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We narrowed to 1,015 results for: PLE

Showing: 521 - 540 of 1015 results

  1. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...Fluorescent proteins (FPs) offer scientists a simple yet powerful way to tag cellular proteins and investigate...another (e.g. green to red, or cyan to green). For example, mEosFP (named after the goddess of dawn in Greek...state (“quenching”). The photoswitch can occur multiple times by activating with two distinct wavelengths...trafficking and dynamics (fission, fusion, etc). For example, by engineering PA-GFP tagged to a mitochondrial...relies heavily on the same fluorophore being imaged multiple times in order to reconstruct an image. The best...variants are more monomeric than mEos2 (mEos4b is completely monomeric) and display bright and photostable...

  2. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...Binghamton University Cancer is one of the greatest examples of survival of the fittest. Cancer cells find ... use the blood stream to commute to and invade multiple organs, and most importantly escape the immune... also known to precede cancer development, for example, inflammatory bowel disease is associated with ...the expression of the major histocompatibility complex (MHC) class I proteins on their surfaces to avoid...therapies must be explored. When attacking cancer, multiple immunotherapy targets can be utilized at the same...and suppression of tumor-aiding cytokines, for example, will more broadly activate the immune response...

  3. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ... learning behavioral optogenetics in order to implement the technique in my lab at The Rockefeller University...physiology in order to directly show that, for example, the firing of a specific type of neuron in a specific...too but that’s what collaborators are for). For example, if you study neural pathways involved in addiction... proper controls are required and include, for example, confirmation of opsin expression in the intended...you may need to allow the virus to express. For example, if you inject into the ventral tegmental area ...22055387;  PMCID: PMC3225502. 4. Mattis J, et al. Principles for applying optogenetic tools derived from direct...

  4. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...and therefore higher titer will be required. For example, retrograde AAV gets transported up the cell’s ...titers for successful transduction. In another example, neuronal gene expression by an anterograde AAV...visualize expression of fluorescent proteins, for example, as many viral particles must infect a cell to ... can optimize AAV production. We’ve listed some simple steps you can take below but you should read our...possible, use low-bind or siliconized tubes to store samples as it is more difficult for viral particles to ...

  5. The MTA at Addgene

    Type
    Blog Post
    ...more consistent, and more pleasant for everyone, which in turn encourages people to share more, since they...positive feedback on “our” MTAs, which sometimes makes people think our MTAs are different than other institutes...has placed a request for your plasmid, they must complete a Recipient Scientist Acknowledgment (RSA), which...use for the requested material. Once the RSA is completed, the recipient organization is sent the MTA to...

  6. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    ...efficient product of HMOs that could be used to supplement newborn diets and promote the formation of a ...breaks down the HMO of interest into its component simple sugars (including lactose), and a lac operon controlled...to determine the amount of HMO originally in the sample. The second sensor is a fusion protein consisting...researchers to engineer healthier microbiomes in people all over the world. Toward this end, she recently...training and wants to make her work understandable to people both inside and outside the scientific community...

  7. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    ...equivalent of crossing off days on the calendar after completing your writing goal, which can serve as positive...graph of which days of the week you most often completed your habit is useful for figuring out when to ...makes suggestions on how to make your writing less complex. While I don’t think you should accept all of the...jargon in your writing. While it’s not possible to completely avoid jargon, this tool can help you avoid overusing...tools for grad school Hello PhD Podcast episode 15 "Simple tricks for time management: the pomodoro technique...

  8. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ...make socially distanced work more creative. An example could be making lab meetings more interactive and...and brainstorm! Image from Joseph Mucira.   Implement new tools for creative interaction During Zoom...promote creative interaction. A great tool that a few people use is the built-in whiteboard, in which participants...GA, O’Reilly CM, Lofton ME, Catalán N (2018) Ten simple rules for collaboratively writing a multi-authored...journal.pcbi.1006508 Vicens Q, Bourne PE (2007) Ten Simple Rules for a Successful Collaboration. PLoS Comput...

  9. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...lentivirus production frustrating - a researcher can complete an entire lentiviral production protocol only ...material pages associated with each item. There are multiple CRISPR tools available as lentivirus and we’ll...ll be adding more as the need arises. For example, we’ve teamed up with the Broad Institute to provide...the publications associated with these libraries. Please note, the pooled libraries we distribute in lentivirus...or are thinking about using our viral services. Please send any questions or comments to help@addgene....

  10. 5 Great Apps for Lab Life

    Type
    Blog Post
    ... counter, lab timer, dilution calculator, and a couple of other nice features. This app also has a great...universally useful. 4. Chemical Safety Data Sheets by ThatsMyStapler Safety always comes first with research! It...pocket.   5. +AMINO- by iGreenEarth AMINO is a very simple app that packs important biological information...can safely say that I still have the single and triple abbreviations of the amino acids memorized, but...researchers in the lab. If you have a favorite lab app, please share it in the comments section below! Many thanks...

  11. Components of CRISPR/Cas9

    Type
    Blog Post
    ...to the Cas9/tracrRNA complex. D) The mature crRNA is used to guide the Cas9 complex to the target DNA which...functions of the various CRISPR/Cas9 components. Please note that while this post is intended to provide...requirements of these different systems can vary (for example, xCas9 is a variant with increased PAM flexibiliy...remainder of this post, we’ll refer to this RNA complex as the “gRNA”. The gRNA contains both the 20 nucleotide...

  12. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...Sharp. After a few rotations, Doench decided to complete his PhD work in Sharp’s lab.  Doench says, “what...what I loved about [RNAi], ... it was just this complete black box. The RNAi process had just been discovered...he says, “In the case of CRISPR, the hype was completely justified… it has been just an incredibly useful... [at the Broad] too… to make science easier for people everywhere…. the nonprofit, sharing ethic that ...comb through the literature, to go and talk to the people who actually have these plasmids, figure out what...

  13. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...chronologically. With a resume however, most often people choose a reversed chronological style (the latest...and tailor the resume for the job posting. For example, if the job asks for experience in “statistical...necessary in order for you to do your research. For example, did you help put together a grant application?...be meaningful for your potential employer. For example, my experience in science outreach and writing ... into a communications role instead after she completed her MSc in Experimental Medicine. She is the Community...

  14. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...cell imaging experiments is the ability to follow multiple fluorescently tagged species simultaneously. To...do so with fluorescent protein labels requires multiple fluorescent proteins whose excitation and emission... proteins should be separated by 50-60 nm. For example, CFP (ex 430 nm / em 474 nm) and YFP (ex 514 nm...protein of interest has a similar spectrum. For example, here's a screenshot from the Chroma Spectra Viewer...biliverdin as a cofactor and so you may need to supplement your cells with biliverdin for maximal brightness...

  15. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ... with your CV to people you’d like to work with next.” Connie Cepko looks for people who are dedicated... and interested in science. “Postdoc hires are simpler because of their documented history in science....satisfaction (especially for scientists and technical people) is linked to engagement and access to growth opportunities...Finding your dream lab While none of us will feel completely happy at work all of the time, the goal is to...

  16. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...interact with each other. Chromatin regulation is complex and influences--directly or indirectly--many other... we definitely don’t have that you really want, please let us know! And if you study chromatin and want...t see the gene you’re looking for on this list, please consider searching directly on our website. ...phosphoinositol-dependent binding of the SWI/SNF-like BAF complex to chromatin after T lymphocyte receptor signaling...not the first Addgene blog post about chromatin--multiple Addgene colleagues and guest bloggers have written...

  17. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...somewhat more reliable quantitation. Differences in sample treatment across methods can affect reported titer...that users keep titering method consistent across samples within a project. Targeting Your AAV Q4: Are there...for very high MOI on top of very high coverage. Please see this paper for more information about PHP.eB... will very likely cross-react with each other. Multiple labs have tried to transfer PHP.eB's enhanced ...which may be linked to epigenetic changes, such as multiple sclerosis?  A: Basic science and preclinical researchers...

  18. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ... For example, setting up your pipette tips to match your plate layout can help you track samples as you...change variables to fix a failed experiment.  For example, I used to constantly underestimate time needed... and make a checkmark next to each step as you complete it. Yes, this can be incredibly tedious, but it...remember what's been added and what hasn't. Developing simple habits like these can consistently reduce errors...

  19. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    .... This luminescent technology is based on the principle of bioluminescence resonance energy transfer (...photobleaching/phototoxicity, they successfully imaged multiple subcellular compartments over several minutes ...improve long-term imaging, which is limited by depletion of luminescent substrate, they synthesized diacetyl... handy. This approach is especially suited to complement optogenetic tools, where light used for excitation...external light source. Therefore, especially thick samples may not be appropriate for use with Nano-lantern...

  20. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...Cis/trans activity, PAM requirement, gRNA, and multiplex ability Experimental guidelines and applications... the cis and trans activity of each entry. For example, SpyCas9a (one of the most frequently used Cas ...you need more nitty-gritty details — how can I multiplex this enzyme? What other RNA components are required.... For each enzyme, it will also show you how multiplexed arrays would be designed, if that’s your end ...details to refine your search (trans activity, multiplex ability, gRNA design, etc.). With this feature...
Showing: 521 - 540 of 1015 results