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We narrowed to 1,035 results for: LAS

Showing: 521 - 540 of 1035 results

  1. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...school system that has made strong efforts in the last 5 years to incorporate more science and technology...page in terms of the vocabulary--DNA, protein, plasmid, gene, etc. Though I talked about CRISPR’s molecular...should we use CRISPR? How shouldn’t we use it? This last section was arguably the most important, and it ...out our comprehensive CRISPR guide Find CRISPR plasmids and resources ...

  2. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ... donor DNA, and avoiding common mishaps in this class of genome edit. Knock-in mutations and gene editing...Targeted genome editing events fall into two general classes: knock-in and knock-out. The goal of a knock-out...experiment. Also learn about Addgene’s knock-in plasmids for reagents to help you knock your knock-in experiment...Cells Resources on Addgene.org Browse All CRISPR Plasmids Find Validated gRNAs for Your Next Experiment ...

  3. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ... serotypes in order to create an atlas of the mouse brain. This atlas enables researchers to quickly find...streptavidin-binding strep-tag and, more recently, OLLAS (Escherichia coli OmpF linker and mouse langerin...in particular are difficult to track with these classic probes. To overcome the limitations of existing...

  4. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...their MECP2-related plasmid tools with Addgene and share additional resources. Plasmid tools to facilitate...to partner with them to launch a Rett Syndrome Plasmid Collection and Resource Center for Rett Syndrome... Rett Syndrome in novel ways.  Addgene’s MECP2 Plasmid Collection and Resource Center will continue to...

  5. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ... usage bias in Plasmids 101: Codon usage bias Read more about plasmids in our Plasmids 101 blog series...Reference Read about genetic code expansion Browse our plasmid collection page  ...

  6. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ... at a particular hole (the active hole) induces laser stimulation of the chosen brain circuit, while a...most of its times in the opposite chamber) [2]. Laser activation is usually controlled automatically using...naturally so, after all the optimization, turn on the laser and watch what happens. Who knows, you may observe...optogenetics setup (viral expression, efficiency of laser light transmission to the target neurons, appropriate...

  7. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...used pairs to measure FRET. The table below lists plasmids that can be used to create your choice of fluorescent... fusion protein with your gene of interest: Plasmid Color Expression Description pPROEX Aqua Cyan...can easily directly excite the acceptor with the laser used to excite the donor. In that case the cross-talk...CRET and other RET techniques which have never outclassed the Master. Note: BRET= Bioluminescence Resonance...

  8. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...to whole-body in small animals, such as mice (Matlashov et al., 2020). Enhanced miRFPs are monomeric and...NIR FPs named miRFPs (monomeric infrared FPs) (Matlashov et al., 2020) derived from bacterial phytochrome...multicolor NIR imaging.  Small miRFPnano A separate class of NIR FPs, named miRFPnanos, were derived from ...ion indicator. Nat Methods 16, 171-174 (2019). Matlashov, M.E. et al. A set of monomeric near-infrared ...

  9. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...ever watched any behind the scenes footage in the last several years, you can usually see a director sitting...the subject after I’ve left the room. Microphone: Lastly, I needed to figure out a microphone situation....beating heart of Addgene. It’s why we exist, storing plasmids, and distributing them to labs that need them....a different shoot to create the How to Deposit Plasmids video. Shot without anyone else in the office....

  10. Evolution of Lab Techniques

    Type
    Blog Post
    ...techniques comes in handy whether you are perusing classic papers or are brainstorming new technological innovations...blotting, restriction mapping and sequencing gels are classic techniques that allowed for many seminal scientific... on Analyzing DNA Sequencing Results Check out Plasmid Cloning by PCR Resources on Addgene.org Check...Pages Find Molecular Biology Tools Analyze Your Plasmids by Restriction Digest ...

  12. Finding Your Perfect Job After University

    Type
    Blog Post
    ...finishing my MSc. The masters program was 6 months of classes and 6 months of hands-on lab work for a dissertation...would like to do. I had taken advanced virology classes in California and enjoyed them, so I applied for...found out the job was with Addgene. I had used plasmids from this company before and recognised the logo...experiments over the phone or explain how various plasmids work. Most people who call or email me are scientists...

  13. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...freezer, you’ll probably find lots of different plasmids containing the same protein of interest tagged...acid) that reacts with biarsenical dyes such as FlAsH (green, based on fluorescein) or ReAsH (red, based... Tetracysteine (Cys4) 15 Biarsenical dyes (FlAsH, ReAsH) Few Easy SNAP-tag 182 Benzylguanine...Addgene’s collection, so you may be able to get a plasmid ready-to-use without any cloning at all! As mentioned...

  14. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...Einstein and Bill Nye in their lab coats handling glassware with brightly colored hazardous liquids. Working...Sometimes teaches graduate and/or undergraduate classes Postdoctoral researcher (postdoc) Getting...responsibilities can vary "Lab assistants" usually wash glassware, prep media, etc. "Research assistants" are more...Feeling Like an Imposter: The Effect of Perceived Classroom Competition on the Daily Psychological Experiences...

  15. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ... Protocols Plasmid Cloning by PCR Plasmid Cloning by PCR You may...recipient plasmid (usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere on the plasmid. Bonus...recipient plasmid. Remember to insert your DNA in the correct orientation in the recipient plasmid by viewing... 1μg of recipient plasmid. It is also critical that as much of the recipient plasmid as possible be cut...ligations for each plasmid you are trying to create, with varying ratios of recipient plasmid to insert. It...recipient plasmid + insert plate should have significantly more colonies than the recipient plasmid alone ... on your recipient plasmid alone plate, you can try ligating the recipient plasmid alone in the presence...

  16. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ...Albany, NY, USA 42.6526°N 73.7562°W – Nicholas Thompson Over the last few years, there's been a disturbing...great, positive event that I hope happens again. Nicholas Thompson is a PhD Candidate studying Nuclear Science...Golden Hi, my name is Erin and I have spent the last 15 years training as an academic scientist. I’ve...

  17. Participate in the Pets of Addgene Scavenger Hunt

    Type
    Blog Post
    ...blog.  We’ve been mostly working remotely for the last year to give our lab and shipping teams enough space...made their way onto our Slack workspace over the last year. Now, we’ve collected photos of our pets with... You will find five photos this way. At the very last photo, you will find a link to a form where if you...

  18. We’re Going Viral This Week!

    Type
    Blog Post
    ...going viral,” we also have some other exciting news. Last week, we launched our new AAV Data Hub, an open ...production and data analysis. Our first paper came out last year on sucrose supplementation to increase AAV2...AAV2 yields and we’re eager to share more! Lastly, we’ll be at SfN2019. If you’re attending SfN2019, stop...

  19. Congrats to the 2019 Deck The Lab Winners

    Type
    Blog Post
    ...unseasonably warm weather we had last weekend). We’ve spent the last week perusing the Deck The Lab contest...2019 Hope you’ve enjoyed the festivities in the last month!  ...
Showing: 521 - 540 of 1035 results