...the issue is not unique to IHC.  References and Resources References Goodpaster T, Randolph-Habecker J...endogenous IgGs in the tissue. Created with biorender.com. Strategies for avoiding background secondary...them to your sample so the secondary antibodies aren’t free to bind endogenous IgGs. (C) A third solution...primary and secondary antibodies. Created with biorender.com. Proper tissue processing and sample preparation...

...this emerging technology! Resources and References References Orguc, S., Sands, J., Sahasrabudhe, A., ...electrophysiological potential afterwards. This measurement trended with expression of the opsin in the target neurons...obvious next applications are to expand the tool to different brain-organ axes. Alternatively, the system could...Free Behavior. 2020 42nd Annual International Conference of the IEEE Engineering in Medicine & Biology...

...Argonaute proteins play an important role in RNA interference, binding RNA guides to cleave foreign RNA. Argonaute...guides/gene for 10 genes, they did not observe differences in cleavage efficiency. If this finding is replicated...) or staggered (Cpf1) cleavage. NgAgo takes a different approach, randomly removing 1-20 nucleotides from...Read About NgAgo User's First Impressions Here References 1. Gao, Feng, Xiao Z. Shen, Feng Jiang, Yongqiang... 2. Swarts, Daan C., et al.  “DNA-guided DNA interference by a prokaryotic Argonaute.” Nature. 507(7491...

...transition from a clump of cells to a fully differentiated body. In contrast, these dynamic processes ...distinguishing cell populations that have been tagged with different fluorescent proteins or dyes. 3. Two-photon microscopy...cells. For example, by harvesting lymph nodes at different times after antigen challenge, scientists were... see movement and interaction between several different immune cells. These methods allowed immunologists... like this is a mosaic of cells labelled with different fluorescent proteins. Using one iteration of this...understanding of the immune system and its dynamics. References 1. Peters, Nathan C., et al. "In vivo imaging...

...isotype is expressed at different times of the immune response and initiates different immune cascades. In...In the research setting, antibodies of different isotypes can be used together in the same experiment ...However, each animal will make antibodies against different protein epitopes and so variability between lots...types of antibodies are out there, and how many different research applications they can be used for. Addgene... - and we’re always growing our collection! References: Arbabi-Ghahroudi M (2017) Camelid Single-Domain...

...globe, it can be a bit of a mystery for those who aren’t familiar with it. If you’re curious about the hows...is shared between two parties. There are many different flavors of MTAs, but the Uniform Biological Material...which sometimes makes people think our MTAs are different than other institutes. But they’re not! The vast...perfect, but it does provide consistency and transparency in an otherwise messy process.  And you do have..., instead of coordinating signatures from two different institutes. Our online portal automates a lot ...

...that replicate current knowledge, but the sensor can also kinetically outperform current methods. Unger...intended to help early career scientists attend a conference of their choice to share their fluorescent protein...are then extracted and sequenced. Chiasson is currently using VAMP-seq to study vitamin K epoxide reductase...and rapidly test the variants in vitro against different drugs and dosages.  Michal Bernach: Developing...

...have risen into yeast expertise! References and Resources References Liu, Wei, et al., From Saccharomyces...lists some of the biological commonalities and differences between K. phaffii and other yeast strains.   ...Applications for K. phaffii There’s a number of different uses for the phaffii-lous strain. Protein expression...The yeast giants - S. cerevisiae and S. pombe - aren’t going anywhere anytime soon, and for good reason...

...for creative interaction During Zoom meetings, different tools can be used to promote creative interaction...as a picture. I believe that showing something different from time to time breaks up the monologue a little...papers will be written by several authors from different institutes, disciplines, and cultural backgrounds...and only general chatter will be allowed with a different host each time that moderates conversations. This...communities. You can follow him on twitter @salmon_phd References Frassl MA, Hamilton DP, Denfeld BA, de Eyto E...

... the library are differently sized, smaller plasmids may be amplified preferentially, skewing the composition...successful amplification? To provide a point of reference, Addgene commonly prepares NGS samples using both...overrepresented plasmids (plasmids that were amplified preferentially). You can also rank the plasmids by abundance...create a “cumulative wealth distribution” curve, or Lorenz curve. By calculating the area under the curve ...

...based on the predicted sizes and organization of different features within the plasmid. Restriction analysis...can search NCBI for YGOI to find the particular reference sequence if necessary. The example plasmid on ...fragment having increased intensity to serve as a reference band. The uncut DNA (lane 2) shows 3 possible ...in the gel well and the dyes provide a visual reference point so you can easily assess how far the gel...Eductional Resources: Addgene's Molecular Biology Reference Find More Protocols and Videos      ...

...far the T4 exonuclease proceeds: at the first occurrence of the chosen nucleotide, T4 will switch to polymerizing...efficiently repair the plasmid. Created with BioRender.com.   SLIC also opened up a new world of multicomponent...bacteria, which repair the plasmid. Created with BioRender.com. How does SLIC compare to other cloning ...method’s flexibility allows it to be used with different types of PCR-generated inserts. By harnessing ...updated by Emily P. Bentley in September 2024.  References and Resources Further reading Harnessing homologous...

...organizers. My overall impression from this conference was that Cancer Researchers and Immunologists...Here I aim to compile a few highlights from the conference which reflect how cancer/immunology researchers...multi-pronged attack. Inflammation and tumorigenesis The conference opened with a very interesting presentation by...our Fluorescent Protein Guide. Throughout the conference aspirin and the use of COX inhibitors was discussed...about our free deposit and distribution service! References: 1. Pietrocola, Federico, et al. "Caloric restriction...

...Sanders of ComSciCon, the Communicating Science Conference series for graduate students. I believe that...giving a public talk, ask a friend who works in a different field to listen to you practice.  Ask them what...organizations. At ComSciCon, our primary mission is to strengthen and support the network of graduate students ...addition to this annual event, graduate students in different regional and disciplinary communities gather at...Learn about careers in science communication The strength of story telling Science communication: 9 strategies...

...chromosomal domains, or how stretches of DNA on different chromosomes interact with each other. Chromatin...Architecture and Chromosome Organization. These aren’t the only investigators in the chromatin field who... Note that histone genes frequently have many different variant names, not all of which can be listed ...Hutchinson JN, Sara SA, Ensminger AW, Fox AH, Chess A, Lawrence JB Mol Cell. 2009 Mar 27;33(6):717-26. Reporter-nanobody...me at the Keystone Chromatin meeting, especially Karen Reddy (@theReddyLab), Thoru Pederson, Joanna Wysocka...

...tools to further their research. However, tools aren’t very useful if you don’t know how to use them. ... ideas come from social media conversations, conferences, and questions our scientific support staff receives...line with Addgene style.        Fig. 2: Reference image (left) provided to our graphic designer ...for our blog posts, we usually make figures in BioRender or use appropriately licensed and cited figures... We are constantly reviewing the resources we currently offer to identify gaps and add to them as techniques...

... a screening strategy as well. In a screen, you aren’t killing off a portion of the cells like in a selection...You’ll want to pick enzymes that will give you different sized bands depending on whether the plasmid contains...using vector-specific primers, look for size differences. You can also use primers where one primer anneals...popular sequencing primers in our Molecular Biology Reference. Sometimes common  primers can't provide all of... Sanger sequencing Read the Molecular Biology Reference ...

...developers work in a streamlined manner. Now, in my current role at Oxford Nanopore Technologies, I work with... part of my job is getting to talk to so many different people! I communicate with customers, software...Often, product managers will have three or more concurrent projects in varying stages, which can get messy...meetings. Meet with my boss and coworkers, discuss current and future features being built for the analysis...blogger Rachel Rubinstein! Rachel Rubinstein is currently a Field-based Product Manager at Oxford Nanopore...

...commonly used in research laboratories to detect the different subclasses, but there are also secondary antibodies...secondary antibodies to detect multiple targets with different mAbs in the same experiment. The Trimmer Lab found...within the plasmid backbone to generate R-mAbs of different subclasses, including subclasses that were not... useful research materials and information. References and Resources Additional Resources on the Addgene...Addgene's recombinant antibody plasmid collection References Andrews NP, Boeckman JX, Manning CF, Nguyen JT...

...the wrong everything else.      References and Resources References Boston University Flow Core. Fluorochromes...standard curve, another quick experiment, or using a different vial and saving the poor, abandoned antibody for...assays involve multiple antibodies, each with a different signaling molecule, that all need to be detected...   Fig 2: Try to inversely pair fluorophore strength with protein abundance.    Second, you’ll ...