We narrowed to 572 results for: Cit

1. Hot Plasmids and Viral Preps - May 2021

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   ...Flp-dependent DREADDs viral vectors. Both inhibitory and excitatory DREADDS from the Gether lab are now available...

2. Antibodies 101: Conventional vs Spectral Flow Cytometry

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   ...example of the emission spectra for two fluorophores excited by the violet laser, BV421 and BV711. These “rainbow...

3. Addgene's Cancer Collection Pages Connect the Research Community

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   ...residues. For Ras researchers, one of the most exciting and relatively new resources is the RAS Pathway...

4. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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   ...CFP, YFP, BFP, Cerulean, Turquoise, Venus, and Citrine) can be monomerized with an A206K mutation [2]....

5. Genome-wide Screening Using CRISPR

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   ...over previous genome editing techniques is its simplicity and versatility. CRISPR/Cas9 consists of two ...

6. AAVs CREATed for Gene Delivery to the CNS and PNS

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   ...long-term expression; and 3) they have limited toxicity. However, a key challenge of using AAVs for neuroscience...

7. Simplify Cloning with in vivo Assembly

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   ...molecular biology of synaptic transmission and plasticity.     Javier García-Nafría is an investigator ...

8. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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   ...the Brunello knock-out library while mitigating toxicity induced by high copy number dsDNA cutting. A Dolcetto...

9. Transferable Skills Guide: Identifying Your Transferable Skills

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   ...satisfaction out your work, and be prepared for new and exciting opportunities. Here are some strategies for identifying...

10. Viral Vectors 101: The Retroviral Lifecycle

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    ...mouse embryo cell lines in which each cell has the capacity to be activated to produce infectious murine leukemia...

11. Uncovering Ancestral Bias in CRISPR Research

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    ...their associated ancestries. While there were some exciting relationships identified, 48% were found to be...

12. DNA Purification Without a Kit

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    ...stacked together in series to increase binding capacity. Figure 1: Purify DNA using glass filters...

13. Tips for Screening with Yeast Two Hybrid Systems

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    ...eukaryotic transcriptional activator bearing the DNA specificity of a prokaryotic repressor. Cell. 1985; 43:729...

14. The Importance of a Fun Workplace: Company Culture at Addgene

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    ...your event in casual conversation to get people excited!    Laura Veckerelli is the Administrative and...

15. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    ...proteins and CRISPR became available, I was very excited because genome-editing with these molecules makes...

16. CRISPR 101: Any Base Transversion Editors

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    ...tool vary considerably. And there’s so much more exciting protein engineering going on in the base editing...

17. Fluorescent Proteins 101: Aptamer Fluorophores

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    ...molecules, which could bind their targets with high specificity and affinity, were eventually called aptamers...

18. Mesothelioma - Causes, Symptoms, and Treatment

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    ...spread beyond its point of origin. One of the most exciting developments recently is a new blood test for ...

19. Transferable Skills Guide: External Collaborations

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    ...Partnership Management, a very interesting and exciting part of my work. However, this is not the first...

20. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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    ...Creating PHP.B, a neurotropic AAV9 variant PHP.B’s specificity for C57BL/6J mice is likely due to how the Deverman...