We narrowed to 568 results for: PAC;

1. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

   Type

   Blog Post

   ...decrease. Furthermore, the size of the parts also impacts ligation efficiency, as concentration does not ...

2. CRISPR 101: Any Base Transversion Editors

   Type

   Blog Post

   ...across from the abasic site before filling the empty space. Over several rounds of rational mutagenesis, the...

3. AAV Titers: Where Do They Come From And What Do They Mean?

   Type

   Blog Post

   ... of AAV-2 particles via a novel capsid ELISA: packaging of genomes can limit production of recombinant...

4. Career Insights: Technical Support Specialist

   Type

   Blog Post

   ...and can include the pressure to publish in high-impact journals, and the requirement for a high grant ...

5. Analyzing CRISPR Editing Results with ICE from Synthego

   Type

   Blog Post

   ...excluding the PAM sequence. PAM Sequence - The Protospacer Adjacent Motif (PAM) sequence for the nuclease...

6. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

   Type

   Blog Post

   ...high-resolution map of early embryogenesis in time and space, and was also used to precisely characterize developmental...

7. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

   Type

   Blog Post

   ...ready-to-use AAV from Addgene or check out protocols for packaging your own virus ...

8. Tips for Writing a Good Cover Letter

   Type

   Blog Post

   ...candidacy, beyond what is on your resume. It is a space for you to explain how your skills and experience...

9. Viral Vectors 101: Pseudotyping

   Type

   Blog Post

   ...plasmid, (2) the envelope plasmid, and (3) the packaging plasmid. These are transfected into target cells...

10. Viral Production at Addgene

    Type

    Blog Post

    ...cells using the 2nd generation psPAX2 and pMD2.G packaging system developed by the Trono lab. Cell culture...

11. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type

    Blog Post

    ...trans-splicing, and protein replacement to reverse the impacts of this disease.  As a non-degenerative, monogenic...

12. Mesothelioma - Causes, Symptoms, and Treatment

    Type

    Blog Post

    ...into mainstream use, they will have a much greater impact on the life expectancy and overall survival of ...

13. Tips for CRISPR Gene Editing in Mice

    Type

    Blog Post

    ...mice that may have no mutation, costing money and space. As a rule, screen all of your pups unless you have...

14. Rett Syndrome

    Type

    Collection

    ...trans-splicing, and protein replacement to reverse the impacts of this disease. Addgene is working with the RSRT...

15. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type

    Blog Post

    ...controls only address one type of error that can impact your western blot, and depending too much on normalization...

16. Tips for Getting a Faculty Position

    Type

    Blog Post

    ...research will advance the field, create a new field, impact disease treatment, etc., again in clear terms. ...

17. Hot Biosensors 2022: Year-End Roundup

    Type

    Blog Post

    ...size (~1500 bp) makes iGECInano suitable for AAV packaging.    Find iGECInano plasmids here!   Matlashov ...

18. CRISPR 101: Validating Your Genome Edit

    Type

    Blog Post

    ... editing, such as SpCas9-HF1, eSpCas9(1.1), or HypaCas9. Read more about Cas9 options in our blog post...

19. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type

    Blog Post

    ...the target site, while Cas12a requires a PAM (Protospacer Adjacent Motif) for target cleavage. This test...

20. Five Popular Model Organisms, Part 2

    Type

    Blog Post

    ...more model organisms that have left a historical impact on the scientific community. (And, check out this...