We narrowed to 626 results for: EMB

1. Transferable Skills Guide: Problem Solving

   Type

   Blog Post

   ...both in and out of the lab. The next step is to remember that you have these skills when you start applying...

2. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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   Blog Post

   ...multiplexed imaging of neurotransmitter, calcium, and membrane voltage,” says Tian. Figure 5: Citrine-tagged...

3. Viral Vectors 101: An Introduction to AAV

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   Blog Post

   ...expression for several years (Wonjo et al., 2013). Remember that AAVs do not integrate into the host genome...

4. The time and cost required to make a plasmid

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   Blog Post

   ... is a concern. The faster methods, like Gibson assembly, are typically more expensive, so you’ll have ...

5. Gendered Innovations: Why Does Sex of the Cell Matter?

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   Blog Post

   ...analysis for innovation and discovery. She is a member of the American Academy of Arts and Sciences and...

6. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

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   Blog Post

   ... their ability to cut the EMX1 locus in human embryonic kidney (HEK) cells, 11 of these initial mutants...

7. The Future of Research Symposium Boston 2015

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   Blog Post

   ...posing some of their own are the following panel members: Jonathan Dinman, Chair of the Cell Biology and...

8. New Tool for Lineage Tracing: The ClonTracer Library

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   Blog Post

   ...library allows a researcher to label individual members of a starting population of cells with specifc,...

9. Engaging with science and society at pgEd

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   Blog Post

   ...’ve since returned to pgEd as a full-time staff member for the past three years. pgEd is a small team ...

10. Overcoming the Challenges of Lentiviral Production

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    Blog Post

    ...dish can be easily applied to multiple dishes, remember that handling multiple dishes will take longer...

11. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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    Blog Post

    ...s research is the mechanisms by which proteins assemble into functional multi-subunit macromolecular complexes...

12. Adenoviral Vector Production and Troubleshooting

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    Blog Post

    ...packaging signal (Ψ), both of which are essential for assembly of the virion. Adapted from Lee et al., Adenovirus-mediated...

13. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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    Blog Post

    ...considerations for Split-BioID It’s important to remember that with Split-BioID, and BioID, any protein ...

14. Plasmids for Endogenous Gene Tagging in Human Cells

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    Blog Post

    ...technique that briefly destabilizes the cell’s outer membrane and allows the components to physically enter ...

15. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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    Blog Post

    ... with Cas9 interferes and prevents the correct assembly of the crRNA-Cas complex (Zhu et al., 2019). Inhibiting...

16. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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    Blog Post

    ...Addgene as a DNA Sequencing Analyst intern. As a member of the Quality Control team, I help make sure the...

17. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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    Blog Post

    ...splice sites. Nat Biotechnol. Published online November 5, 2018. doi:10.1038/nbt.4283 Zibitt MS, Hartford...

18. Corralling and Cornering the Goldilocks Antibody

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    Blog Post

    ...Zhuhao Wu and his colleagues! Dr. Wu and his lab members (like me!) are dedicated to creating a public and...

19. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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    Blog Post

    ...your standard curve to plot to a linear equation (remember y = mx+b?). Figure 5: A standard curve ...

20. Viral Vectors 101: Virus Safety

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    Blog Post

    ...biosafety cabinet. When decontaminating liquid waste, remember that the percentage indicates the proportion of...