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We narrowed to 989 results for: IGH@;

Showing: 521 - 540 of 989 results

  1. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    ...share? And how they are connected? Here are the highlights written as 8 tweetable facts. Note: The images...most Twitter users. Biologists rank #8. Figure 1: Highly Represented Scientific Disciplines on Twitter. ...disciplines: social (left), life (center), and physical (right). How do you use Twitter as a scientist? If you ...

  2. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

    Type
    Blog Post
    ...them share their reagents, we knew our role in fighting the pandemic could be even more impactful by reaching...scientists working in a for-profit setting.  In light of the need for basic research during the current...University of Iowa University of Minnesota See highlights from the COVID-19 collection Partnership with...

  3. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    ...Agrobacterium, along with a selectable marker. Left and right T-DNA borders specify the region of DNA that will...codon-optimizing the Agrobacterium ORI, they achieved much higher DNA yield and transformation efficiency in both...that gene expression from the M24 promoter is much higher than that from the commonly used 35S promoter. ...transfer. The M24 promoter provides constitutive, high-level gene expression and is functional in a variety...

  4. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type
    Blog Post
    ...been in space is pretty small). Therefore, I was delighted to be invited to the ceremony for surely what ...non-scientist from Bloomberg news. Given the topic it might have been better to have a scientist up there, but... viruses to give information to the computer to fight the known viruses. Bacteria have figured out how... virus sequence to enable them to recognize and fight it forever. He emphasized the potential of this ...

  5. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...research". The family entered the biomedical field’s spotlight after researchers discovered heavy-chain only ...reading frame Ability to access unique epitopes with high affinity, due to a greater ability to target concave...cellular functions, like in this early example we highlighted back in 2015, where sdAbs were used to create...

  6. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    ...toxic to both lines, it probably has either high toxicity or high off target effects, both of which make it... druggable target). Diseases with defined, straightforward causes (a specific mutation in a known gene...targets by CRISPR can accelerate the drug pipeline. High-throughput CRISPR screens can be used to inhibit...

  7. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ...Gates’ Foundation is involved with was one of the highlights of the conference. It was inspiring to see how...trusty spray bottles! We also raffled off one of our highly sought-after "nice genes…" tshirts, and the lucky...Fluorescence Labeling Plasmids from the Barbier lab). Bright and early on Saturday morning, John Doench’s opening...

  8. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    ...skills to get the work done efficiently and with high quality. Their work involves coordination and can...t mean going rogue, pointing out problems or highlighting criticisms. To be given the opportunity to take... by Peter Fuda and Richard Badham Leadership Insights for Engineers, Scientists and Computer Professionals...

  9. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...way? What ecosystem makes your heart sing with delight? From developmental biology to developing a career...example, how to use new technologies to reach the right audiences and how open access affects financial ...of scholarly publishing. And see for example the Eighth International Congress on Peer Review and Scientific...interested in the science communications world, you might consider an internship or a short program to build...

  10. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...neuroscience with ever more efficient tools for light mediated neuronal control and we are expanding our...target photostimulation of axons and dendrites on neighboring neurons. We have also packaged hChR2(H134R) plasmids...apparent dopamine affinity, with 113050 offering high apparent affinity, and 113049 offering medium apparent...glutamate levels. iGluSnFR.A184S, a variant with higher affinity to glutamate, has been packaged into AAVrg...

  11. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ...Bundling up for winter? The weather outside might be frightful, but browsing the 49 new preps available ...absolutely delightful. Grab a hot beverage and a blanket, then scroll on for some wintery delights!    Table...

  12. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    ...isotype converted, or chimeric, antibodies may be the right solution for you. Read on to learn more. What is...is an isotype? Antibodies are composed of light chains and heavy chains made up of variable regions and...097037-0.00017-8     Morelock, M. M., Rothlein, R., Bright, S. M., Robinson, M. K., Graham, E. T., Sabo, J...Polyclonal Antibodies Antibodies 101: Selecting the Right Antibody  ...

  13. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...planning to transform right away OR you can store the reaction at -20C overnight. 4. Transform TOPO Cloning...and requires no post-PCR procedures. Sounds easy right? The technique relies on the basic ability of complementary...efficiency).  5. Select and Analyze 10 White or Light Blue Colonies: You can confirm the presence of your...

  14. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ... Addgenie Julian Taylor-Parker explains why you might want to use a specialized E. coli strain for protein... to an entirely new career path, a career coach might be able to help you. Head Addgenie Joanne Kamens...genome, you need to check whether you’ve made the right one. In this post, Addgenie Melina Fan guides us...guides us through some common controls and why you might need them. Happy Reading!   Resources on the Addgene...

  15. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ...This guest blog post was contributed by Seán Mac Fhearraigh, PhD, of www.phdadvice.com. I sold out, well ...Like a considerable number of researchers, I had high hopes of securing tenure in a British or Irish University...interviewed for 1 hour so having a broad enough insight was going to help me push both my scientific and...death assays. You can find Seán on twitter @Macfhearraigh.    Read more posts about Scientific Career ...

  16. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ...is not just a question of being honest and straightforward, it’s an important part of being a “good scientific...work to figure them out. But, when they come to light, it’s clear that what the researchers thought was...still missing is a clear alignment of incentives. Right now, researchers are not strongly rewarded for being.... But it ought to be possible, and if we get it right, everyone in the scientific enterprise will benefit...

  17. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    ...involves measuring the absorbance/transmission of light through a liquid to determine the concentration ...liquid. Molecules absorb different wavelengths of light to different degrees and many molecules have a specific...method takes more time to set up in the lab, you might not have to do the calculation yourself as many ...disadvantages of each method and when one method might be more suited than another. Additional resources...

  18. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...to 3-loci MPE with similar high efficiencies, which is scalable for a higher number of MPE. Therapeutic...leader sequences of a given tRNA to achieve the highest efficiencies of multiplex editing at each site....Cas9-dependent off-target editing and do not cause higher Cas9-independent off-target editing in comparison...

  19. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...amplification and verification processes we use to ensure high-quality library distribution. You can also use these...amplification 101 Library amplification seems straightforward: E. coli bacteria are transformed with the ...a typical library, Addgene usually does four to eight electroporation reactions using a total of ~400ng.... Analyzing the reads is, again, seemingly straightforward – you need to count how many times each of ...

  20. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...protocol, get a result and hope it’s close to the right answer because if it’s not, well, your grade is ...the data look like... when it looks good, that’s a high that can last for several days.” Working with CRISPR...Doench thinks that the future of CRISPR will be in high throughput functional characterization of specific...plasmids, figure out what they do and what they might be able to do and to organize it and catalogue it...
Showing: 521 - 540 of 989 results