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We narrowed to 818 results for: Mal;

Showing: 521 - 540 of 818 results

  1. Hot Plasmids February 2024

    Type
    Blog Post
    ... it with an “assist peptide” that facilitates endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA...editing frequency (nearly 100% in some cases) and minimal toxicity, including in clinically-relevant lines...

  2. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ...you. Once you input a sequence, SplitSet finds optimal fusion sites to split it into multiple fragments...antibiotic screening for selection, failed under normal cycling protocols, and required a 48-hour incubation...

  3. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...endothelial cell-pericyte, neuron-astrocyte, and cancer-stromal cell pairs.   Figure 2: Schematic of the... fluorescent proteins (FPs) fused to the AID* (minimal 44 amino acids auxin inducible degron sequence)...

  4. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...persistent immunological reconstitution with a normally functioning T cell repertoire (1). In this example...icosahedral, non-enveloped virus that carries a small, approximately 4.7 kb single stranded genome. AAV...

  5. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...'t do it unless you can do it well" – even the smallest program. I don't mean have fancy food or a fantastic... put your panelists behind a table. It adds a formality and distance that prevents interaction with each...

  7. Transferable Skills: Negotiation

    Type
    Blog Post
    ...survey found that many researchers feel they lack formal training in a variety of transferable skills. At...easily work on this transferable skill without any formal training or coursework. First, you have to develop...

  8. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ... SNAPf-, CLIPf-, and CLAP-tagged proteins for mammalian cell expression. These plasmids will be useful...expresses all the components of the biosensor in optimal proportions from a vector backbone that allows ...

  10. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...their versatile timer could be used in vitro, in mammalian cells, in C. elegans, and in Xenopus embryos. ... timers should therefore find use in studies of animal development where events like the patterning of...

  11. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...dose (TCID50) (Zen et al., 2004).  To choose the optimal titering assay, you’ll need to understand how different...Levites, Y., … Golde, T. E. (2020). Utilizing minimally purified secreted rAAV for rapid and cost-effective...

  12. Of Myc and Men

    Type
    Blog Post
    ...five amino acids, meaning the tag could be quite small. However, substance P has an amide group added to...larger than the substance P peptide, but still quite small. However, unlike the substance P tag, they found...

  15. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...pCMMP-MCS-IRES-Puro IRES Retroviral pEF1a-IRES-Neo IRES Mammalian MSCV-IRES-Luciferase IRES Retroviral pWPI ...pWPI IRES Lentiviral AmCyan-P2A-mCherry P2A Mammalian pC5Kan-P2A P2A Insect pUltra P2A and T2A Lentiviral...

  16. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...can be used. The Fujii lab demonstrated this in mammalian cells by expressing dCas9 with an N-terminal 3xFLAG...expression of IGF2, a characteristic of many human malignancies. Additionally, the Tapscott Lab identified regulators...

  18. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...specifically to allow scientists to evaluate how small changes in vector administration can influence AAV...doi.org/10.1089/hgtb.2019.105 Rumachik, N. G., Malaker, S. A., Poweleit, N., Maynard, L. H., Adams, C....

  19. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...amount and type of information you want to convey. Small tidbits and fast/fun facts are easy and engaging... be oversaturated. Transition from academic to informal writing can difficult.  Photography Medium...

  20. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...higher Tm give more efficient recombination. We normally use a Tm between 47-55 °C for the homologous region...be very helpful to confirm primer design.  Use minimal template DNA Use 1 ng of PCR template DNA to minimize...
Showing: 521 - 540 of 818 results