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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 817 results for: MAL

Showing: 521 - 540 of 817 results

  1. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...persistent immunological reconstitution with a normally functioning T cell repertoire (1). In this example...icosahedral, non-enveloped virus that carries a small, approximately 4.7 kb single stranded genome. AAV...

  2. Hot Plasmids February 2024

    Type
    Blog Post
    ... it with an “assist peptide” that facilitates endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA...editing frequency (nearly 100% in some cases) and minimal toxicity, including in clinically-relevant lines...

  3. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ...you. Once you input a sequence, SplitSet finds optimal fusion sites to split it into multiple fragments...antibiotic screening for selection, failed under normal cycling protocols, and required a 48-hour incubation...

  4. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...'t do it unless you can do it well" – even the smallest program. I don't mean have fancy food or a fantastic... put your panelists behind a table. It adds a formality and distance that prevents interaction with each...

  6. Transferable Skills: Negotiation

    Type
    Blog Post
    ...survey found that many researchers feel they lack formal training in a variety of transferable skills. At...easily work on this transferable skill without any formal training or coursework. First, you have to develop...

  7. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ... SNAPf-, CLIPf-, and CLAP-tagged proteins for mammalian cell expression. These plasmids will be useful...expresses all the components of the biosensor in optimal proportions from a vector backbone that allows ...

  8. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...dose (TCID50) (Zen et al., 2004).  To choose the optimal titering assay, you’ll need to understand how different...Levites, Y., … Golde, T. E. (2020). Utilizing minimally purified secreted rAAV for rapid and cost-effective...

  10. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...their versatile timer could be used in vitro, in mammalian cells, in C. elegans, and in Xenopus embryos. ... timers should therefore find use in studies of animal development where events like the patterning of...

  12. Of Myc and Men

    Type
    Blog Post
    ...five amino acids, meaning the tag could be quite small. However, substance P has an amide group added to...larger than the substance P peptide, but still quite small. However, unlike the substance P tag, they found...

  14. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...pCMMP-MCS-IRES-Puro IRES Retroviral pEF1a-IRES-Neo IRES Mammalian MSCV-IRES-Luciferase IRES Retroviral pWPI ...pWPI IRES Lentiviral AmCyan-P2A-mCherry P2A Mammalian pC5Kan-P2A P2A Insect pUltra P2A and T2A Lentiviral...

  15. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...amount and type of information you want to convey. Small tidbits and fast/fun facts are easy and engaging... be oversaturated. Transition from academic to informal writing can difficult.  Photography Medium...

  17. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...parties, and the company at large, by polling and informal communications we made a decision to try out a...the other hand if the journal club occurs with a small technical team (single lab or team), a deep technical...

  18. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...properly, it is unstable and may only work for a small number of qPCR runs before a new one is needed. ...and the droplet generation oil are moved through small channels to create a water-in-oil emulsion containing...

  20. CRISPR Antimicrobials

    Type
    Blog Post
    ...antimicrobial must reach essentially every cell or else small pools of antibiotic resistant cells could repopulate... systems used in both papers are analogous to mammalian viral vectors in that the vectors are replication-deficient...
Showing: 521 - 540 of 817 results