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  1. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...system – HaloTag and its PROTAC are all that is needed to facilitate degradation. HaloTag is also the ...tag does depend a lot on your unique experimental needs. If your protein’s function is easily perturbed ...

  3. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...arm repair template (Fig. 1, Step 1). There is no need for PCR or cloning, as digestion of the destination...endogenous genes in human cells. To use CRISPaint, users need 3 vectors:  1) a gRNA vector that targets the gene...

  5. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...recognition, which gives a researcher unprecedented freedom to target any sequence of DNA. Second, NgAgo target... by a few respondents suggesting that NgAgo can indeed be optimized for genome editing in mammalian cells...

  6. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...all the time. Once, I had this crazy idea that I needed help with: wouldn’t it be fun to get several of... example: Don't call me a dropout: Why science needs more people to quit the lab. Resources on the Addgene...

  7. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ...pages contain all the detailed information you'll need to use the plasmid in your experiments: Backbone... plasmids).  We also like to store plasmids that need extra stability, such as some of our viral vectors...

  8. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...process has steadily grown. On a busy week, we may need to analyze more than 200 plasmids as part of our...suggestions? Share your thoughts here to help other labs speed up their plasmid and cloning verification steps ...

  9. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...emission wavelength range, there are other traits that need to be considered when choosing an FP: Unique categories...considered when labeling proteins that interact. Indeed, FRET is often used to determine if two proteins...

  10. MXS Chaining

    Type
    Blog Post
    ...assemble large DNA sequences, no restriction enzymes needed Not optimal for joining sequences with a high degree...similarly apply MXS chaining to your experimental needs. Let us know how you use MXS Chaining by emailing...

  11. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...the virus and isolation of the nucleic acid. Proceedings of the National Academy of Sciences of the United...pseudodiploidy and high rate of genetic recombination. Proceedings of the National Academy of Sciences, 87(4), 1556...

  13. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...multiple recombination events, all linear fragments need to get into the same cell. For simple cloning requiring... errors during PCR: we recommend Phusion or Q5. Speed up your life Rather than making up your PCR mix ...

  16. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...one RNA rather than the two (tracrRNA and crRNA) needed by Cas9 for cleavage. In certain cases, Cpf1 may...that of the ~100 nt crRNA/tracrRNA hybrid guides needed for Cas9 function. Since both Cpf1 and its guide...

  18. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ... neuronal synapses for instance. Also these tags need to be fused to scaffold proteins in order to be ...composed of FPs fused to multiple epitope tags. Indeed, to create the smFPs Looger’s team strategically...

  19. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...companies produce different products and therefore need people with different areas of expertise in technical...unobtainable solutions. To some extent there is the need (or opportunity) to get involved in product testing...
Showing: 521 - 540 of 816 results