We narrowed to 583 results for: SUB;

1. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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   Blog Post

   ...PMC5681539. Additional resources on the Addgene Blog Subscribe to CRISPR blog posts Learn about other CRISPR ...

2. AAVs CREATed for Gene Delivery to the CNS and PNS

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   Blog Post

   ...AAV as a tool for gene expression in mammals. Subscribe to Viral Vector Blog Posts Additional Resources...

3. Negative Can Be Positive: Open AAV Data with Addgene

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   Blog Post

   ... from Addgene and the CLOVER Center, each one submitted by researchers like you. Reports are reviewed ...

4. Summer SciComm Series: Modes of Communication

   Type

   Blog Post

   ...kind of content are you sharing?  Sometimes your subject matter will drive the kinds of communications you...

5. Lentiviral Vector Uses and Overview

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   Blog Post

   ...VSV-G due to wide infectivity, is often used as a substitute for HIV-1 Env, which can only infect CD4+ cells...

6. CRISPR Antimicrobials

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   Blog Post

   ...and potency with phages Neither group observed substantial resistance to the CRISPR antimicrobials, and ...

7. Tips for Screening with Yeast Two Hybrid Systems

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   Blog Post

   ...entire proteome of a particular cell type, or a subset based on a cellular compartment or growth stage...

8. Cpf1: A New Tool for CRISPR Genome Editing

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   Blog Post

   ...nuclease to mediate cleavage rather than the multi-subunit complex employed by class 1 systems. A putative...

9. Tips from the Repository Trenches: Using Barcodes to Track Samples

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   Blog Post

   ...transformation and storage begins. Some samples are submitted as bacterial colonies in petri dishes, but close...

10. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    Blog Post

    ...spontaneously and without additional co-factors, substrates, or enzymatic activity – it only requires the...

11. Twenty Years of Sharing: Addgene's Viral Vector Service

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    Blog Post

    ...protein tools that are activated, respectively, by substrates, light, or engineered ligands (Figure 2). Biosensors...

12. Hot Plasmids Spring 2024

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    Blog Post

    ...-to-noise ratio and better ability to record subthreshold voltage fluctuations, while having high fidelity...

13. Plasmids 101: Broad Host Range Plasmids

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    Blog Post

    ...Enterobacter sp., Erwinia amylovora, E. carotovora subsp. Carotovora, E. herbicola, E. rubrifaciens, E. stewartii...

14. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

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    Blog Post

    ...doxycycline-free media and expand the cells. Use a subset of the cells to verify the mARG positivity, which...

15. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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    Blog Post

    ...patients from whom the blood is obtained and the subsequent reprogramming success [1]. Fibroblasts need to...

16. Transferable Skills Guide: Creativity

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    Blog Post

    .... It is important to note that the person who submitted the idea did not have to be in charge of implementing...

17. Antibodies 101: Immunoprecipitation

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    Blog Post

    ...antibody that will be used for detection in a subsequent Western blot. When choosing beads, there are ...

18. Viral Vectors 101: Pseudotyping

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    Blog Post

    ...rabies dG enters this neuron, it cannot infect any subsequent neurons because it does not express rabies glycoprotein...

19. CRISPR-based Genome Editing Systems For Editing in E. coli

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    Blog Post

    ...resulting in cutting of the plasmid. At this point, subsequent introductions of pTargetF containing another ...

20. Hot Plasmids - August 2020

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    Blog Post

    ...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...