We narrowed to 629 results for: EMB

1. Viral Vectors 101: An Introduction to AAV

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   ...expression for several years (Wonjo et al., 2013). Remember that AAVs do not integrate into the host genome...

2. The time and cost required to make a plasmid

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   ... is a concern. The faster methods, like Gibson assembly, are typically more expensive, so you’ll have ...

3. Addgene: The First Twenty Years

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   ...labhold” name. Our first plasmid request came on September 8, 2004, for pcDNA-p160MBP, while the first request...

4. Summer SciComm: Preprints

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   ...journal and share it more widely!  Not everyone embraces preprints as part of their scientific communication...

5. Antibodies 101: Affinity Reagents

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   ...through mutation, allowing diabodies to be used for assembling protein nanostructures. Diabodies readily co-...

6. Transferable Skills Guide: Problem Solving

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   ...both in and out of the lab. The next step is to remember that you have these skills when you start applying...

7. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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   ...multiplexed imaging of neurotransmitter, calcium, and membrane voltage,” says Tian. Figure 5: Citrine-tagged...

8. Gendered Innovations: Why Does Sex of the Cell Matter?

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   ...analysis for innovation and discovery. She is a member of the American Academy of Arts and Sciences and...

9. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

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   ... their ability to cut the EMX1 locus in human embryonic kidney (HEK) cells, 11 of these initial mutants...

10. The Future of Research Symposium Boston 2015

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    ...posing some of their own are the following panel members: Jonathan Dinman, Chair of the Cell Biology and...

11. New Tool for Lineage Tracing: The ClonTracer Library

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    ...library allows a researcher to label individual members of a starting population of cells with specifc,...

12. Plasmids for Endogenous Gene Tagging in Human Cells

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    ...technique that briefly destabilizes the cell’s outer membrane and allows the components to physically enter ...

13. CRISPR in the Clinic

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    ... in clinical trials includes Cas3 (the shredder member of the Cas family) as part of a cocktail therapy...

14. Viral Vectors 101: Virus Safety

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    ...biosafety cabinet. When decontaminating liquid waste, remember that the percentage indicates the proportion of...

15. Antibodies 101: Choosing the Right Isotype

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    ..., G. P., & Fisher, S. K. (1993). Chapter 15 Postembedding Immunocytochemical Techniques for Light and ...

16. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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    ...splice sites. Nat Biotechnol. Published online November 5, 2018. doi:10.1038/nbt.4283 Zibitt MS, Hartford...

17. Corralling and Cornering the Goldilocks Antibody

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    ...Zhuhao Wu and his colleagues! Dr. Wu and his lab members (like me!) are dedicated to creating a public and...

18. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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    ...your standard curve to plot to a linear equation (remember y = mx+b?). Figure 5: A standard curve ...

19. Overcoming the Challenges of Lentiviral Production

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    ...dish can be easily applied to multiple dishes, remember that handling multiple dishes will take longer...

20. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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    ...s research is the mechanisms by which proteins assemble into functional multi-subunit macromolecular complexes...