Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 807 results for: MAK;

Showing: 521 - 540 of 807 results

  1. CRISPR in the Clinic

    Type
    Blog Post
    ...effectiveness, if the editing frequency isn’t high enough to make a physiological difference, then the treatment will...systems as well as improving delivery strategies to make fast, effective, and targeted therapy possible. ...

  2. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ... of MoClo. Image courtesy of Rob Hurt. What makes MoClo systems so powerful is their modularity — ...engineering complex cellular behaviors requires making complex manipulations to cells. For example: in...

  3. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...to CRISPR experiments, therefore, you often must make a choice about what is your top priority: location...trying to knock-in a patient mutation or need to make a precise amino acid change, there is very little...

  4. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...safety profiles as well as other benefits. What makes AAV vectors great gene delivery tools? There are...homology directed repair (HDR). This characteristic makes AAV vectors especially amenable to correcting disease-associated...

  5. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...low-cost, field-based diagnostics, our approach could make an impact. Our motivation was underscored by the... switches and amplification primers. This helped make sure that we had a number of successful sensors ...

  9. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ... humans (like many organisms) are opaque, which makes most biomedical applications of these tools difficult...purified. Although a ~1% change in signal might not make a very powerful tool, it was promising enough to...

  10. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...additional datasets used in the training of PRIDICT2.0 make this model more suited for prediction of very diverse...includes applying machine learning techniques to make sense of biology, with a special focus on gene editing...

  11. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...separates the two components of the indicator, which can make the kinetics of voltage sensing to signal output... (voltage sensing) hydrolyzes a precursor dye to make it more lipophilic, increasing its affinity for ...

  12. Of Myc and Men

    Type
    Blog Post
    ...used in way more applications.   Fig. 1 Making a Protein Tag (Abridged) (1) Locate a promising.... 1997; Schiweck, et al. 1997) with the goal of making the antibody more amenable to protein engineering...

  13. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ... inadequate plasmid DNA yields. Why would volume make a difference? In the simplest of terms, more volume...cells present in the given volume.  Let oxygen in! Make sure your culture is getting the right amount of...

  15. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ... learned along the way and hope they’ll help you make the most of the opportunities you’re provided throughout...erlenmeyer flask with gold glitter spray paint to make a trophy for the winner. We promoted the contest...

  16. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...made publicly available through Addgene.  What makes CRISPR so special? A major advantage of CRISPR/Cas9...  2) What genetic modification are you trying to make? Addgene carries CRISPR libraries for gene knockout...

  17. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...developments in microscopy coupled with techniques that make tissues transparent are enabling microscopes to ...point-by-point scanning along the entire specimen makes imaging too slow and unwieldy for large specimens...

  18. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...accurate, as these peptides are thought to function by making the ribosome skip the synthesis of a peptide bond...within the same multicistronic vector, effectively making a construct that expresses multiple unique genes...

  19. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...or validating your new clones, try these tips to make the most of your time and sequence! You might also...Standard Nucleotide BLAST page, the first decision to make is whether to compare a Sanger sequencing result...

  20. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...are of no use to you. When using more than one FP, make sure their emission light does not overlap in wavelength...eel was shown to mature independently of oxygen, making suitble for use in anaerobic conditions. Maturation...
Showing: 521 - 540 of 807 results