Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 808 results for: Eng;

Showing: 521 - 540 of 808 results

  1. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...CRISPR-Cpf1 genome editing!  A recent paper from Feng Zhang's lab describes how to use Cpf1 for multiplex...spCas9: ~4 kbsaCas9: ~3 kb ~3.9kb crRNA/gRNA length gRNA: ~100 nt crNA: ~42 nt Requires tracrRNA?...

  3. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...fragment, Fvs are often produced recombinantly and engineered to be joined by a linker peptide, resulting in...Tomisaki M, Kiuchi T, Takagi J, Watanabe N (2022) Engineered fast-dissociating antibody fragments for multiplexed...

  4. To Each HIS Own

    Type
    Blog Post
    ...very pure sample. Because binding strength is proportional to the length of the tag, with longer tags you...

  5. All in a Twist: dsRNA

    Type
    Blog Post
    ...abundant in normal cells and are arise from a ssRNA engaging one strand of a dsDNA molecule; the 3-stranded..., but they also have a darker side. When ssRNA engages one strand of a dsDNA molecule, it does so at the...

  6. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...utilizes a gRNA which is photocaged and unable to engage a target sequence. Upon a brief light exposure ...Rosenberg, M., Hendel, A. Using Synthetically Engineered Guide RNAs to Enhance CRISPR Genome Editing Systems...

  7. Click Biology: How to Click and Play to Explore Biology

    Type
    Blog Post
    ...the utilization and development of genetically engineered components, like protein tags (Figure 2). Through...Tags Additional Resources on addgene.org New England Biolabs Cell-Imaging Plasmid Collection Promega...

  8. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...numbers of women participating in science and engineering. “Fix the Institutions” promotes gender equality...Gendered Innovations in Science, Health & Medicine, Engineering, and Environment—a project aimed at harnessing...

  10. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...and deliver a variety of cargoes, genetically engineered neurotropic viruses emerged as powerful tools...Watabe-Uchida et al., 2012). They could also quantify the strength of the inputs by counting the monosynaptic inputs...

  12. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ... Eukaryota can also harbor these circular DNA passengers. Most natural plasmids encode beneficial traits...Gateway cloning. In addition, scientists have engineered entirely new types of plasmids without clear ...

  14. How to Prepare for an Industry Interview

    Type
    Blog Post
    ... time out of their day to talk with you, so be engaged with their conversation. Send thank you emails ...carefully. And even if you don’t have technical challenges, your interviewers might.  You’ll probably still...

  15. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...safety features   When viruses were first being engineered as biological tools, thought was given on how...replication of virus and its infectivity in check. Engineering controls, safety systems that are built into ...

  17. Hot Plasmids February 2024

    Type
    Blog Post
    ...here!   Zhang, Z., et al. (2023). Efficient engineering of human and mouse primary cells using peptide-assisted...Researchers in the Gradinaru lab have developed an engineered adeno-associated virus (AAV) vector named AAV.CAP-Mac...

  18. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...Institute website at alleninstitute.org. A classic challenge in cell biology is making sure that what we observe...of the protein gleaned from the literature and engagement with researchers who study the protein or structure...

  19. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...tasks, both of which are factors in the technical challenges they can present. Many researchers find themselves....2 (2003): 141. PubMed PMID: 12705861. 3. Chen, Sifeng, et al. "Freeze-thaw increases adeno-associated...

  20. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...Hongkui Zeng Additional Resrouces on the Addgene Blog  Read about Other Means of Genome Engineering Using...
Showing: 521 - 540 of 808 results