We narrowed to 711 results for: ESC

1. Cpf1 Update: Comparison to Cas9 and NgAgo

   Type

   Blog Post

   ...for Cas9 (42 nt vs 100 nt.) However, the newly described non-CRISPR protein NgAgo is smaller than any of...

2. Tips for Titering Your Lentiviral Preps

   Type

   Blog Post

   ...is particularly useful when vectors carrying fluorescent markers are used as it eliminates the need for...

3. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

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   Blog Post

   ...familiar with the minute details of your work. Describe the problem by stating the facts and explain how...

4. How-to: 5 Steps to a Great Panel Discussion

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   Blog Post

   ...organizations, I asked audience members to stand and describe unique nonprofit models that differed from the...

5. 3D Printing Meets CRISPR Cas9

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   Blog Post

   ...reaction to this model on his blog. Or, for a description of how effective this model was in explaining...

6. AAV Vector Quality Control: Going the Extra Mile with NGS

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   Blog Post

   ...transduced cells whenever possible. This post will describe our workflow for viral genome sequencing (VGS)...

7. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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   Blog Post

   ...(the Gal4/UAS system), and the third post will describe how you can make your own mutant flies. Find Drosophila...

8. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

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   Blog Post

   ... assemblies per plate using outgrowth volumes described in Figure 3, with calculated yields from entire...

9. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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   Blog Post

   ...protein coding genes in the human genome with fluorescent proteins. For each gene, we needed to design ...

10. Of Myc and Men

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    Blog Post

    ...that perfect. One of the known limitations was described by Schüchner, et al., who published a paper showing...

11. Academic vs. Industry Postdocs

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    Blog Post

    ...academia, however, writing papers and grants is an inescapable and essential part of being able to continue ...

12. Addgene's Cancer Collection Pages Connect the Research Community

    Type

    Blog Post

    ...through several different applications such as fluorescent protein fusion studies and the generation of ...

13. Genome-wide Screening Using CRISPR

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    Blog Post

    ... are enriched using either drug selection or fluorescence-based cell sorting and screened for a particular...

14. Tips for Using BLAST to Verify Plasmids

    Type

    Blog Post

    ...matrix BLOSUM62, but you may want to check the description of the other matrices to see if another would...

15. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type

    Blog Post

    ... signal amplification in gene expression and fluorescence imaging." Cell 159.3 (2014): 635-646. PubMed...

16. Negative Can Be Positive: Open AAV Data with Addgene

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    Blog Post

    ...standards (clear and detailed methods and image descriptions, a minimum n = 2, and well labeled high- and...

17. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type

    Blog Post

    ..., P., & Berkmen, M. (2012). SHuffle, a novel Escherichia coli protein expression strain capable of correctly...

18. An Introduction to Adenovirus

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    Blog Post

    ...Early 3) genes deleted. These genes’ functions are rescued by helper plasmids used during production and/or...

19. Viral Vectors 101: The Retroviral Lifecycle

    Type

    Blog Post

    ...is a turn of phrase — “pseudotransduction” — to describe the direct protein transfer of RT, which would...

20. Course-Based Undergraduate Research Experiences (CUREs)

    Type

    Blog Post

    ...Histidine tag, malate dehydrogenase, and green fluorescent protein. Biochemistry and Molecular Biology Education...