Addgene

We narrowed to 556 results for: PAC;

Showing: 521 - 540 of 556 results

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Analyzing CRISPR Editing Results with ICE from Synthego

Type

Blog Post

...excluding the PAM sequence. PAM Sequence - The Protospacer Adjacent Motif (PAM) sequence for the nuclease...
AAV Titers: Where Do They Come From And What Do They Mean?

Type

Blog Post

... of AAV-2 particles via a novel capsid ELISA: packaging of genomes can limit production of recombinant...
Career Insights: Technical Support Specialist

Type

Blog Post

...and can include the pressure to publish in high-impact journals, and the requirement for a high grant ...
Tips for CRISPR Gene Editing in Mice

Type

Blog Post

...mice that may have no mutation, costing money and space. As a rule, screen all of your pups unless you have...
Antibodies 101: Normalization and Loading Controls for Western Blots

Type

Blog Post

...controls only address one type of error that can impact your western blot, and depending too much on normalization...
Tips for Getting a Faculty Position

Type

Blog Post

...research will advance the field, create a new field, impact disease treatment, etc., again in clear terms. ...
SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

Type

Blog Post

...the target site, while Cas12a requires a PAM (Protospacer Adjacent Motif) for target cleavage. This test...
Deep Mutational Scanning with One Pot Saturation Mutagenesis

Type

Blog Post

...into newly synthesized DNA. This labeled ssDNA is packaged into phage and then extracted. Besides taking ...
Five Popular Model Organisms, Part 2

Type

Blog Post

...more model organisms that have left a historical impact on the scientific community. (And, check out this...
Hot Biosensors 2022: Year-End Roundup

Type

Blog Post

...size (~1500 bp) makes iGECInano suitable for AAV packaging. Find iGECInano plasmids here! Matlashov ...
CRISPR 101: Validating Your Genome Edit

Type

Blog Post

... editing, such as SpCas9-HF1, eSpCas9(1.1), or HypaCas9. Read more about Cas9 options in our blog post...
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

Type

Blog Post

...development of a new technique. In a structure as tightly packed and interwoven as the brain, robust single-cell...
Is this the right place for me? 8 tactics for choosing a lab

Type

Blog Post

... this. The training you get now will have a big impact on everything you do in the future. Questions to...
Plasmids 101: Restriction Cloning

Type

Blog Post

... important to have nice crisp bands and to have space to cut out the bands. Because of this we recommend...
Finding Your Perfect Job After University

Type

Blog Post

...I really loved the fact that my work had a real impact people’s lives and would improve their treatment...
Brain Initiative Collection

Type

Collection

...65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine AAV packaging vector for hSyn driven KOR DREADD expression. ...
Deep Dive: Statistical Tests (Comparisons)

Type

Blog Post

...independent variables. For example, you could test the impact of temperature, humidity, sunlight exposure time...
Antibodies 101: Validation

Type

Blog Post

...specificity. However, there are countless variables that impact an antibody’s affinity and specificity for a given...
RNA Interference in Plant Biology: New Tools for an Old Favorite

Type

Blog Post

...the most prevalent method due to ease of use and capacity to generate a long-term RNAi phenotype. Long hairpin...
Custom CRISPR Screens & the Green Listed Software

Type

Blog Post

...GC%, reverse compliment sequences, and more. Output_Compact: A text file that describes which genes were...

Showing: 521 - 540 of 556 results

Results per page:

Analyzing CRISPR Editing Results with ICE from Synthego

AAV Titers: Where Do They Come From And What Do They Mean?

Career Insights: Technical Support Specialist

Tips for CRISPR Gene Editing in Mice

Antibodies 101: Normalization and Loading Controls for Western Blots

Tips for Getting a Faculty Position

SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

Deep Mutational Scanning with One Pot Saturation Mutagenesis

Five Popular Model Organisms, Part 2

Hot Biosensors 2022: Year-End Roundup

CRISPR 101: Validating Your Genome Edit

Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

Is this the right place for me? 8 tactics for choosing a lab

Plasmids 101: Restriction Cloning

Finding Your Perfect Job After University

Brain Initiative Collection

Deep Dive: Statistical Tests (Comparisons)

Antibodies 101: Validation

RNA Interference in Plant Biology: New Tools for an Old Favorite

Custom CRISPR Screens & the Green Listed Software

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