Meet the New Addgene Board Members!
Type
Blog Post
...high school, I knew that this is what I wanted to do,” Stegmeier says
He began this journey in biochemistry...
We narrowed to 771 results for: Dos
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Celebrate Open Data Day with Addgene’s AAV Data Hub!
TypeBlog Post... the AAV data hub. How to share your AAV data Do you use Addgene AAV tools and want to join the ranks...methods. For example, you can provide location, titer dose (Example: 1E13 GC/mL) and subject age at time of... -
The Strength of Story Telling
TypeBlog Post... crucial skill for career advancement. Scientists do and should tell stories all the time. They provide... -
Sensing Neuronal Dopamine
TypeBlog Post...learning, decision making, and motor control. What do we understand about Dopamine? DA is produced by specific... -
Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight
TypeBlog Post... rhythmic movements like spontaneous swimming. To do so, the lab expressed the LiGluR opsin in different... -
Running for Rare Disease, Running for FOP, Running for AJ
TypeBlog Post... know, treatments that work well in animal models do not always work as efficiently in humans. This is... -
Year of the Camelid: Antibody Style
TypeBlog Post...of different cancers (Jin, et al. 2023). So where do all these sdAbs come from? In the early years of ... -
Understanding GPCR Signaling Using TRUPATH
TypeBlog Post...able to detect extracellular stimuli. One way they do so is through G protein-coupled receptors (GPCRs)... -
The Golden Stopwatch Award: Recognizing Facilitators of Research
TypeBlog Post...why MTAs matter, check out another blog post: Why Do I Need an MTA Anyway?) Don't want to wait? Talk with... -
Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio
TypeBlog Post...and excitement about how much more there is left to do with the characterization and implementation of this... -
Choosing Your Perfect Empty Backbone
TypeBlog Post...track your protein in the cell or purify it – and you do not have a specific antibody against the protein ... -
Plasmids 101: Antibiotic Resistance Genes
TypeBlog Post...vary in their sensitivitly to heat and/or light – do not add them to media hotter than about 55oC and ... -
Extracurricular Activities for a Strong Science Career Path
TypeBlog Post...education and mastering new skill sets must occur by doing work outside of the lab. Extracurricular activities... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog Post...efficient editing. Repair oligos do not need to be PAGE purified, although doing so increases knock-in efficiency... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog Post...cofactor? How many binding partners does it have and how do they come off and on in a cellular environment? If...can undergo FRET when they are near each other. How do you label your biomolecule of interest with FRET ... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog Post...most important question to consider is what exactly do you want to use optogenetics for? The technique is... is non-trivial; you don’t want to spend months doing surgeries and making fiber optic cables only to ... -
Trading Spaces - Visiting Scientist Edition
TypeBlog Post...third parties or your home institute. Discuss rights Do you expect to generate data that will contribute ... -
Antibodies 101: Epitope Tags
TypeBlog Post...- but for many other proteins, quality antibodies do not exist, or they don’t exist for a specific application... -
Antibodies 101: Chimeric Antibodies
TypeBlog Post...indirectly probed simultaneously on the sample. What to do? If you’ve ever found yourself facing a similar ... -
The MTA at Addgene
TypeBlog Post...transparency in an otherwise messy process. And you do have options for the terms you want to share your...