We narrowed to 825 results for: EED
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TypeBlog Post...assemble large DNA sequences, no restriction enzymes needed Not optimal for joining sequences with a high degree...similarly apply MXS chaining to your experimental needs. Let us know how you use MXS Chaining by emailing...
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Simplify Cloning with in vivo Assembly
TypeBlog Post...multiple recombination events, all linear fragments need to get into the same cell. For simple cloning requiring... errors during PCR: we recommend Phusion or Q5. Speed up your life Rather than making up your PCR mix ... -
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog Post...is the point? Antibodies are large proteins that need a little help crossing cell membranes as they are...the inside of the cell. However, assuming you do need to permeabilize, you have a few options for what... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog Post...the virus and isolation of the nucleic acid. Proceedings of the National Academy of Sciences of the United...pseudodiploidy and high rate of genetic recombination. Proceedings of the National Academy of Sciences, 87(4), 1556... -
Better Dyeing Through Chemistry & Small Molecule Fluorophores
TypeBlog Post...between the size of the genetically encoded tag, the speed and selectivity of the fluorophore attachment, the...protein. In some cases this property eliminates the need for removing excess dye from the sample, which is... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog Post...world, coordinates alone aren’t very useful. We’ll need something like Google Maps to help us identify and...NANOG enhancers from step 1 with genome math, we’ll need a bit of shorthand from the field of epigenomics... -
Cpf1: A New Tool for CRISPR Genome Editing
TypeBlog Post...one RNA rather than the two (tracrRNA and crRNA) needed by Cas9 for cleavage. In certain cases, Cpf1 may...that of the ~100 nt crRNA/tracrRNA hybrid guides needed for Cas9 function. Since both Cpf1 and its guide... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog Post...immediately transformed and stored. Some samples need to be transformed with a micropulser using an electric...samples require a reduced antibiotic concentration or need to be transformed into different cells than were... -
Hot Plasmids - August 2020
TypeBlog Post... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...bistable network, stripe pattern-forming incoherent feed-forward loop. Find the plasmids at Addgene! ... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog Post... noticed Holly Bik live-tweeting about topics I needed to know more about. I caught up with her outside...Some of the variability was organic, as some people need less assistance than others, but we did have some... -
5 Reasons to Use Reddit for Science Communication
TypeBlog Post...Submission Guidelines here, but essentially we just needed to send a Google calendar invite over to the r/...a paid account which gives me all the features I need to save, organize and easily find the resources ... -
Career Insights: Technical Support Specialist
TypeBlog Post...companies produce different products and therefore need people with different areas of expertise in technical...unobtainable solutions. To some extent there is the need (or opportunity) to get involved in product testing... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog Post...Ligand regulated recombinase - keeping Cre out until needed But what can be done when the recombination should...induced by Cre recombinase expression while proteins needed for later experiments (cellular tracking for instance... -
Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names
TypeBlog Post...in new ways. Along with a new location comes the need for new conference room names. The Addgenie’s favorite...understand where sonic hedgehog got its name, we need to go back to 1980, when Christine Nusslein-Volhard... -
CRISPR-mediated Plant Base Editors
TypeBlog Post... gene Y, which has a defective allele Ym and you need to convert an adenine (A) to a guanine (G)? You ...with the endogenous tRNA-processing system." Proceedings of the National Academy of Sciences 112.11 (2015... -
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog Post...host cell division to plasmid proliferation." Proceedings of the National Academy of Sciences 80.15 (1983... model for programmed bacterial cell death." Proceedings of the National Academy of Sciences 93.12 (1996... -
Neuronal labeling with Spaghetti Monster
TypeBlog Post... neuronal synapses for instance. Also these tags need to be fused to scaffold proteins in order to be ...composed of FPs fused to multiple epitope tags. Indeed, to create the smFPs Looger’s team strategically... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog Post...2014). Scientists from the University of Geneva (Creed et al., 2015) used optogenetics to support these...Psychoneuroendocrinology 46 (2014): 78-87. PubMed PMID: 24882160. Creed, Meaghan, Vincent Jean Pascoli, and Christian Lüscher... -
Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research
TypeBlog Post... adjacent motif) near the target DNA, TnpBs also need a short sequence motif called a TAM (transposon-... used to generate gene-edited plants without the need for tissue culture (Maher et al., 2020; Qiao et ... -
Hot Plasmids: Fall 2024
TypeBlog Post...processes in C. elegans: mScarlet3 for applications needing intense brightness (such as low-expression proteins...co-imaging. And because bioluminescent markers do not need to be excited by an external light source, they ...