We narrowed to 550 results for: NOL

1. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

   Type

   Blog Post

   ...CRISPR-Cas nucleases in human cells. Nature Biotechnology, 31(9), 822–826. https://doi.org/10.1038/nbt...

2. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

   Type

   Blog Post

   ...non-coding DNA region? To this end we developed a technology called CORRECT, which, by undergoing two rounds...

3. New Optogenetic Tools for Cytoskeleton and Membrane Control

   Type

   Blog Post

   ...activity. B) OptoTIAM (magenta) expressed in a monolayer of endothelial cells, showing cell protrusions...

4. Deep Mutational Scanning with One Pot Saturation Mutagenesis

   Type

   Blog Post

   ...protein function on a massive scale." Trends in Biotechnology 29.9 (2011): 435-42. PubMed PMID: 21561674. ...

5. Filming Science Videos in the Age of Social Distancing

   Type

   Blog Post

   ... at a 7-inch wireless monitor. Wireless video technology has gotten very impressive, but due to costs ...

6. Multiple Plasmids at a Low Price: Kits from Addgene

   Type

   Blog Post

   ...: Čelešnik, Helena, et al. "Biosafety of biotechnologically important microalgae: intrinsic suicide switch...

7. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

   Type

   Blog Post

   ... field, the lessons learned in pursuing one technological application can benefit many others and accelerate...

8. Treating Muscular Dystrophy with CRISPR Gene Editing

   Type

   Blog Post

   ...gene editing, but given the speed at which the technology is advancing, precision editing treatments may...

9. Hot Plasmids: Spring 2025

   Type

   Blog Post

   ...living cells and organisms with TurboID. Nature Biotechnology, 36(9), 880–887. https://doi.org/10.1038/nbt...

10. CRISPR 101: Off-Target Effects

    Type

    Blog Post

    ...effects in CRISPR/Cas9 gene editing. Front Bioeng Biotechnol. 2023 Mar 9;11:1143157. doi: 10.3389/fbioe.2023.1143157...

11. Viral Vectors 101: Optogenetic Tools

    Type

    Blog Post

    ...intracellular signaling pathways. Trends in Biotechnology, 33(2), 92–100. https://doi.org/10.1016/j.tibtech...

12. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type

    Blog Post

    ...Harvard University, Massachusetts Institute of Technology, Boston University, Johns Hopkins University,...

13. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type

    Blog Post

    ...Considerations For Precise Neuronal Targeting Using AAV Technologies Resources on Addgene.org Find ready to use...

14. With an Eye Towards the Future, We Look Back at the March for Science

    Type

    Blog Post

    ...cannot prosper in a world where our medicines, technologies, defense, and governing policies are developed...

15. Custom CRISPR Screens & the Green Listed Software

    Type

    Blog Post

    ... off-target effects of CRISPR-Cas9." Nature biotechnology 34.2 (2016): 184-191. PubMed PMID: 26780180....

16. Cancer and the Immune System: Deciphering the Relationship

    Type

    Blog Post

    ...from immunosurveillance to tumor escape." Nature immunology 3.11 (2002): 991-998. PubMed PMID: 12407406. ...

17. A Needle in a Base-Stack: Cas9 Structural Biology

    Type

    Blog Post

    ...RNA-programmed Cas9 nuclease specificity. Nature Biotechnology, 31(9), 839–843. https://doi.org/10.1038/nbt...

18. CRISPR Guide

    Type

    Guide

    ...egularly I nterspaced S hort P alindromic R epeat) technologies. Before CRISPR, genome engineering approaches...CRISPR one of the most scalable genome editing technologies, making CRISPR perfect for genome-wide screens...population, you will look for relevant hits using NGS technology. For more detail on using CRISPR libraries for...identified by in vivo screening in yeast. Nature Biotechnology , 36 (3), 265–271. PMID: 29431739 Chen, J. S...CRISPR-Cas nucleases in human cells. Nature Biotechnology , 31 (9), 822–826. PMID: 23792628 Fu, Y., Sander...specificity using truncated guide RNAs. Nature Biotechnology , 32 (3), 279–284. PMID: 24463574 Gao, S., Wang...specificity of RNA-guided Cas9 nucleases. Nature Biotechnology , 31 (9), 827–832. PMID: 23873081 Hu, J. H.,...

19. Molecular Biology Reference

    Type

    Guide

    ...synthetic amino acids. Plasmids and Recombinant DNA Technology Chemistry techniques enable the isolation and...specific DNA sequence. The field of recombinant DNA technology has overcome this obstacle by enabling the preparation...purification) and manipulation. With current cloning technology, it is easy to create and modify plasmids containing...editing is most commonly accomplished using CRISPR technology. CRISPR is composed of a DNA endonuclease and...plasmids used in the field of recombinant DNA technology have been optimized for studying and manipulating...thi-1 gyrA96 relA1 tonA EPI300 LGC Biosearch Technologies; MilliporeSigma F- mcrA Δ(mrr-hsdRMS-mcrBC) ...100 µg/mL Chloramphenicol 25 mg/mL (dissolve in Ethanol) 25 µg/mL Hygromycin B 200 mg/mL 200 µg/mL Kanamycin...

20. Optogenetics Guide

    Type

    Guide

    ...explanations of microbial opsin and optical switch terminology....governing biomolecular processes. The tools and technologies developed for optogenetics research utilize ... when combined with site specific recombinase technology like Cre-lox, which allows you to express an ... Schnitzer MJ. 2006. Next-generation optical technologies for illuminating genetically targeted brain ...