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We narrowed to 876 results for: NSI;

Showing: 521 - 540 of 876 results

  1. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ... Another caveat of the study is the method’s sensitivity. The authors estimate that a minimum of 150-200...necessary for a detectable signal, limiting the sensitivity of the technique. One potential solution is SunTag...

  2. Water Bath Protocol

    Type
    Protocol
    ...solution or a 70% ethanol solution to wipe down the inside of the water bath. Pro-Tip To properly maintain...maximum fill capacity is. Look for a “fill” line inside the water bath or read the user manual. If you’...may float in the water bath when they are placed inside. Water bath floats can be used to secure tubes ...before use, make sure that there is enough water inside....

  6. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...researchers around the world. We take this responsibility seriously. Every decision we make, every project... provides unmatched customer service that is consistently timely, informative, friendly, and professional...

  7. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...generally follow the same pattern described above - a sensing domain induces changes in a fluorescent signal ...compatible with your lab and experimental setup? Consider what kind of instrumentation you have available...

  8. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...a separate plasmid. This system amplifies the intensity of the fluorescent signal and enables single molecule...SunTag, they attempted single-molecule imaging deep inside the cell, in the nucleus and cytoplasm. Again, ...

  9. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...instead results in the breakage of the ester bonds responsible for the sugar backbone of the DNA helix or even...Storing DNA: temperature and longevity You should consider a couple of more things when you elute your DNA...

  10. Fostering communication in remote collaborations

    Type
    Blog Post
    ...a time. Just be sure there is a point person responsible for incorporating everyone’s suggestions into...timelines for experiments and manuscripts, who is responsible for which parts of the project, and, as mentioned...

  12. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    ...Chance to do/learn new things, diversity of responsibilities, which might include training or teaching ...Nature Biotechnology article “Managing knowledge-intensive workers”. I have added a few comments of my own...

  13. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...for your CRISPR genome engineering experiments, consider that wildtype Cas9 with optimized chimeric gRNA...screened? A4: Before starting your experiment, consider co-transfecting with GFP. This allows you to sort...

  14. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...most applications. Antibiotics vary in their sensitivitly to heat and/or light – do not add them to media...store plates/stocks wrapped in foil if a light-sensitive antibiotic like Tetracycline is used. Keep in ...

  15. Video Library

    Type
    Protocol
    ...Addgene's online resources. Career Videos Get career insight and advice from real Addgenies! Protocols (Link... once using a multichannel pipette and avoid inconsistent pipetting with our simple tips Bacterial Transformations...Australia, and advice for current graduate students considering their future careers. Eric J. Perkins, PhD In...Addgene's Business Development Manager, discusses his transition from the Scientist Team to Business Team, his...

  16. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...communication, I had the chance to carry out a comprehensive review of the literature informing the notion...science. Understanding just how the public utilizes insights gleaned from scientific research is, after all...

  17. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...find useful antibodies for your research. Please consider sharing your experiences with antibodies from .... The content of this article is solely the responsibility of the authors and does not necessarily represent...
Showing: 521 - 540 of 876 results