution can be cooled and the two phases ...immediately before iodixanol purification. This approach gives me a bit of flexibility since I can store...

  10. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ... original CAPTURE and CAPTURE 2.0 methods.   Applications of CAPTURE 2.0 CAPTURE 2.0’s increased rate ... of chromatin interaction networks  A second application for CAPTURE 2.0 is the construction of spatial...temporal changes in chromatin interactions A third application for CAPTURE 2.0 is detection of temporal changes...

  11. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...Cascade binding and subsequent DNA degradation appears to be conserved. This DNA degradation portion can... portion of DNA using only a single gRNA. Cas3 appears to have fewer off-target alterations, likely due...Mashimo, T. (2022). Genome editing technology and applications with the type I CRISPR system. Gene and Genome...

  12. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...machine learning (PRIDICT, PRIDICT2.0).   How to apply PRIDICT in prime editing experiments Prediction ...pegRNA efficiencies with pridict.it. This approach leverages the strengths of PRIDICT/PRIDICT2.0 ... computational biology. His expertise includes applying machine learning techniques to make sense of biology...

  13. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...The CRISPR corner New ready-to-use viral preps Mapping antibody escape in SARS-CoV-2 by mutational scanning... sequencing. From Starr et al Cell., 2020. (B) Mapping antibody-escape mutations with the yeast display...specific anti-SARS-CoV-2 antibodies (Fig 1B). This approach can augment viral surveillance by enabling rapid...

  14. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    ... map the internal organization of a cell. This mapping can be done by using antibodies targeted to the...meaningful way it is important to choose the appropriate quantification methods and tools. It is usually...Pages 2. Cooper GM. 2000. The Cell: A Molecular Approach. 2nd Edition. 3.  CureFFI.org. Cell Biology 04...

  15. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...virtually any genetic locus when complexed with an appropriately designed small RNA, or guide RNA (gRNA). The...expression with truncated gRNAs An alternative approach is to modulate Cas9’s nuclease activity by modifying...engineering parameters via a library-on-library approach." Nature methods 12.9 (2015): 823-826. PubMed ...

  16. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...of proteins. But they are still flat. To truly appreciate the 3D structure of these proteins, it is necessary...molecular viewing software.   In reality, this rarely happens. But if you ever have an opportunity to read one...invariably say something like “Oh,…I didn’t quite appreciate that….” For example, when Tom Steitz was presented...

  17. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...are, you’ll even have DNA left over for other applications, like PCR, cloning, transfection, or long-term...wash step or use a different strain. For most applications, Addgene uses and recommends DH5𝝰! This strain...your E. coli to ensure it is suitable for your application. We have previously reviewed many common lab ...

  18. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...(2015). “A Scalable Genome-Editing Based Approach for Mapping Multiprotein Complexes in Human Cells.” ...lies in the diversity of nucleases and their applications. It will be interesting to see what new editing...

  19. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...reference sequence into the Subject Sequence box that appears. Aligning two nucleotide sequences is probably ...frameshift mutation present in the ORF is readily apparent when viewing blastx results. When using blastx...webservices/#pairwise_sequence_alignment_psa Happy BLASTing! Do you have any tips for using BLAST to...

  20. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

    Type
    Protocol
    ...of interest (see below for recommend amounts) Appropriate restriction enzyme (see manufacturer's instructions...instructions for proper ammount) Approrpriate restriction digest buffer (see manufacturer's instructions) ...as Addgene's Sequence Analyzer . Determine an appropriate reaction buffer by reading the instructions for...The amount of DNA that you cut depends on your application. A diagnostic digest typically involves ∼500 ...volume usually varies from 10-50 µL depending on application and is largely determined by the volume of DNA...better. Mix gently by pipetting. Incubate tube at appropriate temperature (usually 37 °C) for 1 hour. Always...manufacturer’s instructions. Pro-Tip Depending on the application and the amount of DNA in the reaction, incubation...
Showing: 521 - 540 of 954 results