We narrowed to 772 results for: Dos

...enveloped viruses bud out, instead of lysing, but they do generally still kill the infected cell.) This is ...

...where affinity tags come in – they are peptides which do not naturally exist in most expression systems that...

...both bioinformatics tools and knowledge databases. Do you have a favorite online molecular biology tools...

...CRISPR libraries As CRISPR technologies multiply, so do CRISPR pooled libraries! Addgene’s collection also...

...EasyTag System is a straightforward workflow for doing gene targeting, reporter generation, controllable...

...high school, I knew that this is what I wanted to do,” Stegmeier says He began this journey in biochemistry...

... the AAV data hub. How to share your AAV data Do you use Addgene AAV tools and want to join the ranks...methods. For example, you can provide location, titer dose (Example: 1E13 GC/mL) and subject age at time of...

... crucial skill for career advancement. Scientists do and should tell stories all the time. They provide...

... know, treatments that work well in animal models do not always work as efficiently in humans. This is...

...and excitement about how much more there is left to do with the characterization and implementation of this...

...track your protein in the cell or purify it – and you do not have a specific antibody against the protein ...

...vary in their sensitivitly to heat and/or light – do not add them to media hotter than about 55oC and ...

...education and mastering new skill sets must occur by doing work outside of the lab. Extracurricular activities...

...efficient editing. Repair oligos do not need to be PAGE purified, although doing so increases knock-in efficiency...

...cofactor? How many binding partners does it have and how do they come off and on in a cellular environment? If...can undergo FRET when they are near each other. How do you label your biomolecule of interest with FRET ...

...most important question to consider is what exactly do you want to use optogenetics for? The technique is... is non-trivial; you don’t want to spend months doing surgeries and making fiber optic cables only to ...

...experiments, it’s important to verify that these changes do not impact translation rate, as the authors of these...

...oligomerize when exposed to ultraviolet light. To do so, she generated fusion proteins in which Dronpa...

...also see how fun biology can be! What can BioBits do for you? We initially developed the BioBits kit to...

...eukaryotes don’t naturally have this response, they do contain the specific ubiquitin ligase pathway needed...