We narrowed to 663 results for: PPL;

1. Screening for Successful Genome Editing with Digital PCR

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   Blog Post

   ...a gene of interest is one of the most common applications of precision genome editing, the remainder of...

2. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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   Blog Post

   ...cleaved by Cas9 and repaired via HDR using the supplied donor plasmid will express GFP. If there’s GFP...

3. Sharing is Caring: But How to Distribute Open Hardware?

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   Blog Post

   ...Challenges such as the provision of an accessible supply chain for parts that are listed in the bill of ...

4. How Addgene Creates Content

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   Blog Post

   ...antibody and content team, we identified what applications should be included and what basic information...

5. Plasmids 101: Screens vs. Selections

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   Blog Post

   ...require there to be a culture condition that can apply enough pressure to select for variants of interest...

6. Antibodies 101: Isotypes

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   Blog Post

   ... Isotype controls are used in antibody-based applications including flow cytometry, immunohistochemistry...

7. Viral Vectors 101: Inverted Terminal Repeats

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   Blog Post

   ... of research is important for downstream AAV applications.  Tips & FAQs Due to their high GC content and...

8. Plasmids 101: Inducible Promoters

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   Blog Post

   ... stimulate AraC binding to I1 and I2 sites. Supplementing cell growth media with glucose decreases cAMP...

9. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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   Blog Post

   ... SpCas9. In order to expand the gene-editing applications and targeting scope of ABEs David Liu’s lab ...

10. Developing a cold tolerant E. coli using specialized chaperones

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    Blog Post

    ...Additionally, we’re also working towards widening the applicability of our strategy using the pMBP-LbCas12a and ...

11. 15 Years of Addgene: The Top 15 Plasmids

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    Blog Post

    ...cell types so it is useful in a wide range of applications. This plasmid was the top requested plasmid ...

12. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

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    Blog Post

    ...directed evolution to create APEX2. They have now applied the same directed evolution technique to the biotin...

13. Top Requested Lentivirus and AAV of 2016

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    Blog Post

    ... preps. Cas9 is the headliner of most CRISPR applications and can be expressed from your cell line of ...

14. How to Keep a Lab Notebook for Bioinformatic Analyses

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    Blog Post

    ...in my own research. Again, some of this may not apply or may not work for you. The best way to find a ...

15. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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    Blog Post

    ...assembly methods are preferred. No such restriction applies to deletions; using REPLACR-mutagenesis, Trehan...

16. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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    Blog Post

    ..., or words of advice for people who may want to apply this tagging system to their own work? Mendenhall...

17. Plasmids 101: Blue-white Screening

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    Blog Post

    ...certain media components, and are rescued by genes supplied on the transformed plasmid. Such plasmids may ...

18. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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    Blog Post

    ...analyzing the data. It should be straightforward to apply the new library for use in various cell types and...

19. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

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    Blog Post

    ...competitions. After much learning, the focus of the application was changed, but Sample6 still leverages the ...

20. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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    Blog Post

    ...response to blue light (445nm). The discovery and applications of protein partners that bind to only the light...